##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544731_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1125674
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.731428459749445	33.0	31.0	34.0	31.0	34.0
2	33.104165149057366	34.0	33.0	34.0	31.0	34.0
3	33.13148744663197	34.0	33.0	34.0	31.0	34.0
4	36.45464850391854	37.0	37.0	37.0	35.0	37.0
5	36.452288140260855	37.0	37.0	37.0	35.0	37.0
6	36.51999602016214	37.0	37.0	37.0	35.0	37.0
7	36.6353899974593	37.0	37.0	37.0	35.0	37.0
8	36.63691885927897	37.0	37.0	37.0	35.0	37.0
9	38.361377272638435	39.0	39.0	39.0	37.0	39.0
10	38.25604748799386	39.0	39.0	39.0	37.0	39.0
11	38.052236260231645	39.0	39.0	39.0	35.0	39.0
12	36.969743460362416	39.0	35.0	39.0	35.0	39.0
13	36.84274310324304	39.0	35.0	39.0	35.0	39.0
14	37.82963717737107	40.0	35.0	41.0	35.0	41.0
15	37.891417053249874	40.0	35.0	41.0	35.0	41.0
16	37.962116918397335	40.0	35.0	41.0	35.0	41.0
17	37.919205738073366	39.0	35.0	41.0	35.0	41.0
18	37.96967772196924	39.0	36.0	41.0	35.0	41.0
19	37.90319310919502	39.0	36.0	41.0	35.0	41.0
20	37.72735445608586	39.0	35.0	41.0	35.0	41.0
21	37.6320080236374	39.0	35.0	41.0	35.0	41.0
22	37.55202571970215	38.0	35.0	41.0	35.0	41.0
23	37.472524016722424	38.0	35.0	41.0	35.0	41.0
24	37.436623747195014	38.0	35.0	41.0	35.0	41.0
25	37.41981692745857	38.0	35.0	41.0	35.0	41.0
26	37.34359859071099	38.0	35.0	41.0	35.0	41.0
27	37.32866265011007	38.0	35.0	41.0	35.0	41.0
28	37.36916016537648	38.0	35.0	41.0	35.0	41.0
29	37.353885760886364	38.0	35.0	41.0	35.0	41.0
30	37.30371759496977	37.0	35.0	41.0	35.0	41.0
31	37.128467033972534	37.0	35.0	41.0	35.0	41.0
32	36.97091253773295	37.0	35.0	41.0	34.0	41.0
33	36.796354006577396	37.0	35.0	41.0	34.0	41.0
34	36.66139841552705	37.0	35.0	41.0	34.0	41.0
35	36.52118108795264	37.0	35.0	41.0	34.0	41.0
36	36.43464893032974	37.0	35.0	41.0	33.0	41.0
37	36.39113366747389	37.0	35.0	41.0	33.0	41.0
38	36.31017417120765	37.0	35.0	41.0	33.0	41.0
39	36.33554563754693	36.0	35.0	41.0	33.0	41.0
40	36.17262013691353	36.0	35.0	41.0	32.0	41.0
41	36.208181942551754	36.0	35.0	41.0	33.0	41.0
42	36.214690043476175	36.0	35.0	41.0	33.0	41.0
43	36.15521722985518	36.0	35.0	41.0	33.0	41.0
44	36.057080469123385	36.0	35.0	41.0	33.0	41.0
45	35.96981275218225	35.0	35.0	40.0	33.0	41.0
46	35.94477797301883	35.0	35.0	40.0	33.0	41.0
47	35.89127047440022	35.0	35.0	40.0	33.0	41.0
48	35.860915327172876	35.0	35.0	40.0	33.0	41.0
49	35.852507031343	36.0	35.0	40.0	33.0	41.0
50	35.70793586775567	36.0	35.0	40.0	32.0	41.0
51	35.648227639618575	35.0	35.0	40.0	32.0	41.0
52	35.358546079948546	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	3.0
14	5.0
15	14.0
16	34.0
17	66.0
18	195.0
19	363.0
20	606.0
21	1048.0
22	1694.0
23	2712.0
24	4703.0
25	7483.0
26	9843.0
27	10728.0
28	10149.0
29	9921.0
30	10932.0
31	12730.0
32	16470.0
33	23082.0
34	65699.0
35	335572.0
36	47439.0
37	49074.0
38	88777.0
39	414468.0
40	1862.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.110006982483384	23.2911127022566	29.84887276422836	14.750007551031649
2	29.276238058265534	26.586827092035527	31.111760598539185	13.025174251159749
3	27.692209289723312	26.173830078690635	32.721729381685996	13.412231249900062
4	23.864102750885248	29.961427553625654	31.97417724847514	14.200292447013968
5	22.841160051666822	33.699099384013486	30.32325522309301	13.136485341226678
6	21.006170525391898	42.25806050419571	26.495148684254943	10.24062028615745
7	87.29454531240839	4.998072266037947	6.027766475906879	1.6796159456467858
8	88.1429259270446	3.6570090452475585	6.150448531279927	2.0496164964279178
9	83.33434013755314	5.797326757125065	8.108031277261444	2.7603018280603444
10	53.945636125556774	25.570724739134064	11.573510625634064	8.910128509675094
11	51.41159873995491	17.795560704075957	20.08787624125635	10.704964314712786
12	46.566412655884385	20.587665700726852	21.27205567508888	11.57386596829988
13	18.98498144222928	48.340105572306015	20.711502619763802	11.963410365700906
14	12.495624843427137	49.20687516989821	25.540698283872594	12.756801702802054
15	10.972981520404664	23.609677402160838	53.51691519924952	11.900425878184981
16	12.898228083796909	18.772841870736997	52.047750947432384	16.28117909803371
17	13.371811021663465	18.754719394780373	29.68452678128837	38.188942802267796
18	16.81854604441428	23.59386465353202	40.59070388051958	18.99688542153412
19	28.25604926470719	22.749126301220425	28.569194988957726	20.42562944511466
20	31.630294383631497	22.889220147218467	28.08424108578505	17.39624438336499
21	19.357735898670487	31.37302629358056	31.238440258902667	18.03079754884629
22	20.718254130414312	23.81151203634445	24.854442760515035	30.615791072726207
23	14.844351028805852	31.9405973665555	26.35132374026583	26.863727864372812
24	15.010207218075571	21.991269230700897	47.336084870042306	15.662438681181229
25	13.448120859147497	24.647811000342905	42.19463183834751	19.709436302162082
26	12.854698607234422	37.90306962761865	28.990009540950577	20.25222222419635
27	12.760976979125397	44.315849881937396	27.72596684297585	15.197206295961351
28	11.362525917805687	32.91192654356412	39.79562466575581	15.929922872874386
29	11.965009407697078	24.4537050691408	39.51739135842171	24.06389416474041
30	15.670256219829188	36.78702714995638	31.760083292320868	15.782633337893564
31	28.864395908584545	29.11979844963995	26.479868949624848	15.53593669215066
32	31.270421098826123	24.211627878053505	30.563555700851225	13.954395322269148
33	30.34804037403369	27.712552657341288	24.89210908309155	17.047297885533467
34	18.80615524565727	27.59955368961173	27.052947833919944	26.541343230811055
35	18.423184687573844	25.90279246033932	32.98299507672737	22.691027775359473
36	34.70303125061074	23.295909828245122	26.336399348301555	15.664659572842583
37	16.41612047537742	37.02643927105005	31.46044058937135	15.09699966420118
38	17.954310040029352	36.55694277384038	24.65474018232632	20.834007003803944
39	19.919887995991733	34.006115447278695	29.68577048061872	16.388226076110847
40	25.38647956690836	24.32311663945334	27.013327126681435	23.277076666956866
41	13.948265661283818	25.303595890106727	30.095125231639003	30.653013216970454
42	19.376213717292927	21.7020203007265	28.54254428902151	30.379221692959064
43	19.20111861871199	22.534588166733887	30.911791513351112	27.352501701203014
44	14.763332900999757	29.676620406973953	32.98868055938043	22.571366132645863
45	14.107725682568843	43.606585920968236	23.963598697313788	18.32208969914913
46	22.048923578229576	35.489848748394294	25.87933984439545	16.581887828980683
47	18.730023079506143	29.395899700979143	26.55378022411462	25.32029699540009
48	20.367353247920803	25.47433804103142	35.51721013366214	18.641098577385637
49	22.97672327867571	23.060850654807695	35.90879775139161	18.053628315124985
50	19.655424216958018	37.504197485239956	25.796189660594454	17.044188637207576
51	17.377144715077367	34.77454396210626	24.679347661934095	23.16896366088228
52	17.259703964025107	27.47083080891981	37.929631491888415	17.339833735166664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9145.0
1	7319.5
2	5494.0
3	8200.5
4	10907.0
5	7226.5
6	3546.0
7	3815.5
8	4085.0
9	4541.0
10	4997.0
11	5447.5
12	5898.0
13	5848.5
14	5547.5
15	5296.0
16	4892.0
17	4488.0
18	4183.5
19	3879.0
20	4300.0
21	4721.0
22	4508.0
23	4295.0
24	5285.5
25	6276.0
26	6839.0
27	7402.0
28	10972.5
29	14543.0
30	15647.5
31	16752.0
32	20392.0
33	24032.0
34	25502.0
35	26972.0
36	28570.5
37	30169.0
38	36397.0
39	61216.5
40	79808.0
41	104228.0
42	128648.0
43	139266.5
44	149885.0
45	144535.5
46	139186.0
47	122553.5
48	105921.0
49	95645.5
50	85370.0
51	70800.0
52	56230.0
53	50722.0
54	45214.0
55	38287.0
56	31360.0
57	25068.5
58	18777.0
59	16467.5
60	14158.0
61	12081.0
62	10004.0
63	8462.0
64	5852.0
65	4784.0
66	4117.0
67	3450.0
68	2502.0
69	1554.0
70	1385.5
71	1217.0
72	1089.0
73	961.0
74	710.5
75	460.0
76	335.5
77	211.0
78	168.5
79	126.0
80	84.0
81	42.0
82	41.0
83	40.0
84	29.0
85	18.0
86	12.0
87	6.0
88	4.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1125674.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.482010027422794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14716163954749	11.479471000084128
2	10.959564709519185	3.3935218145793
3	4.144688160798367	1.9250431099806251
4	2.0651341336235354	1.2788970945893061
5	1.1924188697566027	0.9230520449229938
6	0.7989651235667732	0.7421751632773126
7	0.5354965115591748	0.5803393653126347
8	0.4157178566861162	0.5148918420634531
9	0.3192263017062967	0.4448038323630581
>10	2.996606962428809	10.787124729374378
>50	0.9936525555723275	10.918615330331685
>100	1.3933050343950915	51.14392276478259
>500	0.03287184890750009	3.400136634309356
>1k	0.004036893725482467	1.1549857544621034
>5k	0.001153398207280705	1.3130195195670757
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8287	0.7361811679047398	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6419	0.5702361429685682	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT	2955	0.2625093943717275	Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2739	0.243320890417652	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	2690	0.2389679427614034	No Hit
TTTTGAGGGATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCAT	1286	0.11424266705991255	No Hit
TTCTGCGGGAGGAAATTATTGAAAGTTTGGGAGGGACTATTCACAGTATAGA	1171	0.10402656541769642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27254782468103556	0.0
2	0.0	0.0	0.0	0.7472856262114964	0.0
3	0.0	0.0	0.0	0.991583708960143	0.0
4	0.0	0.0	0.0	1.2520498830034272	0.0
5	0.0	0.0	0.0	1.9041925104426325	0.0
6	0.0	0.0	0.0	2.349525706376802	0.0
7	0.0	0.0	0.0	2.6704001336088425	0.0
8	0.0	0.0	0.0	3.38001943724382	0.0
9	0.0	0.0	0.0	3.637731705627029	0.0
10	0.0	0.0	0.0	4.141874112753781	0.0
11	0.0	0.0	0.0	4.765411655594781	0.0
12	0.0	0.0	0.0	5.261381181407761	0.0
13	8.88356664540533E-5	0.0	0.0	5.4552206056105055	0.0
14	1.776713329081066E-4	0.0	0.0	5.518293928792883	0.0
15	1.776713329081066E-4	0.0	0.0	5.634224473515423	0.0
16	1.776713329081066E-4	0.0	0.0	5.908282504526177	0.0
17	1.776713329081066E-4	0.0	0.0	6.222494256774164	0.0
18	1.776713329081066E-4	0.0	0.0	6.68923684832376	0.0
19	1.776713329081066E-4	0.0	0.0	6.887518055849207	0.0
20	1.776713329081066E-4	0.0	0.0	7.128706890271961	0.0
21	2.6650699936215993E-4	0.0	0.0	7.422841781901332	0.0
22	8.88356664540533E-4	0.0	0.0	7.710580505546011	0.0
23	8.88356664540533E-4	0.0	0.0	8.055707069720007	0.0
24	0.0021320559948972795	0.0	0.0	8.309244061779875	0.0
25	0.0021320559948972795	0.0	0.0	8.505304377643972	0.0
26	0.0021320559948972795	0.0	0.0	8.699499144512533	0.0
27	0.0021320559948972795	0.0	0.0	8.882145274742065	0.0
28	0.0021320559948972795	0.0	0.0	9.063814212640604	0.0
29	0.0021320559948972795	0.0	0.0	9.287946599104181	0.0
30	0.0021320559948972795	0.0	0.0	9.560405588118762	0.0
31	0.0021320559948972795	0.0	0.0	9.81918388449942	0.0
32	0.0021320559948972795	0.0	0.0	10.03638708897958	0.0
33	0.0021320559948972795	0.0	0.0	10.253590293459741	0.0
34	0.0021320559948972795	0.0	0.0	10.482963984244106	0.0
35	0.0021320559948972795	0.0	0.0	10.812188964122829	0.0
36	0.0021320559948972795	0.0	0.0	11.036054843587042	0.0
37	0.0021320559948972795	0.0	0.0	11.289769506979818	0.0
38	0.0021320559948972795	0.0	0.0	11.506883875793525	0.0
39	0.0021320559948972795	0.0	0.0	11.749227573880182	0.0
40	0.0021320559948972795	0.0	0.0	12.020442863564407	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	35	1.01954356E-7	46.000004	8
CGTATTA	35	1.01954356E-7	46.000004	42
CGACCCC	35	1.01954356E-7	46.000004	20
CACGAGA	35	1.01954356E-7	46.000004	30
TACGGAG	35	1.01954356E-7	46.000004	14
AGGCACG	70	0.0	46.000004	1
TATTACT	70	0.0	46.000004	22
TTCGGGT	35	1.01954356E-7	46.000004	13
AGGATAC	35	1.01954356E-7	46.000004	20
AATACGC	35	1.01954356E-7	46.000004	29
ATACCCG	35	1.01954356E-7	46.000004	30
TACGTTT	35	1.01954356E-7	46.000004	11
TCACGAC	120	0.0	46.0	25
TCGCCGT	20	6.3113985E-4	46.0	25
TAGAGTC	20	6.3113985E-4	46.0	24
CTAGACG	20	6.3113985E-4	46.0	1
CGGGTCA	45	3.092282E-10	46.0	6
AGCGTAC	20	6.3113985E-4	46.0	31
CAGCGAA	30	1.8613682E-6	46.0	25
CCCCTTA	25	3.41711E-5	46.0	16
>>END_MODULE