##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544722_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1546212
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.645921128538646	33.0	31.0	34.0	31.0	34.0
2	33.03647947370736	34.0	33.0	34.0	31.0	34.0
3	33.076100172550724	34.0	33.0	34.0	31.0	34.0
4	36.40874343233657	37.0	37.0	37.0	35.0	37.0
5	36.39582541074574	37.0	37.0	37.0	35.0	37.0
6	36.46754455404563	37.0	37.0	37.0	35.0	37.0
7	36.598506543733976	37.0	37.0	37.0	35.0	37.0
8	36.58978522996846	37.0	37.0	37.0	35.0	37.0
9	38.320029853603515	39.0	39.0	39.0	37.0	39.0
10	38.185482973874215	39.0	39.0	39.0	37.0	39.0
11	37.93542476710826	39.0	38.0	39.0	35.0	39.0
12	37.04682022905009	39.0	35.0	39.0	35.0	39.0
13	36.90091203534832	39.0	35.0	39.0	35.0	39.0
14	37.93720524740463	40.0	35.0	41.0	35.0	41.0
15	37.99086347797068	40.0	35.0	41.0	35.0	41.0
16	38.08361854648651	40.0	35.0	41.0	35.0	41.0
17	38.064645727752726	40.0	35.0	41.0	35.0	41.0
18	38.123907329654664	40.0	36.0	41.0	35.0	41.0
19	38.08703010971329	40.0	36.0	41.0	35.0	41.0
20	37.911647949957704	40.0	35.0	41.0	35.0	41.0
21	37.83280559198868	40.0	35.0	41.0	35.0	41.0
22	37.7585356988563	39.0	35.0	41.0	35.0	41.0
23	37.70880060431558	39.0	35.0	41.0	35.0	41.0
24	37.65420718504319	39.0	35.0	41.0	35.0	41.0
25	37.65452473528856	39.0	35.0	41.0	35.0	41.0
26	37.59986728857363	39.0	35.0	41.0	35.0	41.0
27	37.57176894242187	39.0	35.0	41.0	35.0	41.0
28	37.58282887469506	39.0	35.0	41.0	35.0	41.0
29	37.57768080961731	39.0	35.0	41.0	35.0	41.0
30	37.5282593848709	39.0	35.0	41.0	35.0	41.0
31	37.3902446753744	39.0	35.0	41.0	34.0	41.0
32	37.2454385297747	39.0	35.0	41.0	34.0	41.0
33	37.11568788756005	39.0	35.0	41.0	34.0	41.0
34	36.99728368425546	39.0	35.0	41.0	34.0	41.0
35	36.87981143594798	38.0	35.0	41.0	34.0	41.0
36	36.801795614055514	38.0	35.0	41.0	33.0	41.0
37	36.778235455422674	38.0	35.0	41.0	33.0	41.0
38	36.66471027258875	38.0	35.0	41.0	33.0	41.0
39	36.700109687416735	38.0	35.0	41.0	33.0	41.0
40	36.600967396450166	38.0	35.0	41.0	33.0	41.0
41	36.59365274619522	38.0	35.0	41.0	33.0	41.0
42	36.59103732217833	38.0	35.0	41.0	33.0	41.0
43	36.53315974782242	38.0	35.0	41.0	33.0	41.0
44	36.432377319539626	38.0	35.0	41.0	33.0	41.0
45	36.352717479879864	38.0	35.0	41.0	33.0	41.0
46	36.3057342718851	37.0	35.0	41.0	33.0	41.0
47	36.2477758547987	37.0	35.0	41.0	33.0	41.0
48	36.233561115810765	37.0	35.0	41.0	33.0	41.0
49	36.2664634603793	37.0	35.0	40.0	33.0	41.0
50	36.11599897038698	37.0	35.0	40.0	33.0	41.0
51	36.05748047486373	37.0	35.0	40.0	33.0	41.0
52	35.850656313623226	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	5.0
14	17.0
15	28.0
16	57.0
17	132.0
18	232.0
19	425.0
20	823.0
21	1324.0
22	2090.0
23	3422.0
24	5674.0
25	9091.0
26	12009.0
27	13237.0
28	13461.0
29	13834.0
30	15284.0
31	18222.0
32	23575.0
33	33349.0
34	87456.0
35	396946.0
36	65186.0
37	70595.0
38	135383.0
39	621352.0
40	2996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.822609060077145	24.224879900039582	30.067028324705795	13.88548271517748
2	29.827345797342147	25.818581151873094	30.234405113917106	14.119667936867646
3	28.267857189052986	25.298406686793275	32.429576280613524	14.004159843540211
4	25.02871533787087	28.509221245210874	31.62793976505162	14.834123651866626
5	22.173414771066323	32.873176511371014	30.157442834488414	14.795965883074249
6	21.80716486484389	41.99689305218172	25.07786771801021	11.118074364964183
7	87.70175111821665	4.2287862207769695	6.06870209259791	2.000760568408472
8	87.70369134374847	3.1841687944473334	6.166683481954609	2.9454563798495936
9	83.55568317927943	4.634422705295264	8.337731177872115	3.472162937553194
10	52.17305259563372	24.815290529371133	12.180412517817738	10.831244357177413
11	46.430308392380866	19.540981443683016	21.363693982455185	12.665016181480935
12	42.53375345683516	20.606035912281108	24.433518818894175	12.426691811989558
13	19.57558213233373	44.163284206822865	23.816850470698714	12.44428319014469
14	12.994143105861292	45.826574881064175	27.60151906724304	13.57776294583149
15	10.932588804122592	24.0256187379221	51.421344550423875	13.620447907531439
16	13.607513070652665	20.072215194294184	48.764787752261654	17.555483982791493
17	12.952169560189677	19.442288638297985	30.360067054194378	37.245474747317964
18	18.231135187154155	22.52265536679317	39.77992668534457	19.466282760708104
19	28.461944416418966	23.6695226786495	27.37140831916969	20.49712458576185
20	30.298755927389	22.669659787920416	28.435622023370666	18.595962261319922
21	19.4863317578702	31.238665849184976	30.09238060498819	19.182621787956634
22	19.272971623554856	23.92614984232434	26.366953561348637	30.433924972772168
23	15.206129560500111	31.928739396667467	26.57630389623156	26.288827146600852
24	15.282509772269263	22.874547604080163	44.29631900412104	17.546623619529534
25	13.605378822567667	25.37103579586758	40.39704775283079	20.626537628733963
26	13.301086784994553	36.767661873016124	28.464466709610324	21.466784632379003
27	13.696504748378619	40.0956660535554	28.569174214143985	17.638654983922
28	12.211844171433155	30.282587381290533	39.65245386790427	17.85311457937204
29	12.497186672978868	24.293240512943893	38.72657824412176	24.48299456995548
30	16.082270736483743	35.108639694944806	31.151355700253262	17.657733868318186
31	28.061287844099	28.156229546789184	26.586263720628217	17.196218888483596
32	29.942142474641255	24.391480599038164	28.589029188752903	17.07734773756768
33	27.401158443990862	27.55049113575629	25.76153852123771	19.28681189901514
34	16.47697728383947	26.579925650557623	28.56775138208732	28.375345683515583
35	16.64474211815715	24.015076845865895	35.063755810975465	24.27642522500149
36	29.974544241022578	24.69460850129219	27.740762586243022	17.590084671442206
37	16.97981906750174	34.592863074403766	31.198891225782756	17.228426632311738
38	16.08408161364677	35.94131981901576	26.304931018514928	21.66966754882254
39	18.549849567847097	33.72648770026361	28.549642610457038	19.174020121432246
40	24.603870620587603	24.445806913929008	27.043186833370847	23.907135632112546
41	14.524463656988823	23.995480567994555	29.59665298160925	31.883402793407374
42	18.39353206416714	22.57827516537189	28.36609727514726	30.662095495313707
43	19.02611026172349	23.10414095867837	30.80379663332066	27.065952146277482
44	16.036416739748496	28.878575512284215	32.250687486580105	22.834320261387184
45	14.69882525811467	40.17540932291303	24.699200368384155	20.426565050588145
46	19.754794297289116	34.8147602010591	26.283006470005404	19.14743903164637
47	17.137171358132004	27.600160909370775	28.36926631018256	26.89340142231466
48	18.145829937938654	24.011519765724234	36.563226776147125	21.279423520189987
49	20.88930883992622	23.066306560807963	36.53709840565201	19.50728619361381
50	18.205718232687367	34.91914433467079	27.953217281976855	18.92192015066498
51	15.881392719756413	33.52147053573508	26.104376372709563	24.49276037179895
52	18.152038659640464	25.522890780824365	36.62725421869705	19.697816340838127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7010.0
1	6165.0
2	5320.0
3	8250.0
4	11180.0
5	7663.0
6	4146.0
7	4560.0
8	4974.0
9	5177.0
10	5380.0
11	5607.5
12	5835.0
13	6046.0
14	6083.5
15	5910.0
16	5477.5
17	5045.0
18	4922.0
19	4799.0
20	4102.5
21	3406.0
22	4645.5
23	5885.0
24	6999.5
25	8114.0
26	9805.5
27	11497.0
28	14385.0
29	17273.0
30	20689.0
31	24105.0
32	26826.0
33	29547.0
34	34371.0
35	39195.0
36	43143.5
37	47092.0
38	54990.5
39	87508.0
40	112127.0
41	141770.0
42	171413.0
43	189031.5
44	206650.0
45	206112.5
46	205575.0
47	176387.5
48	147200.0
49	128236.0
50	109272.0
51	97757.5
52	86243.0
53	72205.0
54	58167.0
55	49328.0
56	40489.0
57	34909.5
58	29330.0
59	25029.0
60	20728.0
61	18129.0
62	15530.0
63	13113.0
64	8407.5
65	6119.0
66	5231.0
67	4343.0
68	3433.5
69	2524.0
70	2239.0
71	1954.0
72	1683.0
73	1412.0
74	1090.0
75	768.0
76	614.5
77	461.0
78	343.5
79	226.0
80	154.0
81	82.0
82	55.5
83	29.0
84	17.5
85	6.0
86	6.0
87	6.0
88	4.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1546212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.371704803009308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.81099872355496	11.756684727113198
2	11.683765079179944	3.8256630572808534
3	4.664316271938814	2.2908842733616543
4	2.488915721421542	1.6299117388272975
5	1.464951556015202	1.1991877212895021
6	0.9423560090333657	0.925678463954174
7	0.6963913822376128	0.7980779896147671
8	0.47704320679793955	0.6248008447981431
9	0.39324368939827115	0.5794262638627299
>10	3.2227350315426855	11.698414773440744
>50	0.8571787854234242	10.042797292958898
>100	1.2614933791405012	49.17105262811023
>500	0.03183579505717353	3.333048746634865
>1k	0.003979474382146691	1.3213229662523052
>5k	7.958948764293382E-4	0.8030485125006614
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6224	0.40253212366738844	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6102	0.3946418731713374	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT	4315	0.27906910565950854	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	3375	0.21827537232927954	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2432	0.157287616445869	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	2241	0.14493484722664163	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.467418439386061E-5	0.0	0.0	0.28986969445328326	0.0
2	6.467418439386061E-5	0.0	0.0	0.8502715022260854	0.0
3	6.467418439386061E-5	0.0	0.0	1.135355307034223	0.0
4	6.467418439386061E-5	0.0	0.0	1.4942970304201493	0.0
5	6.467418439386061E-5	0.0	0.0	2.4789614878166772	0.0
6	6.467418439386061E-5	0.0	0.0	3.185074233028847	0.0
7	6.467418439386061E-5	0.0	0.0	3.6546088117282753	0.0
8	6.467418439386061E-5	0.0	0.0	4.696251225575795	0.0
9	6.467418439386061E-5	0.0	0.0	5.025960217615696	0.0
10	6.467418439386061E-5	0.0	0.0	5.666946059143248	0.0
11	6.467418439386061E-5	0.0	0.0	6.479383163498925	0.0
12	6.467418439386061E-5	0.0	0.0	7.113772238218304	0.0
13	6.467418439386061E-5	0.0	0.0	7.363414589978606	0.0
14	6.467418439386061E-5	0.0	0.0	7.441670353095177	0.0
15	6.467418439386061E-5	0.0	0.0	7.591908483442115	0.0
16	6.467418439386061E-5	0.0	0.0	7.9471637783175915	0.0
17	6.467418439386061E-5	0.0	0.0	8.388629760990083	0.0
18	1.2934836878772122E-4	0.0	0.0	9.021531329468404	0.0
19	1.2934836878772122E-4	0.0	0.0	9.290705284915651	0.0
20	1.2934836878772122E-4	0.0	0.0	9.582967924191507	0.0
21	1.2934836878772122E-4	0.0	0.0	9.964739634668467	0.0
22	1.2934836878772122E-4	0.0	0.0	10.354724966563447	0.0
23	1.2934836878772122E-4	0.0	0.0	10.840557439730128	0.0
24	1.9402255318158183E-4	0.0	0.0	11.15403321148717	0.0
25	1.9402255318158183E-4	0.0	0.0	11.409431565658526	0.0
26	1.9402255318158183E-4	0.0	0.0	11.680998465928345	0.0
27	1.9402255318158183E-4	0.0	0.0	11.926048950596684	0.0
28	1.9402255318158183E-4	0.0	0.0	12.22568444689344	0.0
29	1.9402255318158183E-4	0.0	0.0	12.503977462340222	0.0
30	1.9402255318158183E-4	0.0	0.0	12.87132682969735	0.0
31	2.5869673757544244E-4	0.0	0.0	13.213970658616024	0.0
32	2.5869673757544244E-4	0.0	0.0	13.522789889096709	0.0
33	2.5869673757544244E-4	0.0	0.0	13.840016763548595	0.0
34	3.2337092196930306E-4	0.0	0.0	14.154009928780788	0.0
35	3.2337092196930306E-4	0.0	0.0	14.54955724053364	0.0
36	3.2337092196930306E-4	0.0	0.0	14.869306408176886	0.0
37	3.2337092196930306E-4	0.0	0.0	15.185757192416046	0.0
38	3.2337092196930306E-4	0.0	0.0	15.484875295237652	0.0
39	3.8804510636316367E-4	0.0	0.0	15.809216329972863	0.0
40	3.8804510636316367E-4	0.0	0.0	16.154317777898502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	30	1.861823E-6	46.0	1
TATCACG	25	3.4177065E-5	46.0	1
ATCTCGA	30	1.861823E-6	46.0	20
CAAACGT	25	3.4177065E-5	46.0	45
CGAACAC	20	6.312135E-4	46.0	15
GCGACTA	55	1.8189894E-12	46.0	46
CGTATTA	40	5.6134013E-9	46.0	10
GTCGACG	30	1.861823E-6	46.0	25
TTTACTC	25	3.4177065E-5	46.0	31
CGAGTCT	20	6.312135E-4	46.0	14
CGACGTG	30	1.861823E-6	46.0	27
GTTTCGA	40	5.6134013E-9	46.0	34
ACGCATG	40	5.6134013E-9	46.0	1
TTAGCGA	20	6.312135E-4	46.0	28
ACCGCGT	30	1.861823E-6	46.0	27
TAGGTCG	25	3.4177065E-5	46.0	41
CGCATCG	20	6.312135E-4	46.0	21
ACCGAAC	25	3.4177065E-5	46.0	11
GCGCCTA	20	6.312135E-4	46.0	10
GCGCCCA	20	6.312135E-4	46.0	9
>>END_MODULE