##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544721_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2070810
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64036101815232	33.0	31.0	34.0	31.0	34.0
2	33.069421144383114	34.0	33.0	34.0	31.0	34.0
3	33.136251998010444	34.0	33.0	34.0	31.0	34.0
4	36.46518946692357	37.0	37.0	37.0	35.0	37.0
5	36.45770930215713	37.0	37.0	37.0	35.0	37.0
6	36.526429754540494	37.0	37.0	37.0	35.0	37.0
7	36.612968355377845	37.0	37.0	37.0	35.0	37.0
8	36.609665300051674	37.0	37.0	37.0	35.0	37.0
9	38.30451755593222	39.0	39.0	39.0	37.0	39.0
10	38.22266311250187	39.0	39.0	39.0	37.0	39.0
11	38.12577542121199	39.0	39.0	39.0	35.0	39.0
12	37.58272415141901	39.0	37.0	39.0	35.0	39.0
13	37.46683519975275	39.0	37.0	39.0	35.0	39.0
14	38.6677691338172	41.0	37.0	41.0	35.0	41.0
15	38.71112366658458	41.0	37.0	41.0	35.0	41.0
16	38.78188052018292	41.0	37.0	41.0	35.0	41.0
17	38.745404455261465	40.0	37.0	41.0	35.0	41.0
18	38.76192842414321	40.0	36.0	41.0	35.0	41.0
19	38.74815748426944	40.0	36.0	41.0	35.0	41.0
20	38.6389441812624	40.0	36.0	41.0	35.0	41.0
21	38.58340697601422	40.0	35.0	41.0	35.0	41.0
22	38.55093900454411	40.0	35.0	41.0	35.0	41.0
23	38.468446163578506	40.0	35.0	41.0	35.0	41.0
24	38.43130320985508	40.0	35.0	41.0	35.0	41.0
25	38.41703584587673	40.0	35.0	41.0	35.0	41.0
26	38.36539083740179	40.0	35.0	41.0	35.0	41.0
27	38.32585316856689	40.0	35.0	41.0	35.0	41.0
28	38.28758939738557	40.0	36.0	41.0	35.0	41.0
29	38.25453180156557	40.0	36.0	41.0	35.0	41.0
30	38.204661943877035	40.0	36.0	41.0	35.0	41.0
31	38.11138878023575	40.0	35.0	41.0	35.0	41.0
32	38.02831983619936	40.0	35.0	41.0	35.0	41.0
33	37.90898392416494	40.0	35.0	41.0	35.0	41.0
34	37.763964825358194	40.0	35.0	41.0	34.0	41.0
35	37.6536809267871	40.0	35.0	41.0	34.0	41.0
36	37.581565184637896	40.0	35.0	41.0	34.0	41.0
37	37.542228886281215	40.0	35.0	41.0	34.0	41.0
38	37.40767380879946	40.0	35.0	41.0	33.0	41.0
39	37.407930230199774	40.0	35.0	41.0	34.0	41.0
40	37.321254484959994	40.0	35.0	41.0	33.0	41.0
41	37.2757225433526	40.0	35.0	41.0	34.0	41.0
42	37.26543236704478	40.0	35.0	41.0	34.0	41.0
43	37.21213969412935	40.0	35.0	41.0	33.0	41.0
44	37.115804926574626	40.0	35.0	41.0	33.0	41.0
45	37.04024898469681	40.0	35.0	41.0	33.0	41.0
46	36.995823856365384	39.0	35.0	41.0	33.0	41.0
47	36.93473520023566	39.0	35.0	41.0	33.0	41.0
48	36.87945393348497	39.0	35.0	41.0	33.0	41.0
49	36.83973469318769	39.0	35.0	41.0	33.0	41.0
50	36.73383989839724	39.0	35.0	41.0	33.0	41.0
51	36.66707568535983	39.0	35.0	41.0	33.0	41.0
52	36.45888758505126	38.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	5.0
14	9.0
15	19.0
16	44.0
17	120.0
18	272.0
19	626.0
20	992.0
21	1488.0
22	2162.0
23	3690.0
24	5886.0
25	9625.0
26	13874.0
27	16289.0
28	16472.0
29	16835.0
30	18205.0
31	21824.0
32	28092.0
33	39018.0
34	94720.0
35	321699.0
36	85927.0
37	114925.0
38	232008.0
39	1022741.0
40	3237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.79387775797876	23.3894466416523	28.85711388297333	13.959561717395609
2	30.256566271169255	25.355971817791108	30.713633795471335	13.673828115568304
3	28.97088578865275	25.153297501943683	31.71816825300245	14.157648456401118
4	25.853458308584564	28.23880510524867	31.160608650721215	14.747127935445551
5	22.72965651122025	34.28117499915492	28.724508767100797	14.264659722524037
6	22.033841830008548	42.768530188670134	24.900449582530506	10.29717839879081
7	89.36672123468594	3.344633259449201	5.539668052597776	1.748977453267079
8	89.39019031200351	2.6527783814063097	5.929998406420676	2.0270329001694987
9	83.63340914907693	5.976260497100169	7.436800092717342	2.953530261105558
10	37.78704951202669	37.92260033513456	13.287264403784027	11.003085749054717
11	37.84567391503808	21.588508844365247	24.375292759837937	16.190524480758736
12	36.70732708457077	20.817361322381096	26.765661745886877	15.709649847161256
13	23.521375693569183	32.6441344208305	27.860933644322756	15.973556241277567
14	17.747548060903704	34.50046117219832	30.24560437703121	17.50638638986677
15	16.312505734471053	25.63668323023358	41.568033764565556	16.482777270729812
16	20.86077428639035	22.07044586417875	39.098855037400824	17.969924812030076
17	19.90757239920611	22.112651571124342	29.730685094238485	28.249090935431063
18	21.26655753062811	23.24921166113743	35.89339437225047	19.590836435983988
19	25.57467850744395	24.855008426654305	29.473780791091407	20.09653227481034
20	27.17303856944867	25.554251717926803	28.73976849638547	18.532941216239056
21	21.988980157522903	27.024159628357985	31.792921610384344	19.193938603734768
22	21.923401953824833	22.101061903313195	29.756761846813568	26.21877429604841
23	17.94095064250221	27.582878197420335	30.391489320604016	24.084681839473443
24	17.85798793708742	24.37630685577141	38.67375568014448	19.091949526996682
25	18.602092900845562	25.999777864700285	34.7227413427596	20.675387891694555
26	17.623586905606984	31.800648055591772	28.75106842250134	21.824696616299903
27	16.161598601513415	33.28851995113023	30.307898841516124	20.241982605840228
28	15.628908494743603	28.702343527411976	35.929032600769744	19.73971537707467
29	17.424341199820358	24.802372018678682	35.24162042872113	22.53166635277983
30	18.945098777772948	30.00106238621602	31.765589310463056	19.288249525547975
31	24.947291156600556	27.29130147140491	28.51932335656096	19.242084015433576
32	26.232730187704327	25.14223902724055	30.099719433458404	18.52531135159672
33	25.41797654058074	26.410679878887972	27.67052506024213	20.50081852028916
34	19.606579068094128	26.468386766530973	29.623722118398117	24.301312046976786
35	18.67641164568454	26.43646688976777	32.98221468893814	21.904906775609547
36	27.22813778183416	25.783920301717682	28.2078510341364	18.780090882311754
37	18.870490291238696	32.768530188670134	29.31529208377398	19.04568743631719
38	19.176457521452956	31.585080234304453	27.91738498461955	21.321077259623046
39	19.04124473032292	30.711460732756745	28.695920919833302	21.551373617087034
40	23.276109348515796	25.08424239790227	29.61898967070856	22.020658582873367
41	17.896620163124574	24.84061792245546	30.232710871591312	27.030051042828653
42	19.501547703555612	24.6826121179635	29.89120199342286	25.92463818505802
43	19.604212844249354	25.013110811711357	30.02279301336192	25.35988333067737
44	17.673857089737833	27.613735687967512	32.69324563818023	22.01916158411443
45	17.529324274076327	33.6860938473351	27.665261419444565	21.119320459144006
46	20.046406961527133	31.369126090756755	28.708766134990654	19.875700812725455
47	18.990250191953873	27.658645650735703	29.78303176051883	23.568072396791592
48	19.42993321453924	26.66850169740343	33.31889453885194	20.582670549205385
49	20.944751087738613	25.656965148903087	32.92074115925652	20.477542604101775
50	19.16979346246155	31.274573717530824	29.335477421878398	20.220155398129233
51	18.477165939897912	30.35324341682723	28.527919026854224	22.641671616420627
52	18.741120624296773	26.26662996605193	34.08308826014941	20.909161149501887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3968.0
1	4535.5
2	5103.0
3	10368.5
4	15634.0
5	10732.0
6	5830.0
7	5938.5
8	6047.0
9	6081.0
10	6115.0
11	6096.0
12	6077.0
13	6116.5
14	5867.0
15	5578.0
16	5539.5
17	5501.0
18	5481.5
19	5462.0
20	6434.0
21	7406.0
22	9474.0
23	11542.0
24	14219.5
25	16897.0
26	20116.5
27	23336.0
28	26225.0
29	29114.0
30	33982.0
31	38850.0
32	48541.0
33	58232.0
34	66561.0
35	74890.0
36	82158.5
37	89427.0
38	96424.0
39	114863.0
40	126305.0
41	154078.5
42	181852.0
43	181021.5
44	180191.0
45	182377.5
46	184564.0
47	172990.5
48	161417.0
49	159821.0
50	158225.0
51	145729.5
52	133234.0
53	122422.0
54	111610.0
55	97124.0
56	82638.0
57	73778.5
58	64919.0
59	55777.5
60	46636.0
61	40562.5
62	34489.0
63	30594.5
64	22559.5
65	18419.0
66	15593.5
67	12768.0
68	10310.0
69	7852.0
70	6690.0
71	5528.0
72	4795.0
73	4062.0
74	3108.5
75	2155.0
76	1692.0
77	1229.0
78	1017.5
79	806.0
80	557.5
81	309.0
82	252.0
83	195.0
84	135.0
85	75.0
86	49.5
87	24.0
88	16.5
89	7.5
90	6.0
91	6.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2070810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.234017295419687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05851800356346	12.185012794384193
2	9.727995530001259	3.158488953676117
3	3.445329523611805	1.6779461722420237
4	1.7612395215796852	1.1436797141880524
5	1.0736115706195173	0.871451440299997
6	0.7355043671990669	0.7164114370779818
7	0.543296776724585	0.6173922488924877
8	0.4519062496795907	0.586900309856538
9	0.36641688140533807	0.5353576191061202
>10	4.237435589081685	16.338702916461614
>50	1.1171325710639872	12.717212838484116
>100	1.4678253885409227	47.38087186008582
>500	0.01228932834991326	1.2008751378771938
>1k	0.0011989588634061716	0.4479134342623108
>5k	2.997397158515429E-4	0.4217831231054547
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8668	0.4185801691125695	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3395	0.1639455092451746	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT	2860	0.1381102080828275	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14269778492473958	0.0
2	0.0	0.0	0.0	0.3485109691376804	0.0
3	0.0	0.0	0.0	0.46450422781423695	0.0
4	0.0	0.0	0.0	0.6073468835866158	0.0
5	0.0	0.0	0.0	0.9442198946306035	0.0
6	0.0	0.0	0.0	1.1994823281711022	0.0
7	0.0	0.0	0.0	1.3679671239756424	0.0
8	0.0	0.0	0.0	1.7340074656776816	0.0
9	0.0	0.0	0.0	1.8673369357884113	0.0
10	0.0	0.0	0.0	2.1677990737923807	0.0
11	0.0	0.0	0.0	2.614677348477166	0.0
12	0.0	0.0	0.0	3.0060217982335415	0.0
13	0.0	0.0	0.0	3.1519067418063464	0.0
14	0.0	0.0	0.0	3.2096619197318925	0.0
15	4.829028254644318E-5	0.0	0.0	3.308898450364833	0.0
16	4.829028254644318E-5	0.0	0.0	3.5078061241736327	0.0
17	4.829028254644318E-5	0.0	0.0	3.7451045726068544	0.0
18	4.829028254644318E-5	0.0	0.0	4.049429933214539	0.0
19	4.829028254644318E-5	0.0	0.0	4.216852342803057	0.0
20	4.829028254644318E-5	0.0	0.0	4.411945084290688	0.0
21	4.829028254644318E-5	0.0	0.0	4.661654135338346	0.0
22	4.829028254644318E-5	0.0	0.0	4.927154108778691	0.0
23	4.829028254644318E-5	0.0	0.0	5.20303649296652	0.0
24	4.829028254644318E-5	0.0	0.0	5.41715560577745	0.0
25	4.829028254644318E-5	0.0	0.0	5.607322738445343	0.0
26	9.658056509288636E-5	0.0	0.0	5.7933851970967885	0.0
27	9.658056509288636E-5	0.0	0.0	6.007504309907717	0.0
28	9.658056509288636E-5	0.0	0.0	6.1981060551185285	0.0
29	9.658056509288636E-5	0.0	0.0	6.431734442078221	0.0
30	9.658056509288636E-5	0.0	0.0	6.679511881824021	0.0
31	9.658056509288636E-5	0.0	0.0	6.907152273747954	0.0
32	1.4487084763932954E-4	0.0	0.0	7.10895736450954	0.0
33	1.4487084763932954E-4	0.0	0.0	7.327036280489277	0.0
34	1.4487084763932954E-4	0.0	0.0	7.57273723808558	0.0
35	1.4487084763932954E-4	0.0	0.0	7.862575513929332	0.0
36	1.4487084763932954E-4	0.0	0.0	8.088718907094325	0.0
37	1.9316113018577271E-4	0.0	0.0	8.332777995084049	0.0
38	1.9316113018577271E-4	0.0	0.0	8.604555705255432	0.0
39	1.9316113018577271E-4	0.0	0.0	9.082822663595405	0.0
40	1.9316113018577271E-4	0.0	0.0	9.358753338065782	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	35	1.02010745E-7	46.000004	14
TCGCATA	20	6.3126325E-4	46.0	35
CGAACTA	25	3.4181117E-5	46.0	33
TACCCGG	20	6.3126325E-4	46.0	17
CGAACCG	25	3.4181117E-5	46.0	20
GTCGACC	20	6.3126325E-4	46.0	40
CGAAACG	20	6.3126325E-4	46.0	26
CGTGAAT	25	3.4181117E-5	46.0	34
ATAGATC	50	1.6370905E-11	46.0	43
TGCGATT	40	5.6152203E-9	46.0	17
TCGCTAA	20	6.3126325E-4	46.0	29
CTCGACC	20	6.3126325E-4	46.0	31
CGGTCTA	425	0.0	43.835293	31
CTAACGG	160	0.0	43.125	2
TTTCGCG	75	0.0	42.93333	1
TTAATCG	535	0.0	42.560745	20
CTAGACG	65	0.0	42.46154	1
TAATCGT	540	0.0	42.166668	21
GGCGATA	645	0.0	42.077522	8
TCACGAC	455	0.0	41.956043	25
>>END_MODULE