FastQCFastQC Report
Thu 26 May 2016
SRR1544721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544721_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2070810
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86680.4185801691125695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33950.1639455092451746No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT28600.1381102080828275Illumina Single End Adapter 2 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT351.02010745E-746.00000414
TCGCATA206.3126325E-446.035
CGAACTA253.4181117E-546.033
TACCCGG206.3126325E-446.017
CGAACCG253.4181117E-546.020
GTCGACC206.3126325E-446.040
CGAAACG206.3126325E-446.026
CGTGAAT253.4181117E-546.034
ATAGATC501.6370905E-1146.043
TGCGATT405.6152203E-946.017
TCGCTAA206.3126325E-446.029
CTCGACC206.3126325E-446.031
CGGTCTA4250.043.83529331
CTAACGG1600.043.1252
TTTCGCG750.042.933331
TTAATCG5350.042.56074520
CTAGACG650.042.461541
TAATCGT5400.042.16666821
GGCGATA6450.042.0775228
TCACGAC4550.041.95604325
Produced by FastQC (version 0.11.5)