FastQCFastQC Report
Thu 26 May 2016
SRR1544725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544725_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1436649
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67680.4710962803022868No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66050.4597504331259758No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT23870.1661505350297811RNA PCR Primer, Index 41 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT23400.16287903308323745RNA PCR Primer, Index 41 (96% over 27bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20440.14227553146245187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAC351.0198164E-746.00000428
CTATCGA351.0198164E-746.00000440
GACGTAG351.0198164E-746.0000041
ATCGTAG700.046.0000041
GACGATA351.0198164E-746.00000435
TGCCGAT351.0198164E-746.00000445
TGATCGC206.3119846E-446.046
CTTCGTA453.110472E-1046.024
TCGCCAA206.3119846E-446.045
CTTAACG253.4175853E-546.01
CTATGCG405.6134013E-946.01
CGTATGA600.046.015
TCCGCAT301.8617302E-646.017
CTATCGT253.4175853E-546.015
ACGTTAG453.110472E-1046.01
TCCGAAC206.3119846E-446.026
CGCATCG206.3119846E-446.021
TAGAACG1000.046.01
GAATCGG650.046.02
CGACCGT206.3119846E-446.022
Produced by FastQC (version 0.11.5)