##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544728_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1308023
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76190403379757	33.0	31.0	34.0	31.0	34.0
2	33.167974875059535	34.0	33.0	34.0	31.0	34.0
3	33.18288057625898	34.0	33.0	34.0	31.0	34.0
4	36.48740809603501	37.0	37.0	37.0	35.0	37.0
5	36.48526058028032	37.0	37.0	37.0	35.0	37.0
6	36.557715728240254	37.0	37.0	37.0	35.0	37.0
7	36.67805917785849	37.0	37.0	37.0	35.0	37.0
8	36.68683731096471	37.0	37.0	37.0	35.0	37.0
9	38.45536508150086	39.0	39.0	39.0	37.0	39.0
10	38.319810125662926	39.0	39.0	39.0	37.0	39.0
11	38.07551319816242	39.0	39.0	39.0	35.0	39.0
12	37.20404992878566	39.0	35.0	39.0	35.0	39.0
13	37.10038967204705	39.0	35.0	39.0	35.0	39.0
14	38.18027970456177	40.0	35.0	41.0	35.0	41.0
15	38.230167971052495	40.0	35.0	41.0	35.0	41.0
16	38.30684705085461	40.0	35.0	41.0	35.0	41.0
17	38.28275878940967	40.0	35.0	41.0	35.0	41.0
18	38.354786574853804	40.0	36.0	41.0	35.0	41.0
19	38.31828951019975	40.0	36.0	41.0	35.0	41.0
20	38.17543269499084	40.0	35.0	41.0	35.0	41.0
21	38.093027416184576	40.0	35.0	41.0	35.0	41.0
22	38.01702263645211	40.0	35.0	41.0	35.0	41.0
23	37.96000070335155	40.0	35.0	41.0	35.0	41.0
24	37.92547608107809	40.0	35.0	41.0	35.0	41.0
25	37.91947389304317	40.0	35.0	41.0	35.0	41.0
26	37.87747386704974	40.0	35.0	41.0	35.0	41.0
27	37.86764605821151	40.0	35.0	41.0	35.0	41.0
28	37.867876176489254	40.0	35.0	41.0	35.0	41.0
29	37.85314172610115	40.0	35.0	41.0	35.0	41.0
30	37.8153702190252	40.0	35.0	41.0	35.0	41.0
31	37.68884262738499	40.0	35.0	41.0	35.0	41.0
32	37.57104653358542	40.0	35.0	41.0	35.0	41.0
33	37.45034452758094	40.0	35.0	41.0	35.0	41.0
34	37.35192271083918	40.0	35.0	41.0	35.0	41.0
35	37.23683757854411	39.0	35.0	41.0	34.0	41.0
36	37.170701891327596	39.0	35.0	41.0	34.0	41.0
37	37.14009463136352	39.0	35.0	41.0	34.0	41.0
38	37.03603682809859	39.0	35.0	41.0	33.0	41.0
39	37.07609575672599	39.0	35.0	41.0	34.0	41.0
40	36.94130072636337	39.0	35.0	41.0	33.0	41.0
41	36.95775915255313	39.0	35.0	41.0	34.0	41.0
42	36.94988620230684	39.0	35.0	41.0	34.0	41.0
43	36.891640284612734	39.0	35.0	41.0	34.0	41.0
44	36.800966802571516	39.0	35.0	41.0	34.0	41.0
45	36.723025512548325	38.0	35.0	41.0	34.0	41.0
46	36.69208874767492	38.0	35.0	41.0	34.0	41.0
47	36.64411176256075	38.0	35.0	41.0	33.0	41.0
48	36.6174830259101	38.0	35.0	41.0	34.0	41.0
49	36.62601575048757	38.0	35.0	41.0	34.0	41.0
50	36.487661914201816	38.0	35.0	41.0	33.0	41.0
51	36.4303915145223	38.0	35.0	41.0	33.0	41.0
52	36.18880019693843	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	3.0
15	10.0
16	23.0
17	71.0
18	176.0
19	275.0
20	525.0
21	831.0
22	1340.0
23	2249.0
24	3886.0
25	6532.0
26	8845.0
27	9901.0
28	9707.0
29	9708.0
30	10662.0
31	12936.0
32	16710.0
33	23808.0
34	66166.0
35	326278.0
36	45141.0
37	52914.0
38	109552.0
39	587744.0
40	2024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.177127619315563	24.808279365118196	30.6116941368768	13.402898878689443
2	30.120418371848203	25.705893550801477	30.189071598893904	13.984616478456418
3	27.561365511156914	26.280195378827436	32.2489742152852	13.909464894730444
4	24.73786775920607	28.589940696761452	32.20470893860429	14.467482605428192
5	22.81106677787776	34.90015083832624	28.5737330306883	13.715049353107705
6	21.000624606753856	43.567200270943246	24.3252603356363	11.106914786666595
7	90.74909233247428	3.543439220869969	4.4669703820192765	1.240498064636478
8	91.54005701734602	2.480690324252708	4.469875529711634	1.5093771286896331
9	87.60962154335206	4.798157218947985	5.713431644550592	1.878789593149356
10	51.80038883108325	31.50105158701338	10.025588235069261	6.672971346834116
11	44.244940647068134	19.48742491531112	24.61615736114732	11.651477076473427
12	42.33159508663074	20.73625616674936	24.50201563733971	12.430133109280188
13	20.32724195216751	42.46928379699745	24.613863823495457	12.589610427339581
14	13.339673690753145	46.27579178653586	27.575738347108576	12.808796175602415
15	11.974789434130745	25.468741757599062	51.02417923843847	11.532289569831724
16	14.569927287211309	21.880578552517807	48.948451212249324	14.601042948021556
17	14.764342828834048	20.019525650542842	30.19763413946085	35.018497381162256
18	18.411602854078254	23.85042159044604	39.37728923726876	18.36068631820694
19	27.074523918921916	24.110279406401876	29.00078974146479	19.81440693321142
20	30.025695266826347	23.808067595141676	29.295050622198538	16.871186515833436
21	19.749423366408696	30.3435031341192	32.094848485080156	17.81222501439195
22	20.737555838085417	23.564570347769116	27.857919929542525	27.83995388460295
23	14.711285657821001	31.9375118021625	27.634835167271522	25.716367372744976
24	14.904248625597564	24.208595720411644	45.1262707154232	15.760884938567592
25	13.511077404602212	26.36115725793812	40.731164513162234	19.396600824297433
26	13.907553613353892	37.07939386386936	29.408733638475777	19.604318884300966
27	13.773458111975096	40.687205041501564	30.349236978248857	15.190099868274487
28	13.52269799537164	33.17128215635352	38.739303513776136	14.566716334498706
29	14.033468830441054	25.86720569898236	38.29519817312081	21.80412729745578
30	16.6761593641702	37.33458815326642	30.499004986915367	15.490247495648013
31	27.57543254208833	30.639140137444066	26.616122193569993	15.169305126897617
32	28.400800291737987	25.69794262027503	31.654718609688054	14.246538478298929
33	28.41479087141434	29.042379224218536	26.058104482872242	16.48472542149488
34	19.091789670365124	29.773176771356468	27.222609999977067	23.912423558301345
35	17.879425667591473	27.15571515179779	33.27617327829862	21.688685902312116
36	31.94722111155538	24.908048252974144	27.645308989215025	15.499421646255456
37	17.60305438054224	36.877791904270794	30.177833264399784	15.34132045078718
38	18.22934306201038	35.94455143372861	25.460179217032113	20.365926287228895
39	19.37809962057242	34.87377515532984	29.322649525275928	16.42547569882181
40	25.391525989986413	25.162248676055395	27.275743622245173	22.17048171171302
41	15.979153271769686	26.1483934151005	29.864000862370155	28.00845245075966
42	20.058668693134603	24.342079611749945	28.05592868015318	27.543323014962272
43	20.390084883828496	25.189541774112534	28.734968727614117	25.685404614444852
44	16.460261019875034	30.30680653168943	32.11151485868368	21.12141758975186
45	15.50821354058759	42.44497229788773	24.908812765524765	17.138001395999915
46	21.65122478733172	35.72024345137662	26.558325044743096	16.07020671654856
47	17.942268599252458	30.711386573477682	27.417484249130176	23.928860578139684
48	19.50913707174874	28.048207103391913	34.485096974594484	17.957558850264867
49	22.468718057709992	24.779610144469938	35.13455038634642	17.61712141147365
50	20.226632100505878	36.49721755657202	26.541735122394638	16.734415220527467
51	17.47087016053999	35.222010622137375	25.80023439954802	21.506884817774612
52	18.769776984043858	28.92357397385214	35.329730440519775	16.976918601584224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5270.0
1	4467.5
2	3665.0
3	6054.0
4	8443.0
5	5890.0
6	3337.0
7	3472.0
8	3607.0
9	3842.0
10	4077.0
11	4275.5
12	4474.0
13	4314.0
14	4285.5
15	4417.0
16	3894.0
17	3371.0
18	3687.5
19	4004.0
20	4851.0
21	5698.0
22	5979.0
23	6260.0
24	7871.5
25	9483.0
26	12362.0
27	15241.0
28	18038.5
29	20836.0
30	24254.0
31	27672.0
32	32036.5
33	36401.0
34	44034.5
35	51668.0
36	55188.0
37	58708.0
38	64045.0
39	83725.0
40	98068.0
41	118980.0
42	139892.0
43	156839.0
44	173786.0
45	160259.0
46	146732.0
47	131521.5
48	116311.0
49	103272.5
50	90234.0
51	79709.0
52	69184.0
53	56687.5
54	44191.0
55	35406.0
56	26621.0
57	22595.0
58	18569.0
59	15901.0
60	13233.0
61	10923.5
62	8614.0
63	6784.0
64	4180.0
65	3406.0
66	2578.0
67	1750.0
68	1411.5
69	1073.0
70	833.0
71	593.0
72	487.0
73	381.0
74	259.0
75	137.0
76	95.0
77	53.0
78	37.0
79	21.0
80	16.0
81	11.0
82	17.5
83	24.0
84	12.5
85	1.0
86	7.5
87	14.0
88	8.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1308023.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.5210175391728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67598161702122	10.232162747378624
2	10.232278400610411	2.7670163144070474
3	3.650211119502554	1.4806370570543288
4	1.7266264380663903	0.9338298541078048
5	0.9775582570940293	0.6608791169865781
6	0.6378595233934674	0.5174705881998886
7	0.459704780625156	0.43509734811720235
8	0.35671267114706984	0.38584946264197684
9	0.28220367556311055	0.3434112762217063
>10	2.9120842363329036	9.36398609421679
>50	1.115803041572241	10.85465130560246
>100	1.941713290886793	58.55666053282091
>500	0.027852444746326945	2.283462515688535
>1k	0.0034105034383257486	1.1848857865561626
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4756	0.36360216907500864	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4598	0.3515228707752081	No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCTT	1710	0.1307316461560691	Illumina Single End Adapter 2 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1484	0.11345366251205063	No Hit
GGTTTAGGGATTCAAAATCTTTTTATTTTGTTCAGGAATATGGCTATATCTC	1440	0.11008980728932137	No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	1429	0.10924884348363904	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14273449320080764	0.0
2	0.0	0.0	0.0	0.4210935128816542	0.0
3	0.0	0.0	0.0	0.5702499115076722	0.0
4	0.0	0.0	0.0	0.739895246490314	0.0
5	0.0	0.0	0.0	1.205789194838317	0.0
6	0.0	0.0	0.0	1.511211958811122	0.0
7	0.0	0.0	0.0	1.708685550636342	0.0
8	0.0	0.0	0.0	2.136277420198269	0.0
9	0.0	0.0	0.0	2.283751891212922	0.0
10	0.0	0.0	0.0	2.5855814461978115	0.0
11	0.0	0.0	0.0	3.0041520676624187	0.0
12	0.0	0.0	0.0	3.329528609206413	0.0
13	0.0	0.0	0.0	3.4570493026498768	0.0
14	0.0	0.0	0.0	3.500244261759923	0.0
15	0.0	0.0	0.0	3.5770013218422	0.0
16	0.0	0.0	0.0	3.760713687756255	0.0
17	0.0	0.0	0.0	3.991749380553706	0.0
18	0.0	0.0	0.0	4.318960752219189	0.0
19	0.0	0.0	0.0	4.469646175946448	0.0
20	0.0	0.0	0.0	4.6253773825078	0.0
21	0.0	0.0	0.0	4.8417344343333415	0.0
22	0.0	0.0	0.0	5.052281190774169	0.0
23	0.0	0.0	0.0	5.299830354665017	0.0
24	0.0	0.0	0.0	5.470393104708403	0.0
25	0.0	0.0	0.0	5.616338550621816	0.0
26	0.0	0.0	0.0	5.763889472891532	0.0
27	0.0	0.0	0.0	5.9028778545943	0.0
28	7.645125506202873E-5	0.0	0.0	6.052875217026	0.0
29	7.645125506202873E-5	0.0	0.0	6.211588022534772	0.0
30	7.645125506202873E-5	0.0	0.0	6.41953543630349	0.0
31	7.645125506202873E-5	0.0	0.0	6.621825457197618	0.0
32	7.645125506202873E-5	0.0	0.0	6.791394340925198	0.0
33	7.645125506202873E-5	0.0	0.0	6.950565853964342	0.0
34	7.645125506202873E-5	0.0	0.0	7.123727946679836	0.0
35	7.645125506202873E-5	0.0	0.0	7.362026508708181	0.0
36	7.645125506202873E-5	0.0	0.0	7.549714339885461	0.0
37	7.645125506202873E-5	0.0	0.0	7.739389903694354	0.0
38	1.5290251012405745E-4	0.0	0.0	7.911252325073795	0.0
39	1.5290251012405745E-4	0.0	0.0	8.100163376332068	0.0
40	1.5290251012405745E-4	0.0	0.0	8.294578917954807	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAC	45	3.110472E-10	46.0	20
TCTGCGA	20	6.311775E-4	46.0	29
CGGGTTG	65	0.0	46.0	6
AGCGTAA	20	6.311775E-4	46.0	10
AATCCGT	20	6.311775E-4	46.0	31
TCGTTCG	40	5.6115823E-9	46.0	2
GTACCGG	65	0.0	46.0	2
GCCCAAT	55	1.8189894E-12	46.0	14
CGAACCG	45	3.110472E-10	46.0	12
CGAACAG	40	5.6115823E-9	46.0	2
ACGCCTC	25	3.4174158E-5	46.0	34
ACGCCGG	30	1.861601E-6	46.0	27
CATATCG	25	3.4174158E-5	46.0	1
GATCGTC	20	6.311775E-4	46.0	9
CGTATCC	25	3.4174158E-5	46.0	34
CGTATAA	20	6.311775E-4	46.0	35
GATCGGT	50	1.6370905E-11	46.0	10
GATCGGC	20	6.311775E-4	46.0	10
GTCGAAG	85	0.0	46.0	1
GTACACG	50	1.6370905E-11	46.0	1
>>END_MODULE