##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544719_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1200022
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69990633505052	33.0	31.0	34.0	31.0	34.0
2	33.10581222677584	34.0	33.0	34.0	31.0	34.0
3	33.13790080515191	34.0	33.0	34.0	31.0	34.0
4	36.45650829734788	37.0	37.0	37.0	35.0	37.0
5	36.45995240087265	37.0	37.0	37.0	35.0	37.0
6	36.53144859010918	37.0	37.0	37.0	35.0	37.0
7	36.63823413237424	37.0	37.0	37.0	35.0	37.0
8	36.63972243842196	37.0	37.0	37.0	35.0	37.0
9	38.38197466379783	39.0	39.0	39.0	37.0	39.0
10	38.26696010573139	39.0	39.0	39.0	37.0	39.0
11	38.02142377389748	39.0	39.0	39.0	35.0	39.0
12	37.05554564832978	39.0	35.0	39.0	35.0	39.0
13	36.91627653493019	39.0	35.0	39.0	35.0	39.0
14	37.935391184494954	40.0	35.0	41.0	35.0	41.0
15	37.97754624498551	40.0	35.0	41.0	35.0	41.0
16	38.076616095371584	40.0	35.0	41.0	35.0	41.0
17	38.04989908518344	40.0	35.0	41.0	35.0	41.0
18	38.12856264301821	39.0	36.0	41.0	35.0	41.0
19	38.11066880440525	40.0	36.0	41.0	35.0	41.0
20	37.925371368191584	40.0	35.0	41.0	35.0	41.0
21	37.841538738456464	40.0	35.0	41.0	35.0	41.0
22	37.77130419275647	39.0	35.0	41.0	35.0	41.0
23	37.714466068122086	39.0	35.0	41.0	35.0	41.0
24	37.66282368156584	39.0	35.0	41.0	35.0	41.0
25	37.679719205147904	39.0	35.0	41.0	35.0	41.0
26	37.623916894856926	39.0	35.0	41.0	35.0	41.0
27	37.61336458831588	39.0	35.0	41.0	35.0	41.0
28	37.64780729019968	39.0	35.0	41.0	35.0	41.0
29	37.63642249892085	39.0	35.0	41.0	35.0	41.0
30	37.60082815148389	39.0	35.0	41.0	35.0	41.0
31	37.443204374586465	39.0	35.0	41.0	35.0	41.0
32	37.330209779487376	39.0	35.0	41.0	35.0	41.0
33	37.191840649588094	39.0	35.0	41.0	34.0	41.0
34	37.080532690234016	39.0	35.0	41.0	34.0	41.0
35	36.963634000043335	38.0	35.0	41.0	34.0	41.0
36	36.868226582512655	38.0	35.0	41.0	34.0	41.0
37	36.81591004164924	38.0	35.0	41.0	34.0	41.0
38	36.71959264080159	38.0	35.0	41.0	33.0	41.0
39	36.76004773245824	38.0	35.0	41.0	34.0	41.0
40	36.61214044409186	38.0	35.0	41.0	33.0	41.0
41	36.62106194719763	38.0	35.0	41.0	33.0	41.0
42	36.60616722026763	38.0	35.0	41.0	33.0	41.0
43	36.54998075035291	38.0	35.0	41.0	33.0	41.0
44	36.47336798825355	38.0	35.0	41.0	33.0	41.0
45	36.403228440811915	37.0	35.0	41.0	33.0	41.0
46	36.37372898163534	37.0	35.0	41.0	33.0	41.0
47	36.31699418843988	37.0	35.0	41.0	33.0	41.0
48	36.287018904653415	37.0	35.0	41.0	33.0	41.0
49	36.31890498674191	37.0	35.0	40.0	33.0	41.0
50	36.163579501042484	37.0	35.0	40.0	33.0	41.0
51	36.109591324159055	36.0	35.0	40.0	33.0	41.0
52	35.85071773684149	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	2.0
14	2.0
15	10.0
16	32.0
17	90.0
18	182.0
19	335.0
20	574.0
21	984.0
22	1486.0
23	2432.0
24	4106.0
25	6509.0
26	8863.0
27	9784.0
28	9532.0
29	9676.0
30	10708.0
31	12929.0
32	16596.0
33	23481.0
34	64918.0
35	330629.0
36	43632.0
37	52064.0
38	101190.0
39	487213.0
40	2059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.378906386716245	25.355868475744614	29.08971668852738	13.175508449011769
2	29.586207586194252	25.915191554821494	31.363841662902846	13.134759196081406
3	26.88092384972942	24.767962587352564	33.79329712288608	14.557816440031932
4	24.090891666986106	29.2427138835788	32.52273708315347	14.143657366281618
5	22.385589597524046	34.893526952005885	28.44547849956084	14.275404950909234
6	21.637519978800388	41.621153612183775	26.032856064305488	10.708470344710348
7	88.78803888595375	3.9580941016081375	5.4948159283746465	1.759051084063459
8	89.49519258813588	2.8793638783288973	5.41681735834843	2.2086261751867884
9	85.12477271250027	4.484167790257178	7.548111617953671	2.8429478792888796
10	53.05044407519195	26.698177200084665	11.054464001493306	9.196914723230073
11	49.11418290664671	18.640074931959578	20.45579164381986	11.789950517573844
12	44.86026089521692	20.32246075488616	23.644566516280534	11.172711833616383
13	19.010901466806445	47.02797115386218	22.01276309934318	11.948364279988201
14	12.79076550263245	48.09828486477748	26.6260118564493	12.484937776140772
15	11.16162870347377	23.053744014693063	53.99759337745474	11.78703390437842
16	13.86057922271425	19.336645494832595	49.43001044980842	17.372764832644734
17	13.26909006668211	18.505160738719788	29.862202526287017	38.36354666831108
18	18.097501545804995	22.195259753571182	40.542590052515706	19.16464864810812
19	29.691288993035126	23.077910238312295	26.400099331512255	20.83070143714032
20	31.4076741926398	22.133677549244933	27.66724276721593	18.791405490899333
21	18.07933521218778	29.657539611773785	32.31215761044381	19.95096756559463
22	20.044132524237053	23.447070137047486	25.204454584999276	31.30434275371618
23	13.91691152328874	32.284658114601235	26.35276686594079	27.44566349616924
24	14.362486687744058	22.788332213909413	47.55246153820513	15.296719560141398
25	12.812765099306514	26.270018382996312	41.29990950165914	19.617307016038037
26	13.619666972772166	37.39423110576306	28.71664019492976	20.269461726535013
27	13.093926611345458	41.04899743504703	28.889720355126826	16.967355598480694
28	11.56795458749923	30.924683047477465	41.04091424990542	16.466448115117892
29	12.132694233939043	24.537966803941927	39.85785260603556	23.47148635608347
30	15.657962937346149	36.46599812336774	31.62292024646215	16.253118692823964
31	29.150048915769876	30.81360175063457	24.949459259913567	15.086890073681984
32	31.32017579677706	24.844377853072693	28.59889235364018	15.236553996510063
33	29.722955079156883	28.458811588454214	23.559234747362964	18.25899858502594
34	18.14091741651403	27.91007164868644	26.495347585294272	27.45366334950526
35	17.67025937857806	26.087855055990637	33.846879473876314	22.39500609155499
36	32.629818453328355	24.737463146508983	25.984356953455855	16.64836144670681
37	17.1204361253377	38.332463904828415	29.18979818703324	15.357301782800647
38	17.481929497959204	37.69380894683598	24.865710795302086	19.958550759902735
39	19.457226617512013	36.129587624226886	26.836341333742215	17.576844424518885
40	24.80921183111643	24.723463403170943	26.681927497995872	23.78539726771676
41	15.793043794197107	24.99837502979112	27.330832268075085	31.877748907936688
42	19.66663944494351	23.233157392114478	26.417182351656887	30.68302081128513
43	19.27381331342259	23.336405499232516	29.331128929302963	28.05865225804194
44	15.568131250927067	29.662706183719965	32.79498209199498	21.974180473357986
45	13.832246408815838	44.13369088233382	23.263240173930143	18.770822534920192
46	19.768470911366627	35.986506914039914	26.286851407724193	17.958170766869273
47	18.130334277204916	29.268046752476206	26.85184104958076	25.749777920738122
48	19.192564802978612	25.38011803116943	35.37893471953014	20.048382446321817
49	21.533688549043266	23.752647868122416	36.504580749352925	18.209082833481386
50	18.355413484086124	37.473146325650696	26.804258588592546	17.367181601670637
51	16.564779645706494	35.885342102061465	25.632613402087628	21.917264850144413
52	17.86517247183802	27.163918661491206	37.12273608317181	17.84817278349897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4677.0
1	4315.5
2	3954.0
3	6040.0
4	8126.0
5	5571.5
6	3017.0
7	3165.5
8	3314.0
9	3603.5
10	3893.0
11	3800.5
12	3708.0
13	4072.5
14	4239.5
15	4042.0
16	3952.0
17	3862.0
18	3402.0
19	2942.0
20	3638.0
21	4334.0
22	4739.5
23	5145.0
24	7091.0
25	9037.0
26	9742.5
27	10448.0
28	13244.0
29	16040.0
30	16126.0
31	16212.0
32	21763.0
33	27314.0
34	30878.5
35	34443.0
36	41146.5
37	47850.0
38	49535.5
39	71627.0
40	92033.0
41	118590.5
42	145148.0
43	157180.0
44	169212.0
45	153278.5
46	137345.0
47	125738.0
48	114131.0
49	94494.0
50	74857.0
51	65838.5
52	56820.0
53	51288.5
54	45757.0
55	37306.5
56	28856.0
57	24271.5
58	19687.0
59	17143.0
60	14599.0
61	12962.0
62	11325.0
63	9500.0
64	6465.5
65	5256.0
66	4220.0
67	3184.0
68	2677.0
69	2170.0
70	1800.0
71	1430.0
72	1117.5
73	805.0
74	729.5
75	654.0
76	521.5
77	389.0
78	391.0
79	393.0
80	295.0
81	197.0
82	122.5
83	48.0
84	29.5
85	11.0
86	15.0
87	19.0
88	10.5
89	2.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1200022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.162501455359658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79980507530645	11.34152153314953
2	10.53085532833558	3.193482184841405
3	3.766651646618386	1.7133558322105238
4	1.8815358741090984	1.1411516171796245
5	1.1045624429581204	0.8373964824444061
6	0.7154948376100179	0.6509214909938532
7	0.5375639685564606	0.5705570118910374
8	0.4193304248953176	0.508647854220147
9	0.3323862498750436	0.45358262977247
>10	3.3286316791503676	11.67878914484623
>50	0.9885162445892675	10.603209935777596
>100	1.5626181887638018	53.31967117405818
>500	0.02928527722930746	2.8307007332557697
>1k	0.002762762002764855	1.1570123753592263
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4721	0.39340945416000705	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4195	0.3495769244230522	No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	2043	0.17024687880722186	TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	1433	0.11941447740124764	TruSeq Adapter, Index 15 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1418	0.11816450031749418	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17783007311532623	0.0
2	0.0	0.0	0.0	0.47765790960499055	0.0
3	0.0	0.0	0.0	0.6430715436883657	0.0
4	0.0	0.0	0.0	0.8776505764060992	0.0
5	0.0	0.0	0.0	1.4732229909118333	0.0
6	0.0	0.0	0.0	1.9006318217499345	0.0
7	0.0	0.0	0.0	2.1943764364319986	0.0
8	0.0	0.0	0.0	2.859947567627927	0.0
9	0.0	0.0	0.0	3.0721103446436815	0.0
10	0.0	0.0	0.0	3.4877693908945004	0.0
11	0.0	0.0	0.0	3.991926814675064	0.0
12	8.33318055835643E-5	0.0	0.0	4.426252185376601	0.0
13	8.33318055835643E-5	0.0	0.0	4.581416007373198	0.0
14	8.33318055835643E-5	0.0	0.0	4.632581736001507	0.0
15	8.33318055835643E-5	0.0	0.0	4.726580012699767	0.0
16	8.33318055835643E-5	0.0	0.0	4.942492720966782	0.0
17	8.33318055835643E-5	0.0	0.0	5.219654306337717	0.0
18	8.33318055835643E-5	0.0	0.0	5.638729956617462	0.0
19	8.33318055835643E-5	0.0	0.0	5.811310125981024	0.0
20	1.666636111671286E-4	0.0	0.0	6.0206396216069376	0.0
21	1.666636111671286E-4	0.0	0.0	6.262135194188106	0.0
22	2.499954167506929E-4	0.0	0.0	6.495380918016503	0.0
23	2.499954167506929E-4	0.0	0.0	6.822874913959911	0.0
24	3.333272223342572E-4	0.0	0.0	7.014538066802109	0.0
25	3.333272223342572E-4	0.0	0.0	7.178951719218481	0.0
26	3.333272223342572E-4	0.0	0.0	7.361281709835319	0.0
27	3.333272223342572E-4	0.0	0.0	7.521695435583681	0.0
28	3.333272223342572E-4	0.0	0.0	7.7362748349613595	0.0
29	3.333272223342572E-4	0.0	0.0	7.925771360858384	0.0
30	3.333272223342572E-4	0.0	0.0	8.173683482469489	0.0
31	3.333272223342572E-4	0.0	0.0	8.398012703100443	0.0
32	3.333272223342572E-4	0.0	0.0	8.585425933857879	0.0
33	3.333272223342572E-4	0.0	0.0	8.827504829078133	0.0
34	3.333272223342572E-4	0.0	0.0	9.037667642759883	0.0
35	3.333272223342572E-4	0.0	0.0	9.307996020072965	0.0
36	3.333272223342572E-4	0.0	0.0	9.511575621113613	0.0
37	3.333272223342572E-4	0.0	0.0	9.716655194654765	0.0
38	3.333272223342572E-4	0.0	0.0	9.913901578471062	0.0
39	3.333272223342572E-4	0.0	0.0	10.117147852289374	0.0
40	3.333272223342572E-4	0.0	0.0	10.352310207646193	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	35	1.0196163E-7	46.000004	10
ACACGTC	35	1.0196163E-7	46.000004	28
GATAATC	35	1.0196163E-7	46.000004	37
GTTTAGC	70	0.0	46.000004	2
CGAGGCA	35	1.0196163E-7	46.000004	22
CGTGTAT	70	0.0	46.000004	13
CAATCTC	35	1.0196163E-7	46.000004	35
TATCGAT	35	1.0196163E-7	46.000004	13
TGATGCG	70	0.0	46.000004	1
CCCATAG	35	1.0196163E-7	46.000004	43
CTTACCG	35	1.0196163E-7	46.000004	46
TGATCCG	30	1.8614719E-6	46.0	35
CTTCGAA	25	3.417246E-5	46.0	46
CGGGTGG	80	0.0	46.0	6
CTATGCG	40	5.6115823E-9	46.0	1
CTCACGA	55	1.8189894E-12	46.0	24
GTACCAT	30	1.8614719E-6	46.0	12
TCGCAAA	25	3.417246E-5	46.0	23
ACCGGGA	40	5.6115823E-9	46.0	4
GTCGATC	25	3.417246E-5	46.0	9
>>END_MODULE