FastQCFastQC Report
Thu 26 May 2016
SRR1544730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544730_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1677149
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54460.324717720369508No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT43880.26163447612585405TruSeq Adapter, Index 27 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43200.25757997649582715No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT34200.20391748139252983TruSeq Adapter, Index 27 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT26370.15723111065266115TruSeq Adapter, Index 27 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG253.4178316E-546.038
AACCGTA405.6134013E-946.030
TCGCAAG551.8189894E-1246.01
GTCGATA206.3122885E-446.09
TCCGCAT405.6134013E-946.013
GCGGTAT206.3122885E-446.09
CGGCATA206.3122885E-446.033
CGTGAAT301.8619176E-646.030
CCGGATA650.046.020
TATATCG453.110472E-1046.039
TTCGTAA301.8619176E-646.041
ATTCGAC650.046.028
ACGGGTC800.046.05
AATATCG301.8619176E-646.022
ACGATTT206.3122885E-446.045
TACGCTA206.3122885E-446.010
ATATCGT301.8619176E-646.023
TCGTGTA206.3122885E-446.026
TACGCGA301.8619176E-646.021
CCGTAAC206.3122885E-446.019
Produced by FastQC (version 0.11.5)