##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544724_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1174083
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.701594350654936	33.0	31.0	34.0	31.0	34.0
2	33.09875792426941	34.0	33.0	34.0	31.0	34.0
3	33.13924058179873	34.0	33.0	34.0	31.0	34.0
4	36.4564915768306	37.0	37.0	37.0	35.0	37.0
5	36.452002115693695	37.0	37.0	37.0	35.0	37.0
6	36.51288452349621	37.0	37.0	37.0	35.0	37.0
7	36.63542867071578	37.0	37.0	37.0	35.0	37.0
8	36.641332852958435	37.0	37.0	37.0	35.0	37.0
9	38.395162863272866	39.0	39.0	39.0	37.0	39.0
10	38.26769572508928	39.0	39.0	39.0	37.0	39.0
11	37.99281822494662	39.0	39.0	39.0	35.0	39.0
12	36.893950427695486	39.0	35.0	39.0	35.0	39.0
13	36.753703102761904	39.0	35.0	39.0	35.0	39.0
14	37.73986081052191	40.0	35.0	41.0	35.0	41.0
15	37.79269353188829	40.0	35.0	41.0	35.0	41.0
16	37.90229736739225	40.0	35.0	41.0	35.0	41.0
17	37.889366424690586	39.0	35.0	41.0	35.0	41.0
18	37.98965234996163	39.0	36.0	41.0	35.0	41.0
19	37.92762777418632	39.0	36.0	41.0	35.0	41.0
20	37.74597792489969	39.0	35.0	41.0	35.0	41.0
21	37.64202530826185	39.0	35.0	41.0	35.0	41.0
22	37.590856012735046	39.0	35.0	41.0	35.0	41.0
23	37.553282008171486	39.0	35.0	41.0	35.0	41.0
24	37.505011996596494	38.0	35.0	41.0	35.0	41.0
25	37.48551933721892	38.0	35.0	41.0	35.0	41.0
26	37.40096909673336	38.0	35.0	41.0	35.0	41.0
27	37.386959865699446	38.0	35.0	41.0	35.0	41.0
28	37.424913741192064	38.0	35.0	41.0	35.0	41.0
29	37.421239384268404	38.0	35.0	41.0	35.0	41.0
30	37.39466800899085	38.0	35.0	41.0	35.0	41.0
31	37.24093782126136	38.0	35.0	41.0	35.0	41.0
32	37.0973125409362	38.0	35.0	41.0	34.0	41.0
33	36.95087911161306	38.0	35.0	41.0	34.0	41.0
34	36.826438164933826	38.0	35.0	41.0	34.0	41.0
35	36.708811046578475	38.0	35.0	41.0	34.0	41.0
36	36.61705603436895	38.0	35.0	41.0	33.0	41.0
37	36.571546474993674	37.0	35.0	41.0	33.0	41.0
38	36.45373879018775	37.0	35.0	41.0	33.0	41.0
39	36.48077265406279	37.0	35.0	41.0	33.0	41.0
40	36.34236335931957	37.0	35.0	41.0	32.0	41.0
41	36.37601089531149	37.0	35.0	41.0	33.0	41.0
42	36.37142774403513	37.0	35.0	41.0	33.0	41.0
43	36.31830373150791	36.0	35.0	41.0	33.0	41.0
44	36.21562359730956	36.0	35.0	41.0	33.0	41.0
45	36.122848214308526	36.0	35.0	41.0	33.0	41.0
46	36.11122211973089	36.0	35.0	40.0	33.0	41.0
47	36.043151974775206	36.0	35.0	40.0	33.0	41.0
48	36.01162694630618	36.0	35.0	40.0	33.0	41.0
49	36.02880545923925	36.0	35.0	40.0	33.0	41.0
50	35.849749975086944	36.0	35.0	40.0	33.0	41.0
51	35.75547043948341	35.0	35.0	40.0	32.0	41.0
52	35.50653914586959	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	8.0
14	19.0
15	18.0
16	41.0
17	90.0
18	152.0
19	318.0
20	575.0
21	912.0
22	1503.0
23	2516.0
24	4227.0
25	6813.0
26	9062.0
27	10083.0
28	10057.0
29	10545.0
30	11681.0
31	13543.0
32	17237.0
33	24449.0
34	70592.0
35	344450.0
36	44910.0
37	50343.0
38	98534.0
39	439515.0
40	1889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.14883445207877	23.99021193561273	30.184322573446682	13.676631038861817
2	28.902130428598323	25.967925606622362	31.53567507578255	13.594268888996774
3	28.32516951527277	25.709425994584713	32.074393377640256	13.89101111250227
4	24.256632623076904	28.186678454589664	32.71165667163224	14.845032250701184
5	23.114975687408812	34.095289685652546	28.832459033986524	13.957275592952117
6	21.214513795021304	43.46166327252843	24.74160685402991	10.58221607842035
7	89.40270832641303	3.899128085493104	5.063441000338136	1.6347225877557208
8	89.912127166478	2.9408483045917535	5.143077618873623	2.003946910056614
9	86.00950699396891	4.579488843633713	6.764342895689657	2.64666126670772
10	55.52188388725499	24.78513018244877	10.615859355769567	9.077126574526673
11	52.03575897104379	17.419892801445894	19.61794864587938	10.92639958163094
12	47.740832632786606	20.082481391860714	20.812497923911682	11.364188051440998
13	19.147198281552498	47.25202562340141	22.03711321942316	11.563662875622933
14	12.925236120444636	48.57774109666864	26.285364833661674	12.211657949225055
15	11.398427538768553	22.68349000879836	54.44155140650192	11.476531045931166
16	14.02583974046128	18.284908307163974	51.69898550613543	15.990266446239321
17	13.078121393461961	17.74210170831193	29.598248164737928	39.58152873348818
18	18.69791147644587	21.741733761582445	41.1532234092479	18.407131352723784
19	30.923963638005148	21.309566700139598	27.648471189856256	20.117998471998998
20	31.77986564834002	22.295186967190563	27.680922047248792	18.244025337220624
21	19.089962123631803	29.633680072022166	32.39549503740366	18.88086276694237
22	21.689182110634427	23.13848339512624	24.54306893124251	30.62926556299682
23	14.798783390952769	32.426838647693565	25.226751430691014	27.547626530662654
24	15.175502924409944	21.969826664724724	46.648490779612686	16.206179631252645
25	12.907179475386323	25.325211249971254	42.57680249181702	19.190806782825405
26	13.937345145104732	36.918684624511215	28.8373138866673	20.306656343716757
27	13.492657674116737	42.920049093633075	27.560061767353755	16.027231464896435
28	11.94336345897181	31.594018480805868	41.118983921920346	15.343634138301976
29	12.839637402125744	24.06712302281866	38.62946657093238	24.46377300412322
30	16.37098910383678	36.60286368169882	30.019768619424692	17.006378595039703
31	30.413522723691592	28.39458539132242	25.57212735385829	15.619764531127696
32	32.65365395802512	23.11437947743047	29.104671475526008	15.127295089018409
33	30.882995495207748	27.515942228956558	24.144034110024588	17.457028165811106
34	19.092602482107313	27.912421864553018	25.88786312381663	27.107112529523043
35	18.584205716290928	24.391205732473768	34.731105041125716	22.293483510109592
36	35.434547642713504	23.95929418959307	24.32485607916987	16.281302088523557
37	18.583694679166634	37.80064952818498	28.889695191907215	14.725960600741175
38	17.811432411507532	36.699790389606186	24.16106868083432	21.32770851805196
39	19.848511561789074	34.88152030137563	27.89836834363499	17.37159979320031
40	26.328802989226485	24.492902120207855	25.552793115989243	23.625501774576414
41	15.410409655876117	23.027929030571094	29.909554946285738	31.652106367267052
42	19.326146447908705	22.474049960692728	27.268770606507374	30.93103298489119
43	19.498365958795077	23.206792024073255	28.713302211172465	28.581539805959206
44	15.625556285203004	29.20355715907649	32.37530907099413	22.79557748472638
45	14.442249823905124	43.94859647912456	22.952125190467797	18.65702850650252
46	21.055070212242235	35.878213039452916	25.32495573140911	17.741761016895737
47	19.185696411582487	28.290504163674967	26.49437901749706	26.029420407245485
48	20.00684789746551	25.03775286755706	35.877190965204335	19.078208269773093
49	22.790211594921313	23.355674172950295	36.069170578229986	17.784943653898406
50	19.407656869233268	36.487624810171	26.7409544299679	17.363763890627833
51	16.288712126825786	35.6742240540064	25.255710201067554	22.781353618100255
52	18.034585289114993	27.853141558135157	36.611977177082025	17.500295975667818
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5401.0
1	4557.0
2	3713.0
3	5835.0
4	7957.0
5	5512.5
6	3068.0
7	3252.5
8	3437.0
9	3609.0
10	3781.0
11	3839.5
12	3898.0
13	3863.0
14	3772.0
15	3716.0
16	3489.5
17	3263.0
18	3370.0
19	3477.0
20	3615.5
21	3754.0
22	4564.5
23	5375.0
24	6086.0
25	6797.0
26	7831.0
27	8865.0
28	10918.5
29	12972.0
30	15479.0
31	17986.0
32	20852.0
33	23718.0
34	26314.0
35	28910.0
36	31602.5
37	34295.0
38	40170.0
39	68075.5
40	90106.0
41	110069.5
42	130033.0
43	140245.0
44	150457.0
45	145611.0
46	140765.0
47	127801.5
48	114838.0
49	102528.5
50	90219.0
51	78010.5
52	65802.0
53	56406.0
54	47010.0
55	39614.0
56	32218.0
57	27487.5
58	22757.0
59	20101.5
60	17446.0
61	15352.5
62	13259.0
63	11340.0
64	7668.5
65	5916.0
66	4734.5
67	3553.0
68	2828.0
69	2103.0
70	1790.5
71	1478.0
72	1299.0
73	1120.0
74	872.5
75	625.0
76	454.5
77	284.0
78	262.0
79	240.0
80	185.0
81	130.0
82	77.5
83	25.0
84	19.0
85	13.0
86	9.5
87	6.0
88	4.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1174083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.094973690882396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05228992090152	11.329123417964865
2	10.632949936752532	3.210081991034982
3	3.736168317106491	1.6919208735429254
4	1.7557212096782837	1.0601026187447162
5	1.0909101856987515	0.823363027611914
6	0.6861295307820299	0.6214264329415334
7	0.4948368653591902	0.5228684624712983
8	0.36200057450348744	0.43715113185715637
9	0.30233576452554256	0.41073753701932925
>10	3.294471686032517	11.78858007453176
>50	1.0517452756834604	11.202780223202984
>100	1.5075204814977172	52.94467090733607
>500	0.030082298764826435	2.8009735081408116
>1k	0.0028379527136628713	1.1562197935996652
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4748	0.40440071102298564	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4111	0.35014560299399616	No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT	1703	0.1450493704448493	TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	1487	0.1266520339703411	TruSeq Adapter, Index 23 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1446	0.1231599469543465	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15211871733088717	0.0
2	0.0	0.0	0.0	0.41606939202765053	0.0
3	0.0	0.0	0.0	0.5579673668726998	0.0
4	0.0	0.0	0.0	0.7362341503965222	0.0
5	0.0	0.0	0.0	1.2219749370359676	0.0
6	0.0	0.0	0.0	1.559940821901007	0.0
7	0.0	0.0	0.0	1.8197180267493867	0.0
8	0.0	0.0	0.0	2.4051962254798	0.0
9	0.0	0.0	0.0	2.6211945833471737	0.0
10	0.0	0.0	0.0	3.050039903482122	0.0
11	0.0	0.0	0.0	3.559799434963286	0.0
12	0.0	0.0	0.0	3.9713546657263583	0.0
13	0.0	0.0	0.0	4.1299465199649426	0.0
14	0.0	0.0	0.0	4.1802836767076945	0.0
15	0.0	0.0	0.0	4.281043163047246	0.0
16	1.7034570809729805E-4	0.0	0.0	4.506240189151875	0.0
17	1.7034570809729805E-4	0.0	0.0	4.781092989166865	0.0
18	1.7034570809729805E-4	0.0	0.0	5.234468091267823	0.0
19	1.7034570809729805E-4	0.0	0.0	5.404558280802975	0.0
20	1.7034570809729805E-4	0.0	0.0	5.597985832347457	0.0
21	1.7034570809729805E-4	0.0	0.0	5.857507518633692	0.0
22	1.7034570809729805E-4	0.0	0.0	6.103657066834287	0.0
23	1.7034570809729805E-4	0.0	0.0	6.4380456918292825	0.0
24	2.555185621459471E-4	0.0	0.0	6.642375368691992	0.0
25	3.406914161945961E-4	0.0	0.0	6.800626531514382	0.0
26	3.406914161945961E-4	0.0	0.0	6.981874364929907	0.0
27	3.406914161945961E-4	0.0	0.0	7.142425194811611	0.0
28	3.406914161945961E-4	0.0	0.0	7.3435183032204705	0.0
29	3.406914161945961E-4	0.0	0.0	7.515822986960888	0.0
30	3.406914161945961E-4	0.0	0.0	7.760013559518365	0.0
31	3.406914161945961E-4	0.0	0.0	7.990831993990203	0.0
32	3.406914161945961E-4	0.0	0.0	8.186218521177805	0.0
33	3.406914161945961E-4	0.0	0.0	8.406305176039513	0.0
34	4.2586427024324513E-4	0.0	0.0	8.63414256061965	0.0
35	4.2586427024324513E-4	0.0	0.0	8.901926013748602	0.0
36	4.2586427024324513E-4	0.0	0.0	9.11519884028642	0.0
37	4.2586427024324513E-4	0.0	0.0	9.32131714708415	0.0
38	4.2586427024324513E-4	0.0	0.0	9.52564682394686	0.0
39	4.2586427024324513E-4	0.0	0.0	9.716434017015834	0.0
40	4.2586427024324513E-4	0.0	0.0	9.957643539681607	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTGCG	75	0.0	46.000004	1
TAGCTAT	75	0.0	46.000004	11
TCACGAT	40	5.6115823E-9	46.0	12
TCACGAA	30	1.8614373E-6	46.0	22
CCTTCGC	20	6.31151E-4	46.0	42
TCTGCGA	30	1.8614373E-6	46.0	19
ACAACGT	45	3.092282E-10	46.0	30
AGCGTTA	30	1.8614373E-6	46.0	30
CGTGGTA	20	6.31151E-4	46.0	37
CTTCGAC	30	1.8614373E-6	46.0	23
TATCACG	30	1.8614373E-6	46.0	1
CGGAATT	20	6.31151E-4	46.0	14
AGTCCCG	20	6.31151E-4	46.0	24
TACCCGC	20	6.31151E-4	46.0	29
TCGCAAT	20	6.31151E-4	46.0	1
AAATCGA	20	6.31151E-4	46.0	30
TCGCAAG	55	1.8189894E-12	46.0	1
GCGACGC	20	6.31151E-4	46.0	32
TCCGCGA	20	6.31151E-4	46.0	30
CTCGTCT	20	6.31151E-4	46.0	19
>>END_MODULE