FastQCFastQC Report
Thu 26 May 2016
SRR1544726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544726_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1619728
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57120.3526518032657335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48300.29819821599676No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT20750.1281079292325625Illumina Single End Adapter 1 (95% over 21bp)
AAGGAGGGGTTTTATAACCTGAAATTTTTTCCCTTTTTCCTTCTGTTTAAAA20490.1265027214445882No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT17070.10538806515661889No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGT351.01992555E-746.00000438
CGTCTAC351.01992555E-746.00000442
CGCGATT351.01992555E-746.00000415
ATATCGT351.01992555E-746.00000411
AACGCGA351.01992555E-746.00000413
CTCGAAC351.01992555E-746.00000420
ATGCGAT351.01992555E-746.00000434
TAAGGCG351.01992555E-746.0000041
CCGGTGT351.01992555E-746.00000434
CCGAACG351.01992555E-746.00000413
TATCGTA351.01992555E-746.00000412
CGTCAAA351.01992555E-746.00000418
GCGTAAG700.046.0000041
CTTCGCC206.312224E-446.021
AACGTCG253.4177792E-546.01
AACGTAT301.8618775E-646.010
AGACTCG206.312224E-446.06
AACCGGT405.6134013E-946.027
GTCGCTC301.8618775E-646.013
TCGTTCC253.4177792E-546.032
Produced by FastQC (version 0.11.5)