FastQCFastQC Report
Thu 26 May 2016
SRR1544718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544718_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1164387
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76160.6540780685459388No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53890.4628186333238004No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT33710.2895085568629674Illumina PCR Primer Index 2 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT25710.22080287739385615Illumina PCR Primer Index 2 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22360.19203237411616583No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT16270.13973017562030493Illumina PCR Primer Index 2 (96% over 25bp)
AAAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT14180.12178081685899962Illumina PCR Primer Index 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG501.6370905E-1146.033
CGGGTAT1000.046.06
CGTGGGA900.046.04
AACCGGT206.311489E-446.023
AACCGGA206.311489E-446.027
CGGAACC453.092282E-1046.015
AACCGAT301.8614246E-646.042
TCGTTGC206.311489E-446.015
GTACCGG405.6115823E-946.02
ACCGGTT206.311489E-446.024
TACCCGC206.311489E-446.027
ACGCCTA206.311489E-446.030
ACGCCGA206.311489E-446.012
GTCGAAT405.6115823E-946.012
CACGGTC206.311489E-446.037
CAAGTCG405.6115823E-946.01
CTCCGCA253.4171833E-546.011
TTTACGT405.6115823E-946.025
GTTTCGA501.6370905E-1146.034
CGAAACA453.092282E-1046.033
Produced by FastQC (version 0.11.5)