FastQCFastQC Report
Thu 26 May 2016
SRR1544727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544727_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1169383
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83730.7160186183654115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56240.4809373832183297No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT24380.20848601356441818Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT22330.19095540126716398No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGT20920.1788977606139306No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18290.15640726776428251No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT15380.13152234982037536No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT14470.12374046826403326No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT206.311499E-446.00000441
TCGTTTG351.0195981E-746.0000041
AGGTAAC206.311499E-446.00000412
CGAACCA351.0195981E-746.00000422
CGTATCA206.311499E-446.00000429
CAAGTCG206.311499E-446.0000041
GATCGAT206.311499E-446.0000049
CGTGAGA206.311499E-446.00000414
CGGTTGA206.311499E-446.00000435
ACTCTCG351.0195981E-746.00000423
CGACCGT206.311499E-446.00000424
AGGCGTG206.311499E-446.0000041
CACGAAA206.311499E-446.00000435
TACGGAT351.0195981E-746.00000426
TACGGAC206.311499E-446.00000420
ACGGGTC351.0195981E-746.0000045
TCGGCGA551.8189894E-1246.00000412
AACGGTT206.311499E-446.00000436
ATCCGAT700.046.00000419
TGCGCGA206.311499E-446.00000420
Produced by FastQC (version 0.11.5)