FastQCFastQC Report
Thu 26 May 2016
SRR1544722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544722_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1546212
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62240.40253212366738844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61020.3946418731713374No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT43150.27906910565950854Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT33750.21827537232927954No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24320.157287616445869No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT22410.14493484722664163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG301.861823E-646.01
TATCACG253.4177065E-546.01
ATCTCGA301.861823E-646.020
CAAACGT253.4177065E-546.045
CGAACAC206.312135E-446.015
GCGACTA551.8189894E-1246.046
CGTATTA405.6134013E-946.010
GTCGACG301.861823E-646.025
TTTACTC253.4177065E-546.031
CGAGTCT206.312135E-446.014
CGACGTG301.861823E-646.027
GTTTCGA405.6134013E-946.034
ACGCATG405.6134013E-946.01
TTAGCGA206.312135E-446.028
ACCGCGT301.861823E-646.027
TAGGTCG253.4177065E-546.041
CGCATCG206.312135E-446.021
ACCGAAC253.4177065E-546.011
GCGCCTA206.312135E-446.010
GCGCCCA206.312135E-446.09
Produced by FastQC (version 0.11.5)