FastQCFastQC Report
Thu 26 May 2016
SRR1544720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544720_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1685649
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85430.5068077636566094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39620.23504300124165828No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT18510.10980933753112303No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG750.046.0000041
ACAACGC206.312298E-446.036
CGTATCA301.861923E-646.038
GTCGAAA253.4178393E-546.013
ACGCATT253.4178393E-546.024
CGACGAA206.312298E-446.023
CTATCGG253.4178393E-546.013
ACCGACA301.861923E-646.018
ATGTCGC501.6370905E-1146.039
TACGACC253.4178393E-546.028
CGCTAGC253.4178393E-546.024
TATTCGA551.8189894E-1246.026
TCGCTAG301.861923E-646.01
TCGCTAC206.312298E-446.019
TCGTCTA206.312298E-446.020
GGACGTA206.312298E-446.08
ACTCGTC206.312298E-446.018
TCGTACA501.6370905E-1146.034
CCGGTTA206.312298E-446.030
ACCGTAT301.861923E-646.036
Produced by FastQC (version 0.11.5)