FastQCFastQC Report
Thu 26 May 2016
SRR1544728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544728_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1308023
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47560.36360216907500864No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45980.3515228707752081No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT17100.1307316461560691Illumina Single End Adapter 2 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14840.11345366251205063No Hit
GGTTTAGGGATTCAAAATCTTTTTATTTTGTTCAGGAATATGGCTATATCTC14400.11008980728932137No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT14290.10924884348363904Illumina Single End Adapter 2 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAC453.110472E-1046.020
TCTGCGA206.311775E-446.029
CGGGTTG650.046.06
AGCGTAA206.311775E-446.010
AATCCGT206.311775E-446.031
TCGTTCG405.6115823E-946.02
GTACCGG650.046.02
GCCCAAT551.8189894E-1246.014
CGAACCG453.110472E-1046.012
CGAACAG405.6115823E-946.02
ACGCCTC253.4174158E-546.034
ACGCCGG301.861601E-646.027
CATATCG253.4174158E-546.01
GATCGTC206.311775E-446.09
CGTATCC253.4174158E-546.034
CGTATAA206.311775E-446.035
GATCGGT501.6370905E-1146.010
GATCGGC206.311775E-446.010
GTCGAAG850.046.01
GTACACG501.6370905E-1146.01
Produced by FastQC (version 0.11.5)