FastQCFastQC Report
Thu 26 May 2016
SRR1544719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544719_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1200022
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47210.39340945416000705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41950.3495769244230522No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT20430.17024687880722186TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT14330.11941447740124764TruSeq Adapter, Index 15 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14180.11816450031749418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA351.0196163E-746.00000410
ACACGTC351.0196163E-746.00000428
GATAATC351.0196163E-746.00000437
GTTTAGC700.046.0000042
CGAGGCA351.0196163E-746.00000422
CGTGTAT700.046.00000413
CAATCTC351.0196163E-746.00000435
TATCGAT351.0196163E-746.00000413
TGATGCG700.046.0000041
CCCATAG351.0196163E-746.00000443
CTTACCG351.0196163E-746.00000446
TGATCCG301.8614719E-646.035
CTTCGAA253.417246E-546.046
CGGGTGG800.046.06
CTATGCG405.6115823E-946.01
CTCACGA551.8189894E-1246.024
GTACCAT301.8614719E-646.012
TCGCAAA253.417246E-546.023
ACCGGGA405.6115823E-946.04
GTCGATC253.417246E-546.09
Produced by FastQC (version 0.11.5)