FastQCFastQC Report
Thu 26 May 2016
SRR1544723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544723_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1410337
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56920.4035914820358539No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52460.37196783463810423No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT19760.14010835708061264RNA PCR Primer, Index 38 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17910.12699092486405733No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT17260.12238209732851084RNA PCR Primer, Index 38 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG750.046.0000041
TGTTCGG1100.046.0000042
ATCGCCG551.8189894E-1246.0000041
TCGCCTA206.311945E-446.020
CGGGTAC301.8617066E-646.06
CTATGCG650.046.01
AACCGCA206.311945E-446.039
AATCCGG1000.046.02
ACCGGTA253.4175537E-546.011
CGAACTT253.4175537E-546.043
CTCCGAC453.110472E-1046.041
CACGGAT301.8617066E-646.029
TCCGATT253.4175537E-546.021
CAGCCGA301.8617066E-646.020
CGTGATA206.311945E-446.025
ACACCCG206.311945E-446.035
TTTCGCG600.046.01
GACGTGC301.8617066E-646.043
GACGTAT405.6134013E-946.09
TAGCGGA301.8617066E-646.03
Produced by FastQC (version 0.11.5)