##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544723_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1410337
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69872874355562	33.0	31.0	34.0	31.0	34.0
2	33.093291886974534	34.0	33.0	34.0	31.0	34.0
3	33.125544462068284	34.0	33.0	34.0	31.0	34.0
4	36.44504611309212	37.0	37.0	37.0	35.0	37.0
5	36.43852355855373	37.0	37.0	37.0	35.0	37.0
6	36.51233924941344	37.0	37.0	37.0	35.0	37.0
7	36.63401229635186	37.0	37.0	37.0	35.0	37.0
8	36.6318504017125	37.0	37.0	37.0	35.0	37.0
9	38.3702661137019	39.0	39.0	39.0	37.0	39.0
10	38.23095331115896	39.0	39.0	39.0	37.0	39.0
11	37.962962043823566	39.0	38.0	39.0	35.0	39.0
12	36.926576414006014	39.0	35.0	39.0	35.0	39.0
13	36.77116391330583	39.0	35.0	39.0	35.0	39.0
14	37.76672242166234	40.0	35.0	41.0	35.0	41.0
15	37.846293474538356	40.0	35.0	41.0	35.0	41.0
16	37.93818215079091	40.0	35.0	41.0	35.0	41.0
17	37.91374260194549	40.0	35.0	41.0	35.0	41.0
18	37.9676864465727	39.0	36.0	41.0	35.0	41.0
19	37.96506366917978	39.0	36.0	41.0	35.0	41.0
20	37.777299326331224	39.0	35.0	41.0	35.0	41.0
21	37.68235038859507	39.0	35.0	41.0	35.0	41.0
22	37.61814445767217	39.0	35.0	41.0	35.0	41.0
23	37.57499661428439	39.0	35.0	41.0	35.0	41.0
24	37.503897295469095	38.0	35.0	41.0	35.0	41.0
25	37.495882898909976	38.0	35.0	41.0	35.0	41.0
26	37.44156892997915	38.0	35.0	41.0	35.0	41.0
27	37.42471054790451	38.0	35.0	41.0	35.0	41.0
28	37.47027979837443	38.0	35.0	41.0	35.0	41.0
29	37.45313779614376	38.0	35.0	41.0	35.0	41.0
30	37.4274425190575	38.0	35.0	41.0	35.0	41.0
31	37.27566744685845	38.0	35.0	41.0	35.0	41.0
32	37.15033853610875	38.0	35.0	41.0	34.0	41.0
33	37.02651564838759	38.0	35.0	41.0	34.0	41.0
34	36.91059441821352	38.0	35.0	41.0	34.0	41.0
35	36.79121302213584	38.0	35.0	41.0	34.0	41.0
36	36.71335645310305	38.0	35.0	41.0	34.0	41.0
37	36.6601954001065	38.0	35.0	41.0	33.0	41.0
38	36.57544544318131	38.0	35.0	41.0	33.0	41.0
39	36.6227830653241	37.0	35.0	41.0	33.0	41.0
40	36.45444812126463	37.0	35.0	41.0	32.0	41.0
41	36.497313762597166	37.0	35.0	41.0	33.0	41.0
42	36.49141162715011	37.0	35.0	41.0	33.0	41.0
43	36.43972823516649	37.0	35.0	41.0	33.0	41.0
44	36.350684978129344	37.0	35.0	41.0	33.0	41.0
45	36.30117907989367	37.0	35.0	41.0	33.0	41.0
46	36.25618345118932	36.0	35.0	41.0	33.0	41.0
47	36.19806897216765	36.0	35.0	40.0	33.0	41.0
48	36.157777183751115	36.0	35.0	40.0	33.0	41.0
49	36.18793593304295	36.0	35.0	40.0	33.0	41.0
50	36.04196372923634	36.0	35.0	40.0	33.0	41.0
51	35.979050397174575	36.0	35.0	40.0	33.0	41.0
52	35.72078304688879	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	1.0
14	5.0
15	13.0
16	31.0
17	89.0
18	169.0
19	367.0
20	658.0
21	1113.0
22	1802.0
23	2939.0
24	5000.0
25	7969.0
26	10389.0
27	11660.0
28	11454.0
29	11783.0
30	13061.0
31	15525.0
32	20607.0
33	29509.0
34	84839.0
35	410757.0
36	50616.0
37	57298.0
38	117762.0
39	542312.0
40	2606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.5512271180576	23.974269979444628	29.687088972352	13.787413930145773
2	30.003254541290485	25.29778343757556	31.66491413045251	13.034047890681446
3	28.25686343051342	25.917138953313994	32.02830245537059	13.797695160801993
4	25.52865024458693	28.291323279471502	32.098782064144956	14.081244411796611
5	23.40972405885969	33.8914032603555	28.247078535130253	14.451794145654548
6	21.863356063125337	42.80459209394634	24.79655571682513	10.535496126103194
7	89.339640100203	4.14936288277199	4.9724994806205896	1.5384975364044196
8	89.95913742601945	2.9604980937180265	5.17053725457107	1.9098272256914481
9	85.9905823927189	4.683703256739347	6.730589922834046	2.595124427707704
10	56.09028196806862	25.698680528129092	10.210183807132621	8.000853696669662
11	49.86545768848155	17.513402824998565	20.796235226048807	11.82490426047108
12	47.32266117956205	20.093424479397477	21.46274259272784	11.121171748312637
13	19.273478608304256	47.82360528015644	21.85491836348334	11.04799774805596
14	11.908217681305958	50.41901332802018	25.16958712704836	12.503181863625501
15	10.831808284119328	22.904809276080822	53.790122502635896	12.473259937163954
16	12.269407950014784	18.378727921057166	51.964246843130404	17.38761728579765
17	13.034260605798472	18.067596609888277	29.106447607912152	39.791695176401106
18	17.332949500722165	23.832388996388808	39.966759717712854	18.867901785176166
19	29.486924047231266	23.039883375391838	27.28978960347775	20.183402973899145
20	32.21095383585625	21.98977974767733	27.479460582825237	18.31980583364118
21	18.303568579708255	31.174889405865407	31.811333036004868	18.71020897842147
22	20.553739992640057	23.5607517919476	23.42454321201245	32.46096500339989
23	14.624164295483988	32.27951900857738	25.065427624745006	28.03088907119362
24	14.941109819851567	22.43527610776715	47.35229948586756	15.271314586513721
25	12.219207182396831	26.289177692991107	41.406770154934605	20.08484496967746
26	12.94889093883235	37.814579068690676	28.967615541533693	20.268914450943285
27	13.531943074598482	42.78558954349209	27.148334050655976	16.534133331253454
28	11.445704111854118	32.84399402412331	40.121758132985235	15.588543731037333
29	11.914740944894731	23.527993663925713	39.01450504382995	25.542760347349603
30	15.419080687807242	36.89919501509214	30.86964321293421	16.812081084166408
31	29.17891255777874	30.42322508733728	25.97251578877956	14.425346566104412
32	30.390537864354407	24.90674214744419	29.00994584982171	15.692774138379692
33	31.168011617081593	28.851685802754943	22.970680057319633	17.00962252284383
34	19.144785962503995	27.555328974564237	26.234013572642567	27.0658714902892
35	17.187523265715924	25.600051618868395	33.53489272422123	23.677532391194443
36	33.91777993486663	25.064293144120875	25.13895615019673	15.87897077081577
37	16.44401302667377	38.733366564161614	30.0902550241538	14.732365385010818
38	17.28203968271413	37.1460154558804	24.84228946698555	20.729655394419915
39	19.293899259538676	36.72803025092584	27.700117064219405	16.277953425316078
40	25.744201563172492	25.62820091935474	26.18778348720909	22.439814030263687
41	15.174671018345261	25.29891791819969	29.46097280295419	30.06543826050086
42	19.787185615920166	22.53454316237892	26.08433303529582	31.59393818640509
43	20.77333289844909	23.15524587385852	28.43923119084304	27.632190036849348
44	15.124612060805326	30.85737664118576	32.00710184870708	22.01090944930183
45	13.577676824758905	45.825997616172586	21.771817657765485	18.824507901303022
46	20.209212408098207	37.20366125259424	25.597144512269054	16.989981827038502
47	19.007442901944714	29.474161140209752	26.220116184996918	25.29827977284862
48	20.724904756806353	25.268925086699134	35.4103310059936	18.595839150500908
49	22.408899433256025	24.078358576708972	35.21108784637998	18.30165414365503
50	18.153958947400515	37.40609513896324	27.212503110958586	17.227442802677658
51	16.28227863269559	35.17265731523742	25.65869008612835	22.886373965938635
52	18.098723922012965	26.875136935356586	37.86258178009936	17.163557362531083
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6229.0
1	5203.0
2	4177.0
3	6545.5
4	8914.0
5	6102.0
6	3290.0
7	3526.0
8	3762.0
9	4194.0
10	4626.0
11	4667.5
12	4709.0
13	4682.0
14	4396.5
15	4138.0
16	4078.5
17	4019.0
18	4206.0
19	4393.0
20	4719.5
21	5046.0
22	4988.0
23	4930.0
24	5726.5
25	6523.0
26	8445.0
27	10367.0
28	11565.5
29	12764.0
30	18160.0
31	23556.0
32	29024.5
33	34493.0
34	38312.5
35	42132.0
36	46110.5
37	50089.0
38	54053.0
39	81526.5
40	105036.0
41	134354.0
42	163672.0
43	182049.0
44	200426.0
45	183498.0
46	166570.0
47	152671.5
48	138773.0
49	122716.0
50	106659.0
51	89835.0
52	73011.0
53	61662.5
54	50314.0
55	42096.0
56	33878.0
57	27942.0
58	22006.0
59	19130.0
60	16254.0
61	13643.5
62	11033.0
63	9395.0
64	6647.0
65	5537.0
66	4131.0
67	2725.0
68	2250.0
69	1775.0
70	1520.0
71	1265.0
72	1346.5
73	1428.0
74	1050.0
75	672.0
76	531.0
77	390.0
78	296.5
79	203.0
80	110.0
81	17.0
82	55.0
83	93.0
84	50.0
85	7.0
86	5.0
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1410337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.640309969725326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96948886721421	10.829362453181627
2	11.179633759397314	3.2734660717116464
3	4.15023868696819	1.8228234247668043
4	2.0190237213705178	1.182365324683709
5	1.181882084028453	0.8651560028920752
6	0.7520959635969693	0.6606550820423331
7	0.5163736156329455	0.5291908855137888
8	0.39111249119043145	0.45808064832475054
9	0.31489997871695086	0.4149209968088461
>10	2.969657991050935	10.040345403671877
>50	0.981308671390317	10.300769059557043
>100	1.525072562784267	53.91593242242395
>500	0.044339170355708724	4.116327187858515
>1k	0.0038979490422601077	0.8103539875933233
>5k	9.744872605650269E-4	0.7802510489696982
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5692	0.4035914820358539	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5246	0.37196783463810423	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT	1976	0.14010835708061264	RNA PCR Primer, Index 38 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1791	0.12699092486405733	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1726	0.12238209732851084	RNA PCR Primer, Index 38 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14599347531831044	0.0
2	0.0	0.0	0.0	0.4641443853490336	0.0
3	0.0	0.0	0.0	0.6399888820898835	0.0
4	0.0	0.0	0.0	0.851994948725021	0.0
5	0.0	0.0	0.0	1.4529860593602806	0.0
6	0.0	0.0	0.0	1.87458742130427	0.0
7	0.0	0.0	0.0	2.1638799804585713	0.0
8	0.0	0.0	0.0	2.80897402535706	0.0
9	0.0	0.0	0.0	3.0182147954708696	0.0
10	0.0	0.0	0.0	3.4309530275388083	0.0
11	7.090503900840721E-5	0.0	0.0	3.9682005081055096	0.0
12	7.090503900840721E-5	0.0	0.0	4.386823858411145	0.0
13	7.090503900840721E-5	0.0	0.0	4.544162139970801	0.0
14	7.090503900840721E-5	0.0	0.0	4.596135533563964	0.0
15	7.090503900840721E-5	0.0	0.0	4.697175214150944	0.0
16	7.090503900840721E-5	0.0	0.0	4.947399096811613	0.0
17	7.090503900840721E-5	0.0	0.0	5.244633020334856	0.0
18	7.090503900840721E-5	0.0	0.0	5.685520552889132	0.0
19	7.090503900840721E-5	0.0	0.0	5.869660939193966	0.0
20	1.4181007801681442E-4	0.0	0.0	6.0487670677292025	0.0
21	2.8362015603362883E-4	0.0	0.0	6.318135310922141	0.0
22	2.8362015603362883E-4	0.0	0.0	6.5777186587319205	0.0
23	2.8362015603362883E-4	0.0	0.0	6.909057906018207	0.0
24	2.8362015603362883E-4	0.0	0.0	7.152474904934069	0.0
25	2.8362015603362883E-4	0.0	0.0	7.332999134249474	0.0
26	2.8362015603362883E-4	0.0	0.0	7.529689712458795	0.0
27	2.8362015603362883E-4	0.0	0.0	7.708937651072048	0.0
28	2.8362015603362883E-4	0.0	0.0	7.892652607142832	0.0
29	3.5452519504203603E-4	0.0	0.0	8.094093822965716	0.0
30	3.5452519504203603E-4	0.0	0.0	8.374735967360992	0.0
31	3.5452519504203603E-4	0.0	0.0	8.640417148525493	0.0
32	3.5452519504203603E-4	0.0	0.0	8.859230098905439	0.0
33	4.254302340504433E-4	0.0	0.0	9.070456210111484	0.0
34	4.254302340504433E-4	0.0	0.0	9.296217854314252	0.0
35	4.254302340504433E-4	0.0	0.0	9.606214684859008	0.0
36	4.254302340504433E-4	0.0	0.0	9.8549495617005	0.0
37	4.254302340504433E-4	0.0	0.0	10.087730804765103	0.0
38	4.254302340504433E-4	0.0	0.0	10.323560964507065	0.0
39	4.963352730588505E-4	0.0	0.0	10.531383633840706	0.0
40	4.963352730588505E-4	0.0	0.0	10.796781194849174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	75	0.0	46.000004	1
TGTTCGG	110	0.0	46.000004	2
ATCGCCG	55	1.8189894E-12	46.000004	1
TCGCCTA	20	6.311945E-4	46.0	20
CGGGTAC	30	1.8617066E-6	46.0	6
CTATGCG	65	0.0	46.0	1
AACCGCA	20	6.311945E-4	46.0	39
AATCCGG	100	0.0	46.0	2
ACCGGTA	25	3.4175537E-5	46.0	11
CGAACTT	25	3.4175537E-5	46.0	43
CTCCGAC	45	3.110472E-10	46.0	41
CACGGAT	30	1.8617066E-6	46.0	29
TCCGATT	25	3.4175537E-5	46.0	21
CAGCCGA	30	1.8617066E-6	46.0	20
CGTGATA	20	6.311945E-4	46.0	25
ACACCCG	20	6.311945E-4	46.0	35
TTTCGCG	60	0.0	46.0	1
GACGTGC	30	1.8617066E-6	46.0	43
GACGTAT	40	5.6134013E-9	46.0	9
TAGCGGA	30	1.8617066E-6	46.0	3
>>END_MODULE