FastQCFastQC Report
Thu 26 May 2016
SRR1544724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544724_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1174083
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47480.40440071102298564No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41110.35014560299399616No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT17030.1450493704448493TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT14870.1266520339703411TruSeq Adapter, Index 23 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14460.1231599469543465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG750.046.0000041
TAGCTAT750.046.00000411
TCACGAT405.6115823E-946.012
TCACGAA301.8614373E-646.022
CCTTCGC206.31151E-446.042
TCTGCGA301.8614373E-646.019
ACAACGT453.092282E-1046.030
AGCGTTA301.8614373E-646.030
CGTGGTA206.31151E-446.037
CTTCGAC301.8614373E-646.023
TATCACG301.8614373E-646.01
CGGAATT206.31151E-446.014
AGTCCCG206.31151E-446.024
TACCCGC206.31151E-446.029
TCGCAAT206.31151E-446.01
AAATCGA206.31151E-446.030
TCGCAAG551.8189894E-1246.01
GCGACGC206.31151E-446.032
TCCGCGA206.31151E-446.030
CTCGTCT206.31151E-446.019
Produced by FastQC (version 0.11.5)