##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544730_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1677149
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.598546104132666	33.0	31.0	34.0	31.0	34.0
2	32.99908475633352	34.0	33.0	34.0	31.0	34.0
3	33.04618253953584	34.0	33.0	34.0	31.0	34.0
4	36.37977782534527	37.0	37.0	37.0	35.0	37.0
5	36.37917561290023	37.0	37.0	37.0	35.0	37.0
6	36.45891331062416	37.0	37.0	37.0	35.0	37.0
7	36.578828118431936	37.0	37.0	37.0	35.0	37.0
8	36.566968707014105	37.0	37.0	37.0	35.0	37.0
9	38.29048402974333	39.0	39.0	39.0	37.0	39.0
10	38.15053582001361	39.0	39.0	39.0	37.0	39.0
11	37.92733800038041	39.0	38.0	39.0	35.0	39.0
12	37.13392906652897	39.0	35.0	39.0	35.0	39.0
13	36.99515964294168	39.0	35.0	39.0	35.0	39.0
14	38.0700569836073	40.0	35.0	41.0	35.0	41.0
15	38.12810728206021	40.0	35.0	41.0	35.0	41.0
16	38.20585827496543	40.0	35.0	41.0	35.0	41.0
17	38.179177878650016	40.0	35.0	41.0	35.0	41.0
18	38.243876960246226	40.0	36.0	41.0	35.0	41.0
19	38.21256906810307	40.0	36.0	41.0	35.0	41.0
20	38.08877326939944	40.0	35.0	41.0	35.0	41.0
21	37.999599916286506	40.0	35.0	41.0	35.0	41.0
22	37.91735141004168	40.0	35.0	41.0	35.0	41.0
23	37.86147265389062	40.0	35.0	41.0	35.0	41.0
24	37.82077501760428	40.0	35.0	41.0	35.0	41.0
25	37.830772340442024	40.0	35.0	41.0	35.0	41.0
26	37.76859956986529	40.0	35.0	41.0	35.0	41.0
27	37.735818344106576	40.0	35.0	41.0	35.0	41.0
28	37.723524862728354	39.0	35.0	41.0	35.0	41.0
29	37.71728928079735	39.0	35.0	41.0	35.0	41.0
30	37.67687963323473	39.0	35.0	41.0	35.0	41.0
31	37.56509469343511	39.0	35.0	41.0	34.0	41.0
32	37.453731302347016	39.0	35.0	41.0	34.0	41.0
33	37.34763339452845	39.0	35.0	41.0	34.0	41.0
34	37.226071744370955	39.0	35.0	41.0	34.0	41.0
35	37.08688554207169	39.0	35.0	41.0	34.0	41.0
36	37.03496290430964	39.0	35.0	41.0	34.0	41.0
37	36.98475806264083	39.0	35.0	41.0	33.0	41.0
38	36.86895737945764	39.0	35.0	41.0	33.0	41.0
39	36.88623372163117	39.0	35.0	41.0	33.0	41.0
40	36.795272811181356	39.0	35.0	41.0	33.0	41.0
41	36.79252886893174	39.0	35.0	41.0	33.0	41.0
42	36.79269999266612	39.0	35.0	41.0	33.0	41.0
43	36.70478770818812	38.0	35.0	41.0	33.0	41.0
44	36.61651886624265	38.0	35.0	41.0	33.0	41.0
45	36.53720510223003	38.0	35.0	41.0	33.0	41.0
46	36.48459021828114	38.0	35.0	41.0	33.0	41.0
47	36.41574481456328	38.0	35.0	41.0	33.0	41.0
48	36.38879073952285	38.0	35.0	41.0	33.0	41.0
49	36.39110657431152	38.0	35.0	41.0	33.0	41.0
50	36.25782503522347	38.0	35.0	40.0	33.0	41.0
51	36.19924467056892	37.0	35.0	40.0	32.0	41.0
52	35.95282053055513	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	1.0
14	14.0
15	17.0
16	48.0
17	121.0
18	256.0
19	463.0
20	858.0
21	1344.0
22	2087.0
23	3379.0
24	5639.0
25	9062.0
26	12393.0
27	14014.0
28	14089.0
29	14863.0
30	16678.0
31	20247.0
32	26085.0
33	36384.0
34	91765.0
35	384377.0
36	69404.0
37	85596.0
38	160867.0
39	704897.0
40	2195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.529042440474875	24.737694742685353	28.29623366796868	13.43702914887109
2	29.911713270556163	25.670348907580664	29.77147528335288	14.646462538510294
3	28.70007375611827	24.659466749823657	32.88491362425163	13.75554586980644
4	24.516307137886972	28.53050027159185	32.175316564002365	14.777876026518813
5	22.152831978554083	33.2756362135982	29.930316268858643	14.641215538989083
6	21.89471537710722	41.47001846586081	25.409072181422165	11.2261939756098
7	87.49592314099701	4.3036128572953265	5.893215212244112	2.307248789463548
8	87.1905239188647	3.411145938732933	6.239576805638617	3.158753336763758
9	83.23082803018694	4.871779430450126	8.250191247170049	3.6472012921928822
10	49.55594285302021	27.491296241419217	12.73136733826273	10.221393567297838
11	44.00259010976365	19.937226805728052	22.898859910479032	13.16132317402926
12	41.019193881998554	20.945246963746214	24.79433848751661	13.241220666738615
13	20.571577122843586	39.86360186244633	25.877665013663066	13.687156001047015
14	14.214717952906986	43.402345289535994	27.004100410875836	15.378836346681183
15	13.249747040960584	24.422278521467085	47.99472199548162	14.333252442090716
16	15.302277853667146	21.07218857716279	46.88581634666926	16.7397172225008
17	15.014468004929796	20.60514599478043	28.817415745410813	35.56297025487896
18	18.65517017271572	23.30013612386258	38.334280376996915	19.710413326424785
19	26.436053087710153	24.27995365945423	28.175910428948175	21.108082823887443
20	29.420284065399077	23.6145983451679	27.804804462811596	19.16031312662143
21	20.86463397110215	29.19943308555173	31.18041390478723	18.755519038558887
22	20.302310647414153	23.931862941217506	27.285172635227994	28.48065377614034
23	16.063867909172053	30.87024468309017	27.230556140211753	25.835331267526023
24	16.424420251271652	23.239795629368647	41.83003418300938	18.50574993635032
25	15.098419997269175	25.36107406080199	38.2639825084116	21.276523433517237
26	15.1377128686837	34.1109227623783	28.64122388648832	22.11014048244968
27	15.573631203906151	37.886317792873506	27.986362571244417	18.55368843197593
28	13.083751056107717	30.741514319836817	37.49583370350518	18.67890092055029
29	14.25723057402771	24.433189895471422	36.51261754322365	24.796961987277218
30	16.59709423551515	33.36084033082332	30.99879617135985	19.043269262301678
31	26.557151451659934	28.58791914135238	25.994410753010015	18.860518653977675
32	27.719719595575587	24.526860761923956	28.96939985654226	18.784019785958193
33	26.647781443389945	27.47281249310586	26.231897106339392	19.64750895716481
34	18.676158170800566	26.989551912203385	27.82334783611951	26.510942080876536
35	16.338560259106377	25.62974428628583	33.18411184694979	24.847583607657995
36	29.033496725693425	23.782204204873867	27.8471978339432	19.337101235489513
37	17.751255255197957	33.45790982196573	30.259446238825532	18.531388684010782
38	18.149967593815457	32.614812398898366	25.979981504326688	23.255238502959486
39	18.23988208561076	31.664866985580886	29.912488395485433	20.18276253332292
40	23.907357068453667	24.630667877451558	28.650346510655883	22.811628543438893
41	15.88934555009722	24.504918763926163	29.922088019609465	29.68364766636715
42	19.905327433638874	22.368793708847573	28.01420744370357	29.71167141380998
43	20.385368264835147	23.321779996887575	28.933624859806734	27.35922687847055
44	16.373321630934402	28.647305635933364	30.516489590370327	24.462883142761914
45	15.542029956789765	37.50710282747687	25.50894404730886	21.441923168424513
46	21.363277800600898	32.632819147255255	27.247787763639366	18.75611528850448
47	18.05897985211809	28.241796047936113	28.231659798861042	25.467564301084757
48	19.252672243193658	24.781399863697263	33.941468527840996	22.024459365268083
49	20.582667371831604	23.727885834830417	34.952708435565356	20.736738357772623
50	19.569757964259583	31.602558866266506	28.49251915005763	20.335164019416283
51	17.43887990870221	31.369186637561718	26.22712710677465	24.96480634696142
52	17.502619027886013	25.983082004043766	36.302260562418724	20.212038405651494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5254.0
1	4680.0
2	4106.0
3	7128.5
4	10151.0
5	6962.0
6	3773.0
7	4072.5
8	4372.0
9	4794.0
10	5216.0
11	5442.5
12	5669.0
13	5567.5
14	5455.0
15	5444.0
16	5866.0
17	6288.0
18	5571.5
19	4855.0
20	4901.0
21	4947.0
22	6912.5
23	8878.0
24	9437.5
25	9997.0
26	10215.5
27	10434.0
28	16233.5
29	22033.0
30	23000.5
31	23968.0
32	29530.0
33	35092.0
34	37930.5
35	40769.0
36	47475.5
37	54182.0
38	62835.5
39	90963.5
40	110438.0
41	136429.0
42	162420.0
43	177774.0
44	193128.0
45	188957.5
46	184787.0
47	179679.0
48	174571.0
49	164813.0
50	155055.0
51	131154.5
52	107254.0
53	90102.0
54	72950.0
55	61554.0
56	50158.0
57	43004.0
58	35850.0
59	31643.5
60	27437.0
61	23314.0
62	19191.0
63	17259.5
64	12150.0
65	8972.0
66	7602.5
67	6233.0
68	5396.0
69	4559.0
70	3578.0
71	2597.0
72	1988.5
73	1380.0
74	1254.5
75	1129.0
76	882.5
77	636.0
78	467.5
79	299.0
80	268.5
81	238.0
82	146.0
83	54.0
84	64.5
85	75.0
86	45.5
87	16.0
88	11.0
89	5.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1677149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.84452948471179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28691466072497	12.176390653614266
2	11.468947370718087	3.8637804428933706
3	4.513180899744016	2.280672262067285
4	2.27058651505561	1.5298784600177284
5	1.3897852767847154	1.1705139536109237
6	0.9119033236798024	0.9216349453758664
7	0.6281145257866908	0.7406205554572771
8	0.4714355609844045	0.6352888165714755
9	0.372096057032847	0.5641004703451311
>10	3.422810464184934	13.316228638432232
>50	0.9823529220819878	11.754329494037048
>100	1.2572690303979466	47.24639477512796
>500	0.021394254629573426	2.260279231059556
>1k	0.0028525672839431232	1.2127858799307651
>5k	3.565709104928904E-4	0.32710142145913956
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5446	0.324717720369508	No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT	4388	0.26163447612585405	TruSeq Adapter, Index 27 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4320	0.25757997649582715	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	3420	0.20391748139252983	TruSeq Adapter, Index 27 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2637	0.15723111065266115	TruSeq Adapter, Index 27 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2754674748635929	0.0
2	0.0	0.0	0.0	0.8380292985298265	0.0
3	0.0	0.0	0.0	1.1286415220114612	0.0
4	0.0	0.0	0.0	1.5048156126855754	0.0
5	0.0	0.0	0.0	2.499181646949675	0.0
6	0.0	0.0	0.0	3.1767600851206423	0.0
7	0.0	0.0	0.0	3.653163791648804	0.0
8	0.0	0.0	0.0	4.704233195738721	0.0
9	0.0	0.0	0.0	5.037358040341079	0.0
10	0.0	0.0	0.0	5.719825728065902	0.0
11	0.0	0.0	0.0	6.580333649544555	0.0
12	0.0	0.0	0.0	7.26572296200278	0.0
13	0.0	0.0	0.0	7.529980937889239	0.0
14	0.0	0.0	0.0	7.615542805081719	0.0
15	0.0	0.0	0.0	7.785533664570053	0.0
16	0.0	0.0	0.0	8.153181381022199	0.0
17	0.0	0.0	0.0	8.590649966103191	0.0
18	0.0	0.0	0.0	9.217904908866178	0.0
19	0.0	0.0	0.0	9.516626131607865	0.0
20	5.9624994559219245E-5	0.0	0.0	9.805509230247283	0.0
21	5.9624994559219245E-5	0.0	0.0	10.21429819294529	0.0
22	5.9624994559219245E-5	0.0	0.0	10.614024156470295	0.0
23	5.9624994559219245E-5	0.0	0.0	11.090129738025661	0.0
24	1.7887498367765773E-4	0.0	0.0	11.441320955979462	0.0
25	1.7887498367765773E-4	0.0	0.0	11.725791805021498	0.0
26	1.7887498367765773E-4	0.0	0.0	12.02952152730616	0.0
27	2.3849997823687698E-4	0.0	0.0	12.291156003432015	0.0
28	3.5774996735531546E-4	0.0	0.0	12.57634235240876	0.0
29	3.5774996735531546E-4	0.0	0.0	12.876136825052514	0.0
30	3.5774996735531546E-4	0.0	0.0	13.248256416096602	0.0
31	3.5774996735531546E-4	0.0	0.0	13.599626509034081	0.0
32	3.5774996735531546E-4	0.0	0.0	13.900017231623428	0.0
33	3.5774996735531546E-4	0.0	0.0	14.20523757877207	0.0
34	3.5774996735531546E-4	0.0	0.0	14.511769675801018	0.0
35	3.5774996735531546E-4	0.0	0.0	14.897603015593726	0.0
36	4.173749619145347E-4	0.0	0.0	15.215225361610687	0.0
37	4.7699995647375396E-4	0.0	0.0	15.516689334102098	0.0
38	4.7699995647375396E-4	0.0	0.0	15.829839805527119	0.0
39	4.7699995647375396E-4	0.0	0.0	16.128322528290568	0.0
40	4.7699995647375396E-4	0.0	0.0	16.462878372762347	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	25	3.4178316E-5	46.0	38
AACCGTA	40	5.6134013E-9	46.0	30
TCGCAAG	55	1.8189894E-12	46.0	1
GTCGATA	20	6.3122885E-4	46.0	9
TCCGCAT	40	5.6134013E-9	46.0	13
GCGGTAT	20	6.3122885E-4	46.0	9
CGGCATA	20	6.3122885E-4	46.0	33
CGTGAAT	30	1.8619176E-6	46.0	30
CCGGATA	65	0.0	46.0	20
TATATCG	45	3.110472E-10	46.0	39
TTCGTAA	30	1.8619176E-6	46.0	41
ATTCGAC	65	0.0	46.0	28
ACGGGTC	80	0.0	46.0	5
AATATCG	30	1.8619176E-6	46.0	22
ACGATTT	20	6.3122885E-4	46.0	45
TACGCTA	20	6.3122885E-4	46.0	10
ATATCGT	30	1.8619176E-6	46.0	23
TCGTGTA	20	6.3122885E-4	46.0	26
TACGCGA	30	1.8619176E-6	46.0	21
CCGTAAC	20	6.3122885E-4	46.0	19
>>END_MODULE