FastQCFastQC Report
Thu 26 May 2016
SRR1544731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544731_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1125674
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82870.7361811679047398No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64190.5702361429685682No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT29550.2625093943717275Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27390.243320890417652No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT26900.2389679427614034No Hit
TTTTGAGGGATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCAT12860.11424266705991255No Hit
TTCTGCGGGAGGAAATTATTGAAAGTTTGGGAGGGACTATTCACAGTATAGA11710.10402656541769642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA351.01954356E-746.0000048
CGTATTA351.01954356E-746.00000442
CGACCCC351.01954356E-746.00000420
CACGAGA351.01954356E-746.00000430
TACGGAG351.01954356E-746.00000414
AGGCACG700.046.0000041
TATTACT700.046.00000422
TTCGGGT351.01954356E-746.00000413
AGGATAC351.01954356E-746.00000420
AATACGC351.01954356E-746.00000429
ATACCCG351.01954356E-746.00000430
TACGTTT351.01954356E-746.00000411
TCACGAC1200.046.025
TCGCCGT206.3113985E-446.025
TAGAGTC206.3113985E-446.024
CTAGACG206.3113985E-446.01
CGGGTCA453.092282E-1046.06
AGCGTAC206.3113985E-446.031
CAGCGAA301.8613682E-646.025
CCCCTTA253.41711E-546.016
Produced by FastQC (version 0.11.5)