##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544725_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1436649
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69425586903969	33.0	31.0	34.0	31.0	34.0
2	33.09765502916857	34.0	33.0	34.0	31.0	34.0
3	33.13060323015573	34.0	33.0	34.0	31.0	34.0
4	36.45291229799346	37.0	37.0	37.0	35.0	37.0
5	36.45025750896705	37.0	37.0	37.0	35.0	37.0
6	36.51919919200863	37.0	37.0	37.0	35.0	37.0
7	36.62772048008943	37.0	37.0	37.0	35.0	37.0
8	36.63384723756464	37.0	37.0	37.0	35.0	37.0
9	38.37307999379111	39.0	39.0	39.0	37.0	39.0
10	38.2568163831249	39.0	39.0	39.0	37.0	39.0
11	38.10157387086198	39.0	39.0	39.0	35.0	39.0
12	37.34749754463338	39.0	35.0	39.0	35.0	39.0
13	37.226451972611265	39.0	35.0	39.0	35.0	39.0
14	38.38195342077292	40.0	35.0	41.0	35.0	41.0
15	38.392319209493756	40.0	35.0	41.0	35.0	41.0
16	38.478597764659284	40.0	35.0	41.0	35.0	41.0
17	38.41591926768473	40.0	35.0	41.0	35.0	41.0
18	38.478702870360124	40.0	36.0	41.0	35.0	41.0
19	38.4430309699864	40.0	36.0	41.0	35.0	41.0
20	38.28182457928137	40.0	35.0	41.0	35.0	41.0
21	38.210626952025166	40.0	35.0	41.0	35.0	41.0
22	38.17022111872837	40.0	35.0	41.0	35.0	41.0
23	38.09315218957449	40.0	35.0	41.0	35.0	41.0
24	38.061190311621004	40.0	35.0	41.0	35.0	41.0
25	38.076631800808684	40.0	35.0	41.0	35.0	41.0
26	38.02104480635145	40.0	35.0	41.0	35.0	41.0
27	37.99715588149924	40.0	35.0	41.0	35.0	41.0
28	37.99061775005586	40.0	35.0	41.0	35.0	41.0
29	37.94694807151921	40.0	35.0	41.0	35.0	41.0
30	37.89902752864479	40.0	35.0	41.0	35.0	41.0
31	37.7687639778401	40.0	35.0	41.0	35.0	41.0
32	37.63373169090014	40.0	35.0	41.0	35.0	41.0
33	37.49129606466158	40.0	35.0	41.0	34.0	41.0
34	37.36825000400237	40.0	35.0	41.0	34.0	41.0
35	37.236409171620906	40.0	35.0	41.0	34.0	41.0
36	37.16135604451748	40.0	35.0	41.0	34.0	41.0
37	37.123021698410675	40.0	35.0	41.0	34.0	41.0
38	37.01906589570591	40.0	35.0	41.0	33.0	41.0
39	37.030145846341036	39.0	35.0	41.0	33.0	41.0
40	36.95139104958831	39.0	35.0	41.0	33.0	41.0
41	36.93502588314891	39.0	35.0	41.0	33.0	41.0
42	36.91030168120397	39.0	35.0	41.0	33.0	41.0
43	36.859757672194114	39.0	35.0	41.0	33.0	41.0
44	36.760523969320275	39.0	35.0	41.0	33.0	41.0
45	36.68509079113966	39.0	35.0	41.0	33.0	41.0
46	36.6365994755852	39.0	35.0	41.0	33.0	41.0
47	36.57241887197221	38.0	35.0	41.0	33.0	41.0
48	36.52551667108668	38.0	35.0	41.0	33.0	41.0
49	36.53630984325329	38.0	35.0	41.0	33.0	41.0
50	36.40195134650148	38.0	35.0	41.0	33.0	41.0
51	36.33991670895257	38.0	35.0	41.0	33.0	41.0
52	36.12867374007151	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	9.0
15	16.0
16	32.0
17	84.0
18	166.0
19	328.0
20	592.0
21	1081.0
22	1682.0
23	2960.0
24	4864.0
25	8085.0
26	10871.0
27	11887.0
28	11847.0
29	12063.0
30	13152.0
31	15542.0
32	19455.0
33	27288.0
34	66907.0
35	308539.0
36	59004.0
37	69954.0
38	136339.0
39	651239.0
40	2659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.158132571003772	24.87670962079116	29.839926105819863	14.125231702385202
2	30.105265795611874	26.67492198859986	29.350871368023785	13.868940847764485
3	28.379304896324708	26.119393115506988	32.30636014781621	13.194941840352097
4	25.023579176263656	29.064997783035384	30.950914245581213	14.960508795119754
5	22.109645431834775	34.118772226201386	29.765029593171334	14.006552748792503
6	20.56396517172949	43.30911725828647	25.74456252014236	10.382355049841681
7	88.31036669360435	4.090839168091858	5.790697658231064	1.8080964800727246
8	88.99411060043198	3.0040740640198127	5.949330699426234	2.0524846361219753
9	84.57744375974924	5.270180816608649	7.410160728194569	2.742214695447531
10	42.734028979938735	33.4409448654473	12.582892550650854	11.242133603963111
11	41.76510755236665	20.470135711645643	23.84897076460569	13.915785971382014
12	39.52113564273528	20.507444755121117	25.602078169406724	14.369341432736876
13	20.842599688580858	39.96954022868495	25.696603693734517	13.491256388999679
14	15.06088125909669	41.77227701407929	28.0999743152294	15.06686741159462
15	13.087678340360101	25.188198369956755	48.20230968037426	13.521813609308886
16	16.90183197148364	20.477165960509492	45.455013715945924	17.165988352060943
17	15.474413026424688	20.235422848587234	31.079129279315964	33.21103484567212
18	18.823665349017052	23.133834360376127	39.562064220279275	18.480436070327546
19	27.65150012285534	23.795025785699917	28.367123772055663	20.186350319389078
20	28.87170074249173	23.525300891171053	29.407252571783367	18.195745794553854
21	19.412744518668095	29.42249637872577	32.98704137197046	18.177717730635667
22	20.973111734320632	23.154855500543277	27.593309152061497	28.27872361307459
23	15.351488080943918	32.067958144264885	27.903057740617225	24.67749603417397
24	15.026495685445784	24.87684883363995	42.46841086444915	17.62824461646512
25	15.004778481034686	25.948926982164743	39.94176726535152	19.104527271449044
26	14.459551358752206	35.510691894819125	29.138989412166787	20.890767334261884
27	14.542661429479296	38.38411470025038	29.13460420742993	17.938619662840402
28	13.680446650504056	30.36308799156927	38.060932071786496	17.89553328614018
29	13.778034857505208	25.005899144467435	38.29501847702536	22.921047521002
30	16.99587025084067	34.01220479045334	30.847687918204098	18.14423704050189
31	26.515940915282716	29.906121815419073	26.693228478215623	16.88470879108258
32	27.99514704009121	25.79412229431128	29.082817027680385	17.127913637917125
33	26.785665809811583	28.193455743191276	24.94179162760006	20.079086819397084
34	17.691516856239765	28.779541836593346	27.31453542236134	26.21440588480554
35	16.92925690269509	26.804807576520084	34.30023617459797	21.965699346186856
36	28.920216420294725	25.555720290759954	27.206645464549794	18.31741782439552
37	18.33029501290851	34.22025839296864	30.16707630047423	17.282370293648622
38	17.984629509365195	33.953317755415554	27.119567827632217	20.94248490758703
39	19.046893152050362	33.2163249339261	28.1038026685711	19.63297924545244
40	23.42945284477976	26.043174080794962	27.773659397667767	22.75371367675751
41	16.037180967654592	25.628946249223016	29.80790715059837	28.52596563252402
42	18.940256109877918	23.660406960920866	29.392217584114142	28.007119345087073
43	18.858329348365537	24.858820769721763	30.023269427675096	26.259580454237604
44	16.28567590274312	28.4432732003433	32.63852200502698	22.632528891886604
45	15.936182045858105	39.91420312129128	25.31279386962299	18.83682096322762
46	20.514475003984966	34.18343659446392	26.633784591782682	18.668303809768428
47	18.53319774001861	28.5453162185057	28.0654495287297	24.85603651274598
48	18.648048340269614	26.76401821182488	34.53557549547593	20.052357952429578
49	21.176919344947862	24.642344789854725	34.924884227114624	19.255851638082788
50	19.318567026462276	34.255618456561066	28.09802533534635	18.32778918163031
51	16.67978747766504	33.55189750593221	27.451451259145415	22.31686375725734
52	17.74706278290661	28.08626184962367	35.2315005265726	18.935174840897115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7570.0
1	6521.5
2	5473.0
3	8950.0
4	12427.0
5	8333.0
6	4239.0
7	4419.5
8	4600.0
9	4985.5
10	5371.0
11	5553.5
12	5736.0
13	5520.0
14	5854.5
15	6405.0
16	5845.0
17	5285.0
18	4883.5
19	4482.0
20	5561.0
21	6640.0
22	6455.5
23	6271.0
24	8683.5
25	11096.0
26	13547.0
27	15998.0
28	16770.5
29	17543.0
30	23462.5
31	29382.0
32	33872.5
33	38363.0
34	42611.5
35	46860.0
36	50113.5
37	53367.0
38	59449.5
39	83176.5
40	100821.0
41	122602.5
42	144384.0
43	158594.0
44	172804.0
45	160018.0
46	147232.0
47	137221.5
48	127211.0
49	117658.0
50	108105.0
51	92368.0
52	76631.0
53	66971.0
54	57311.0
55	49798.0
56	42285.0
57	36585.0
58	30885.0
59	25957.5
60	21030.0
61	18602.5
62	16175.0
63	13693.5
64	9711.5
65	8211.0
66	6733.5
67	5256.0
68	4226.0
69	3196.0
70	2764.5
71	2333.0
72	1964.0
73	1595.0
74	1168.5
75	742.0
76	688.0
77	634.0
78	488.0
79	342.0
80	254.5
81	167.0
82	125.5
83	84.0
84	53.0
85	22.0
86	22.0
87	22.0
88	16.0
89	7.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1436649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.33604838861182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.08065098179037	12.101850990983912
2	10.51980013125239	3.4370392796532805
3	3.93373633324983	1.9278512126402896
4	1.9603963998783418	1.28100521797092
5	1.1477434678214413	0.9374796414022101
6	0.7927232846211906	0.7769979561810642
7	0.5727166810479265	0.6549149190194862
8	0.44097312040961256	0.5763006586470861
9	0.3512745683977124	0.5164594512330385
>10	3.765646290558046	14.214883424507946
>50	0.9840123883369751	11.423303512362878
>100	1.433198199667336	49.25773690201825
>500	0.014987133847731328	1.4850721267619669
>1k	0.0012846114726626852	0.4736421747670879
>5k	8.564076484417901E-4	0.9354625318505786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6768	0.4710962803022868	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6605	0.4597504331259758	No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT	2387	0.1661505350297811	RNA PCR Primer, Index 41 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	2340	0.16287903308323745	RNA PCR Primer, Index 41 (96% over 27bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2044	0.14227553146245187	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17283275177165752	0.0
2	0.0	0.0	0.0	0.4946232517476433	0.0
3	0.0	0.0	0.0	0.6903565171451064	0.0
4	0.0	0.0	0.0	0.9232596131692571	0.0
5	0.0	0.0	0.0	1.5527105089691358	0.0
6	0.0	0.0	0.0	2.014549134826948	0.0
7	0.0	0.0	0.0	2.3031373703667355	0.0
8	0.0	0.0	0.0	2.9400361535768305	0.0
9	0.0	0.0	0.0	3.1560248884731066	0.0
10	0.0	0.0	0.0	3.6161929601454497	0.0
11	0.0	0.0	0.0	4.274112883522697	0.0
12	0.0	0.0	0.0	4.765255814050613	0.0
13	0.0	0.0	0.0	4.9524274892475475	0.0
14	0.0	0.0	0.0	5.00713813882166	0.0
15	0.0	0.0	0.0	5.129018987936511	0.0
16	0.0	0.0	0.0	5.401806565138736	0.0
17	0.0	0.0	0.0	5.7538062533019545	0.0
18	0.0	0.0	0.0	6.221770244506487	0.0
19	1.3921284878909185E-4	0.0	0.0	6.425229823011745	0.0
20	2.0881927318363775E-4	0.0	0.0	6.670940501124492	0.0
21	2.0881927318363775E-4	0.0	0.0	6.967115836923285	0.0
22	2.0881927318363775E-4	0.0	0.0	7.25674816882899	0.0
23	2.0881927318363775E-4	0.0	0.0	7.643272643491904	0.0
24	3.4803212197272957E-4	0.0	0.0	7.867962181437498	0.0
25	3.4803212197272957E-4	0.0	0.0	8.064809149625274	0.0
26	3.4803212197272957E-4	0.0	0.0	8.265136439032776	0.0
27	3.4803212197272957E-4	0.0	0.0	8.462957897162077	0.0
28	3.4803212197272957E-4	0.0	0.0	8.709503852367558	6.960642439454592E-5
29	3.4803212197272957E-4	0.0	0.0	8.940457968508662	6.960642439454592E-5
30	3.4803212197272957E-4	0.0	0.0	9.207259393212956	6.960642439454592E-5
31	4.176385463672755E-4	0.0	0.0	9.463967886380042	6.960642439454592E-5
32	4.176385463672755E-4	0.0	0.0	9.69408672542841	6.960642439454592E-5
33	4.176385463672755E-4	0.0	0.0	9.982466141695014	6.960642439454592E-5
34	4.176385463672755E-4	0.0	0.0	10.223791615070905	6.960642439454592E-5
35	4.176385463672755E-4	0.0	0.0	10.531660830167981	6.960642439454592E-5
36	4.176385463672755E-4	0.0	0.0	10.773195122817055	6.960642439454592E-5
37	4.176385463672755E-4	0.0	0.0	11.012780435583082	6.960642439454592E-5
38	4.176385463672755E-4	0.0	0.0	11.257307804481123	6.960642439454592E-5
39	4.176385463672755E-4	0.0	0.0	11.513181020555473	6.960642439454592E-5
40	4.176385463672755E-4	0.0	0.0	11.794669400807017	6.960642439454592E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAC	35	1.0198164E-7	46.000004	28
CTATCGA	35	1.0198164E-7	46.000004	40
GACGTAG	35	1.0198164E-7	46.000004	1
ATCGTAG	70	0.0	46.000004	1
GACGATA	35	1.0198164E-7	46.000004	35
TGCCGAT	35	1.0198164E-7	46.000004	45
TGATCGC	20	6.3119846E-4	46.0	46
CTTCGTA	45	3.110472E-10	46.0	24
TCGCCAA	20	6.3119846E-4	46.0	45
CTTAACG	25	3.4175853E-5	46.0	1
CTATGCG	40	5.6134013E-9	46.0	1
CGTATGA	60	0.0	46.0	15
TCCGCAT	30	1.8617302E-6	46.0	17
CTATCGT	25	3.4175853E-5	46.0	15
ACGTTAG	45	3.110472E-10	46.0	1
TCCGAAC	20	6.3119846E-4	46.0	26
CGCATCG	20	6.3119846E-4	46.0	21
TAGAACG	100	0.0	46.0	1
GAATCGG	65	0.0	46.0	2
CGACCGT	20	6.3119846E-4	46.0	22
>>END_MODULE