##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544729_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1356439
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.716835773669146	33.0	31.0	34.0	31.0	34.0
2	33.12732824697609	34.0	33.0	34.0	31.0	34.0
3	33.15783385762279	34.0	33.0	34.0	31.0	34.0
4	36.46311555477246	37.0	37.0	37.0	35.0	37.0
5	36.46600326295543	37.0	37.0	37.0	35.0	37.0
6	36.528806676894426	37.0	37.0	37.0	35.0	37.0
7	36.6548182409972	37.0	37.0	37.0	35.0	37.0
8	36.66223103287358	37.0	37.0	37.0	35.0	37.0
9	38.418791408976006	39.0	39.0	39.0	37.0	39.0
10	38.262690028818106	39.0	39.0	39.0	37.0	39.0
11	38.05106458897156	39.0	39.0	39.0	35.0	39.0
12	37.314062777611085	39.0	35.0	39.0	35.0	39.0
13	37.200245643187785	39.0	35.0	39.0	35.0	39.0
14	38.349793097957225	40.0	35.0	41.0	35.0	41.0
15	38.393152216944515	40.0	35.0	41.0	35.0	41.0
16	38.47250631985663	40.0	35.0	41.0	35.0	41.0
17	38.434798026302694	40.0	35.0	41.0	35.0	41.0
18	38.442967210468	40.0	36.0	41.0	35.0	41.0
19	38.414920243372535	40.0	36.0	41.0	35.0	41.0
20	38.280219014640544	40.0	35.0	41.0	35.0	41.0
21	38.20261139645793	40.0	35.0	41.0	35.0	41.0
22	38.14802877239596	40.0	35.0	41.0	35.0	41.0
23	38.0848125127632	40.0	35.0	41.0	35.0	41.0
24	38.03373539097593	40.0	35.0	41.0	35.0	41.0
25	38.016019887366845	40.0	35.0	41.0	35.0	41.0
26	37.94398937217228	40.0	35.0	41.0	35.0	41.0
27	37.910326966417216	40.0	35.0	41.0	35.0	41.0
28	37.89320271681955	40.0	35.0	41.0	35.0	41.0
29	37.900524830088195	40.0	35.0	41.0	35.0	41.0
30	37.85782773865983	40.0	35.0	41.0	35.0	41.0
31	37.75619618722257	40.0	35.0	41.0	35.0	41.0
32	37.63542997510393	40.0	35.0	41.0	35.0	41.0
33	37.48318649050934	40.0	35.0	41.0	35.0	41.0
34	37.349727484980896	40.0	35.0	41.0	34.0	41.0
35	37.22051489230257	40.0	35.0	41.0	34.0	41.0
36	37.13955806342932	40.0	35.0	41.0	34.0	41.0
37	37.09147775904408	40.0	35.0	41.0	34.0	41.0
38	36.9832539465468	40.0	35.0	41.0	33.0	41.0
39	37.02413672859598	39.0	35.0	41.0	33.0	41.0
40	36.89273605374071	39.0	35.0	41.0	33.0	41.0
41	36.89477521657811	39.0	35.0	41.0	33.0	41.0
42	36.89703702120036	39.0	35.0	41.0	33.0	41.0
43	36.83089987828424	39.0	35.0	41.0	33.0	41.0
44	36.75645863912789	39.0	35.0	41.0	33.0	41.0
45	36.693164970927555	39.0	35.0	41.0	33.0	41.0
46	36.65699600203179	39.0	35.0	41.0	33.0	41.0
47	36.58713366395393	39.0	35.0	41.0	33.0	41.0
48	36.54631059708546	38.0	35.0	41.0	33.0	41.0
49	36.53149828337286	38.0	35.0	41.0	33.0	41.0
50	36.41947702771743	38.0	35.0	41.0	33.0	41.0
51	36.33512675468635	38.0	35.0	40.0	33.0	41.0
52	36.05372375757406	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	5.0
14	15.0
15	17.0
16	52.0
17	111.0
18	212.0
19	320.0
20	567.0
21	965.0
22	1555.0
23	2573.0
24	4651.0
25	7775.0
26	10408.0
27	11754.0
28	11081.0
29	11226.0
30	12220.0
31	14550.0
32	18010.0
33	25028.0
34	67451.0
35	296482.0
36	52132.0
37	61591.0
38	122825.0
39	620498.0
40	2361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.36540677465039	23.907009456378063	28.608363516531156	15.119220252440396
2	29.544196237353837	25.92545628664466	30.49035010052055	14.039997375480947
3	27.58863465294053	25.4645435585382	33.95287218960823	12.993949598913035
4	24.540727596301785	29.574422439932796	32.19164297104404	13.693206992721382
5	22.812378588347872	34.1647504974422	29.554959714369755	13.467911199840168
6	21.38231059413656	43.0019337397406	25.48290044742152	10.13285521870132
7	89.63565630301105	3.6439530270067433	5.261054864981028	1.4593358050011833
8	90.27173356118485	2.6798846096285933	5.464528814049139	1.58385301513743
9	86.27605074758246	4.545652255648798	6.809889718594055	2.368407278174691
10	46.986631908991114	33.69167356585884	10.814566670524808	8.507127854625237
11	42.02865001669813	20.05574891314685	25.179974919624105	12.735626150530912
12	39.58113855470095	21.066262471073156	25.720581611115573	13.632017363110322
13	20.50302298887012	39.85907217353674	25.93813654723876	13.699768290354378
14	13.402740558182122	43.170684417065566	28.348786786578685	15.077788238173628
15	12.151818106085125	25.99416560567781	48.051110296887664	13.802905991349409
16	15.576299413390501	21.204786945819162	47.36969373484543	15.849219905944903
17	15.617657705211958	21.364396039925126	30.28178930272574	32.73615695213717
18	17.739021069137646	24.844537793442978	38.23828421329673	19.178156924122646
19	25.42126848313857	24.22106707341797	30.179757438410427	20.17790700503303
20	28.648984583899463	23.796941845523463	29.888627501863336	17.66544606871374
21	19.87313841610275	29.724152726366608	32.48793347876314	17.914775378767494
22	20.536345534152293	23.99400194184921	27.81695306607964	27.65269945791886
23	15.963121083955858	29.691198793311013	28.200383504160527	26.1452966185726
24	15.959066349463559	24.04523904134281	43.584709669951984	16.410984939241647
25	15.396490369268356	26.267380988013468	38.40305387857471	19.933074764143466
26	15.66439773554137	35.561201056590086	30.098810193455066	18.675591014413477
27	14.999568723694908	38.87288702256423	29.597792455097498	16.52975179864336
28	13.17663381840245	31.81285704701796	38.43062607312234	16.57988306145724
29	14.223345096978191	24.998470259259722	37.97914981801615	22.79903482574594
30	16.414965951288632	32.89783027471195	33.04837150804422	17.638832265955195
31	25.575790728517834	28.785444830176658	29.573095435917136	16.065669005388372
32	28.03517150421066	25.927004457996265	30.391783191135026	15.646040846658051
33	26.963836928899866	28.152537637151394	26.707725153877178	18.17590028007157
34	19.575299737032037	28.18158428060532	28.517684908794276	23.725431073568366
35	18.751746300423388	27.278410603056972	32.324195927719565	21.64564716880007
36	29.469589122695528	25.145325370326272	28.722559584323363	16.662525922654833
37	18.405988031898225	34.17492419489561	31.023953159707148	16.39513461349902
38	18.27380368744927	34.766104483872844	27.089238808379886	19.870853020298
39	19.962490019823967	33.05338463432561	29.22564155114974	17.758483794700684
40	23.53913445425854	25.956862048348654	29.04597995191822	21.458023545474582
41	16.375155830818784	26.88657580621023	30.09740946699409	26.640858895976894
42	20.757881482322464	24.115201641946303	28.71231216442464	26.414604711306588
43	20.15638005100119	25.31046364783083	30.10728827466624	24.425868026501746
44	16.94576755755327	30.59599436465628	31.553206594620175	20.905031483170273
45	16.231470784900758	39.15775055125958	25.08752697319968	19.523251690639977
46	19.982173912722946	34.428455684332285	28.173179921839463	17.416190481105307
47	19.84335454819568	29.253213745697376	28.42538440726048	22.478047298846466
48	20.831382760301054	26.30225170464724	33.611094933130055	19.255270601921648
49	22.113489806766097	25.517623719164668	34.116683463097125	18.252203010972114
50	18.951239237444515	33.73775009418043	28.63748388243039	18.67352678594467
51	18.211287053822545	33.09702832195182	26.61107502806982	22.080609596155817
52	18.511484851143326	28.070484555516316	35.49492457825232	17.923106015088035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6270.0
1	5452.0
2	4634.0
3	7725.0
4	10816.0
5	7303.5
6	3791.0
7	4058.0
8	4325.0
9	4499.5
10	4674.0
11	4720.5
12	4767.0
13	4653.0
14	4953.5
15	5368.0
16	4728.0
17	4088.0
18	4595.0
19	5102.0
20	5306.5
21	5511.0
22	6998.5
23	8486.0
24	9070.0
25	9654.0
26	12538.5
27	15423.0
28	19328.0
29	23233.0
30	26443.0
31	29653.0
32	30394.5
33	31136.0
34	38912.5
35	46689.0
36	53892.0
37	61095.0
38	65107.0
39	82097.5
40	95076.0
41	116565.0
42	138054.0
43	150793.0
44	163532.0
45	152178.0
46	140824.0
47	127826.0
48	114828.0
49	106369.0
50	97910.0
51	89371.5
52	80833.0
53	69281.5
54	57730.0
55	48038.5
56	38347.0
57	30973.0
58	23599.0
59	20466.0
60	17333.0
61	13989.0
62	10645.0
63	9096.0
64	6005.0
65	4463.0
66	3560.0
67	2657.0
68	2171.5
69	1686.0
70	1489.0
71	1292.0
72	956.5
73	621.0
74	580.5
75	540.0
76	388.5
77	237.0
78	186.0
79	135.0
80	125.0
81	115.0
82	81.5
83	48.0
84	26.0
85	4.0
86	2.5
87	1.0
88	1.0
89	2.5
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1356439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.963252795996778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79299174202805	11.34109695600862
2	10.014938150169634	2.997121025545211
3	3.5163505946198805	1.5784812859995259
4	1.694729282840982	1.0143465071971176
5	1.0076924970731598	0.7539178787167466
6	0.6671937148474764	0.5990032931497787
7	0.473489518078676	0.49594603486861405
8	0.3726522036780589	0.44608713028960606
9	0.2824173382670499	0.38032938238161623
>10	3.3577417615651752	12.094439687994821
>50	1.1148404837240007	11.86279610749959
>100	1.6811794494550625	53.212578037126605
>500	0.021305840355604972	1.930992504060695
>1k	0.0014864539782980214	0.4332777958813861
>5k	9.909693188653477E-4	0.8595863732800805
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6014	0.44336678612160224	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5580	0.41137124485509485	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1977	0.145749274386832	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT	1963	0.14471716015242853	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	1904	0.14036753587887107	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15149962512136558	0.0
2	0.0	0.0	0.0	0.4358470966995198	0.0
3	0.0	0.0	0.0	0.5835868771098442	0.0
4	0.0	0.0	0.0	0.7617003049897563	0.0
5	0.0	0.0	0.0	1.2262254329166296	0.0
6	0.0	0.0	0.0	1.5464757353629615	0.0
7	0.0	0.0	0.0	1.7615978307907691	0.0
8	0.0	0.0	0.0	2.2065127882639763	0.0
9	0.0	0.0	0.0	2.3669328292684004	0.0
10	0.0	0.0	0.0	2.7018538983323244	0.0
11	0.0	0.0	0.0	3.1692542016264644	0.0
12	0.0	0.0	0.0	3.542289774918002	0.0
13	0.0	0.0	0.0	3.683394535250019	0.0
14	7.372244531453313E-5	0.0	0.0	3.728512671782513	0.0
15	7.372244531453313E-5	0.0	0.0	3.8163161041521216	0.0
16	7.372244531453313E-5	0.0	0.0	4.019937498110862	0.0
17	7.372244531453313E-5	0.0	0.0	4.262115730969104	0.0
18	7.372244531453313E-5	0.0	0.0	4.600059420290924	0.0
19	7.372244531453313E-5	0.0	0.0	4.763796971334502	0.0
20	1.4744489062906626E-4	0.0	0.0	4.944638129691051	0.0
21	1.4744489062906626E-4	0.0	0.0	5.178780616010009	0.0
22	1.4744489062906626E-4	0.0	0.0	5.42095884886825	0.0
23	1.4744489062906626E-4	0.0	0.0	5.716585854579527	0.0
24	2.211673359435994E-4	0.0	0.0	5.924704317702455	0.0
25	2.211673359435994E-4	0.0	0.0	6.087557199402259	0.0
26	2.211673359435994E-4	0.0	0.0	6.261837060125814	0.0
27	2.211673359435994E-4	0.0	0.0	6.423657827591215	0.0
28	2.948897812581325E-4	0.0	0.0	6.5865107092910185	0.0
29	2.948897812581325E-4	0.0	0.0	6.766319753413165	0.0
30	2.948897812581325E-4	0.0	0.0	6.991173211622491	0.0
31	2.948897812581325E-4	0.0	0.0	7.203862466354919	0.0
32	2.948897812581325E-4	0.0	0.0	7.398637166875916	0.0
33	2.948897812581325E-4	0.0	0.0	7.586998014654547	0.0
34	2.948897812581325E-4	0.0	0.0	7.790766853503917	0.0
35	2.948897812581325E-4	0.0	0.0	8.037515877971659	0.0
36	2.948897812581325E-4	0.0	0.0	8.250794912266604	0.0
37	2.948897812581325E-4	0.0	0.0	8.465990730139726	0.0
38	2.948897812581325E-4	0.0	0.0	8.664230385590505	0.0
39	2.948897812581325E-4	0.0	0.0	8.883480937955927	0.0
40	3.686122265726656E-4	0.0	0.0	9.110103734852801	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAGT	35	1.01974365E-7	46.000004	46
GCGTTGC	35	1.01974365E-7	46.000004	26
TCGGCCA	35	1.01974365E-7	46.000004	10
AATGCGT	35	1.01974365E-7	46.000004	23
TCTACGG	35	1.01974365E-7	46.000004	2
GAACGAC	35	1.01974365E-7	46.000004	22
CGTCAAC	35	1.01974365E-7	46.000004	32
GTACGTC	35	1.01974365E-7	46.000004	22
CGAAGTA	70	0.0	46.000004	35
ACGGTCT	140	0.0	46.000004	30
CGGACAC	25	3.417484E-5	46.0	43
CTTCGTA	20	6.3118583E-4	46.0	13
TCGCCCT	20	6.3118583E-4	46.0	16
ACTATCG	30	1.8616538E-6	46.0	28
GTACCGG	25	3.417484E-5	46.0	2
GTACCCG	20	6.3118583E-4	46.0	21
CAAACGG	30	1.8616538E-6	46.0	2
CGAACGG	25	3.417484E-5	46.0	2
CGAACCC	40	5.6134013E-9	46.0	45
ACACGAT	20	6.3118583E-4	46.0	18
>>END_MODULE