FastQCFastQC Report
Thu 26 May 2016
SRR1544729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544729_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1356439
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60140.44336678612160224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55800.41137124485509485No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19770.145749274386832No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT19630.14471716015242853Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT19040.14036753587887107Illumina Single End Adapter 2 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGT351.01974365E-746.00000446
GCGTTGC351.01974365E-746.00000426
TCGGCCA351.01974365E-746.00000410
AATGCGT351.01974365E-746.00000423
TCTACGG351.01974365E-746.0000042
GAACGAC351.01974365E-746.00000422
CGTCAAC351.01974365E-746.00000432
GTACGTC351.01974365E-746.00000422
CGAAGTA700.046.00000435
ACGGTCT1400.046.00000430
CGGACAC253.417484E-546.043
CTTCGTA206.3118583E-446.013
TCGCCCT206.3118583E-446.016
ACTATCG301.8616538E-646.028
GTACCGG253.417484E-546.02
GTACCCG206.3118583E-446.021
CAAACGG301.8616538E-646.02
CGAACGG253.417484E-546.02
CGAACCC405.6134013E-946.045
ACACGAT206.3118583E-446.018
Produced by FastQC (version 0.11.5)