##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544718_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1164387
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64485862518218	33.0	31.0	34.0	31.0	34.0
2	33.04800465824507	34.0	33.0	34.0	31.0	34.0
3	33.07269833826726	34.0	33.0	34.0	31.0	34.0
4	36.40411306550142	37.0	37.0	37.0	35.0	37.0
5	36.40696778648336	37.0	37.0	37.0	35.0	37.0
6	36.47043122260898	37.0	37.0	37.0	35.0	37.0
7	36.60262438519152	37.0	37.0	37.0	35.0	37.0
8	36.604148792454744	37.0	37.0	37.0	35.0	37.0
9	38.319035681435814	39.0	39.0	39.0	37.0	39.0
10	38.19866075454295	39.0	39.0	39.0	37.0	39.0
11	37.96008200022845	39.0	38.0	39.0	35.0	39.0
12	37.09244606818867	39.0	35.0	39.0	35.0	39.0
13	36.93841909949184	39.0	35.0	39.0	35.0	39.0
14	37.99280565653859	40.0	35.0	41.0	35.0	41.0
15	38.05787165263783	40.0	35.0	41.0	35.0	41.0
16	38.14125715934651	40.0	35.0	41.0	35.0	41.0
17	38.09587018748921	40.0	35.0	41.0	35.0	41.0
18	38.125841322515626	39.0	36.0	41.0	35.0	41.0
19	38.08625396882652	40.0	36.0	41.0	35.0	41.0
20	37.92866289300722	40.0	35.0	41.0	35.0	41.0
21	37.83309758697065	40.0	35.0	41.0	35.0	41.0
22	37.765091846611135	39.0	35.0	41.0	35.0	41.0
23	37.69969091032449	39.0	35.0	41.0	35.0	41.0
24	37.64048636750496	39.0	35.0	41.0	35.0	41.0
25	37.64976592833826	39.0	35.0	41.0	35.0	41.0
26	37.59641940351447	39.0	35.0	41.0	35.0	41.0
27	37.57964061776712	39.0	35.0	41.0	35.0	41.0
28	37.58379129962805	39.0	35.0	41.0	35.0	41.0
29	37.56378334694565	39.0	35.0	41.0	35.0	41.0
30	37.515287443092376	39.0	35.0	41.0	35.0	41.0
31	37.37568523180008	39.0	35.0	41.0	34.0	41.0
32	37.22937476972862	39.0	35.0	41.0	34.0	41.0
33	37.078947978635966	39.0	35.0	41.0	34.0	41.0
34	36.93596630673479	38.0	35.0	41.0	34.0	41.0
35	36.802898864381	38.0	35.0	41.0	34.0	41.0
36	36.73004507951394	38.0	35.0	41.0	33.0	41.0
37	36.68385425120686	38.0	35.0	41.0	33.0	41.0
38	36.57545128896149	38.0	35.0	41.0	33.0	41.0
39	36.58107742528902	38.0	35.0	41.0	33.0	41.0
40	36.42471703995321	38.0	35.0	41.0	32.0	41.0
41	36.44919859119004	38.0	35.0	41.0	33.0	41.0
42	36.4624433285497	38.0	35.0	41.0	33.0	41.0
43	36.406277294404696	38.0	35.0	41.0	33.0	41.0
44	36.31910524593627	38.0	35.0	41.0	33.0	41.0
45	36.25277420651381	37.0	35.0	41.0	33.0	41.0
46	36.20143388753052	37.0	35.0	41.0	33.0	41.0
47	36.13869959042827	37.0	35.0	41.0	33.0	41.0
48	36.09410187506388	37.0	35.0	40.0	33.0	41.0
49	36.105120548408735	37.0	35.0	40.0	32.0	41.0
50	35.98148811348804	37.0	35.0	40.0	32.0	41.0
51	35.893919289720685	36.0	35.0	40.0	32.0	41.0
52	35.62131318882811	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	0.0
14	9.0
15	13.0
16	26.0
17	77.0
18	188.0
19	350.0
20	552.0
21	1016.0
22	1632.0
23	2778.0
24	4661.0
25	7356.0
26	9782.0
27	10702.0
28	10507.0
29	10730.0
30	11883.0
31	13693.0
32	17866.0
33	25117.0
34	64750.0
35	298761.0
36	47068.0
37	55653.0
38	103297.0
39	464112.0
40	1804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.675637051942353	25.165945686442736	29.29876407070845	13.85965319090646
2	30.42115722693572	25.657534823044227	29.891178791930862	14.030129158089192
3	27.88557412612817	27.304839370415507	31.512976355799232	13.296610147657093
4	24.47004303551998	28.836546612080006	32.79390786740147	13.899502484998544
5	22.907332355995045	32.84655359429468	29.8230742871571	14.423039762553172
6	20.63162848777941	41.47461282202567	27.046162487214303	10.847596202980624
7	86.8289494815727	4.819875178956824	6.393063474600799	1.9581118648696696
8	87.32775271451845	3.3719029841453056	6.681197917874384	2.619146383461856
9	83.25324827570215	5.42860749905315	8.094559626653338	3.223584598591362
10	52.97972237752568	25.27201007912318	12.619945086985684	9.128322456365453
11	44.81903353438333	19.97986923591555	22.783662132950646	12.417435096750479
12	41.99325481991812	20.854320771358665	24.423752583977667	12.728671824745552
13	20.0271902726499	43.28363336244737	24.226739048099986	12.462437316802747
14	12.65429792672024	45.70473562483951	27.807507297831393	13.833459150608862
15	10.90144427926454	25.39705441575696	50.55999422872293	13.141507076255573
16	13.70695481828636	20.072536021099513	49.60567234089697	16.614836819717155
17	13.906544817144129	19.753140493667484	30.057103007848767	36.28321168133962
18	16.7987962764957	23.790372101371794	39.09456220311632	20.316269419016187
19	26.504761732997707	24.28015771388722	29.27428767239758	19.940792880717492
20	28.92010989473431	23.13457639083913	27.97334563164996	19.971968082776602
21	19.18503040655727	29.793015552389367	31.943846848169894	19.078107192883465
22	19.51679295629374	23.49553885434997	26.211474363763937	30.77619382559235
23	15.349535850194137	31.049642429879416	27.057670688525377	26.543151031401074
24	15.45482730398055	22.89281828120719	44.1427978842086	17.509556530603657
25	13.684539590359563	24.5241487581019	39.69960159294118	22.091710058597354
26	12.37509522177764	36.228418901963	29.512696380155397	21.883789496103958
27	13.93789178340191	39.68276870147125	28.087998234264038	18.291341280862806
28	12.389952824962833	31.312355771749427	38.50532511956935	17.792366283718387
29	13.10827070381239	25.305418215765034	36.944074435733135	24.64223664468944
30	15.98162810130996	34.07887583767253	31.307889902583934	18.631606158433577
31	26.789203245999825	29.665051224378146	27.19954791662909	16.34619761299293
32	28.932391034939414	25.42178846036584	29.098315250857322	16.54750525383743
33	27.984853832961033	27.59314557788776	25.01058496874321	19.41141562040799
34	19.29684889989325	28.021611371477007	26.612629649764212	26.06891007886553
35	17.23696674730996	28.012593751046687	31.732147473305695	23.018292028337658
36	30.106313450768518	24.96772980117435	26.39071030507898	18.535246442978153
37	17.54201996415281	36.10028280975312	29.489422331235232	16.86827489485884
38	18.41793149528464	35.12345981190103	25.461809518656597	20.996799174157733
39	19.763532227687186	33.353000334081365	28.191572046063722	18.691895392167723
40	24.041577241930735	25.39894382194236	28.89357232603937	21.66590661008754
41	14.621169765722222	26.12748167061295	29.43548837285198	29.81586019081285
42	19.8744919000298	23.08459300902535	27.284571194972116	29.75634389597273
43	20.481850106536744	22.76210572601721	29.423207232646874	27.33283693479917
44	16.532561768552895	29.72190517413884	31.21805722667807	22.527475830630195
45	15.148399973548313	40.02861591549888	24.384418582481597	20.4385655284712
46	20.289474204023232	34.91897453338109	26.369239780244886	18.4223114823508
47	19.551403442326308	29.237100723384923	26.31109759899415	24.900398235294624
48	20.436676122285803	26.027686671184064	33.4054742967759	20.130162909754233
49	22.18841330245013	24.144034586439044	34.027690106468036	19.639862004642787
50	19.6842630499997	34.547019161155184	27.451440113982724	18.317277674862396
51	17.52132237821274	33.14645388517735	25.825777855644215	23.506445880965693
52	18.1730816300766	26.710621125107032	36.06738996570728	19.04890727910909
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8421.0
1	6689.5
2	4958.0
3	7270.0
4	9582.0
5	6482.0
6	3382.0
7	3607.0
8	3832.0
9	4175.5
10	4519.0
11	4813.0
12	5107.0
13	4955.5
14	4498.5
15	4193.0
16	4204.5
17	4216.0
18	3868.5
19	3521.0
20	4560.5
21	5600.0
22	5455.0
23	5310.0
24	6579.5
25	7849.0
26	8243.5
27	8638.0
28	10563.0
29	12488.0
30	15796.5
31	19105.0
32	21986.0
33	24867.0
34	27900.5
35	30934.0
36	34238.5
37	37543.0
38	39277.5
39	58164.0
40	75316.0
41	95877.5
42	116439.0
43	132342.5
44	148246.0
45	141940.0
46	135634.0
47	127614.0
48	119594.0
49	105896.5
50	92199.0
51	81053.0
52	69907.0
53	60161.5
54	50416.0
55	41274.5
56	32133.0
57	27474.5
58	22816.0
59	19761.5
60	16707.0
61	14215.5
62	11724.0
63	9681.0
64	6770.0
65	5902.0
66	4881.0
67	3860.0
68	3046.0
69	2232.0
70	1813.5
71	1395.0
72	1113.5
73	832.0
74	784.5
75	737.0
76	556.0
77	375.0
78	288.5
79	202.0
80	163.0
81	124.0
82	83.5
83	43.0
84	28.0
85	13.0
86	16.0
87	19.0
88	10.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1164387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.03531810063957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61367264593443	12.540323300798429
2	11.021753654529428	3.7551815906359103
3	4.243720309807704	2.1687937622315685
4	2.085235853041874	1.4209062428570733
5	1.1875773324988934	1.011537881411383
6	0.7722707450038362	0.7893526680574956
7	0.5867983047504234	0.6997407047637652
8	0.40566877510353927	0.5528557301908484
9	0.3318719508879934	0.5088189826851122
>10	3.4558862519102282	13.73600266157437
>50	0.9741127030542313	11.746432417260518
>100	1.2981418766268742	46.774074467901514
>500	0.019239232180897507	2.1159482678319717
>1k	0.0030377735022469755	1.0583622547004492
>5k	0.0010125911674156583	1.1216690670996126
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7616	0.6540780685459388	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5389	0.4628186333238004	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	3371	0.2895085568629674	Illumina PCR Primer Index 2 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2571	0.22080287739385615	Illumina PCR Primer Index 2 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2236	0.19203237411616583	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	1627	0.13973017562030493	Illumina PCR Primer Index 2 (96% over 25bp)
AAAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	1418	0.12178081685899962	Illumina PCR Primer Index 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30290616435944406	0.0
2	0.0	0.0	0.0	0.8595939322579177	0.0
3	0.0	0.0	0.0	1.1490166070215486	0.0
4	0.0	0.0	0.0	1.5064579044595998	0.0
5	0.0	0.0	0.0	2.4742632818813677	0.0
6	0.0	0.0	0.0	3.1310036955067346	0.0
7	8.588209933638902E-5	0.0	0.0	3.580167075036049	0.0
8	8.588209933638902E-5	0.0	0.0	4.494210258273237	0.0
9	8.588209933638902E-5	0.0	0.0	4.7796823564673945	0.0
10	8.588209933638902E-5	0.0	0.0	5.2821785196846065	0.0
11	8.588209933638902E-5	0.0	0.0	5.975676471825948	0.0
12	8.588209933638902E-5	0.0	0.0	6.563711205982203	0.0
13	1.7176419867277805E-4	0.0	0.0	6.775754109243748	0.0
14	1.7176419867277805E-4	0.0	0.0	6.848839775779015	0.0
15	1.7176419867277805E-4	0.0	0.0	6.978264099478953	0.0
16	1.7176419867277805E-4	0.0	0.0	7.278078508262287	0.0
17	1.7176419867277805E-4	0.0	0.0	7.624441014885944	0.0
18	1.7176419867277805E-4	0.0	0.0	8.162148838831076	0.0
19	1.7176419867277805E-4	0.0	0.0	8.397809319410127	0.0
20	1.7176419867277805E-4	0.0	0.0	8.637162730260643	0.0
21	2.5764629800916703E-4	0.0	0.0	8.98326759058629	0.0
22	6.011746953547231E-4	0.0	0.0	9.331519503395349	0.0
23	6.011746953547231E-4	0.0	0.0	9.747188864183471	0.0
24	7.729388940275012E-4	0.0	0.0	10.03497977905971	0.0
25	7.729388940275012E-4	0.0	0.0	10.262567342301143	0.0
26	7.729388940275012E-4	0.0	0.0	10.51686423843619	0.0
27	7.729388940275012E-4	0.0	0.0	10.736550648538673	0.0
28	7.729388940275012E-4	0.0	0.0	10.966027617965505	0.0
29	7.729388940275012E-4	0.0	0.0	11.222986859179981	0.0
30	7.729388940275012E-4	0.0	0.0	11.544185910698076	0.0
31	7.729388940275012E-4	0.0	0.0	11.881530796891411	0.0
32	7.729388940275012E-4	0.0	0.0	12.146820601741517	0.0
33	7.729388940275012E-4	0.0	0.0	12.418465681942516	0.0
34	7.729388940275012E-4	0.0	0.0	12.664088486044589	0.0
35	7.729388940275012E-4	0.0	0.0	13.022388604476003	0.0
36	7.729388940275012E-4	0.0	0.0	13.300474842127231	0.0
37	7.729388940275012E-4	0.0	0.0	13.580450485963858	0.0
38	8.588209933638902E-4	0.0	0.0	13.8429920636352	0.0
39	8.588209933638902E-4	0.0	0.0	14.095914846180866	0.0
40	8.588209933638902E-4	0.0	0.0	14.379411656090285	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	50	1.6370905E-11	46.0	33
CGGGTAT	100	0.0	46.0	6
CGTGGGA	90	0.0	46.0	4
AACCGGT	20	6.311489E-4	46.0	23
AACCGGA	20	6.311489E-4	46.0	27
CGGAACC	45	3.092282E-10	46.0	15
AACCGAT	30	1.8614246E-6	46.0	42
TCGTTGC	20	6.311489E-4	46.0	15
GTACCGG	40	5.6115823E-9	46.0	2
ACCGGTT	20	6.311489E-4	46.0	24
TACCCGC	20	6.311489E-4	46.0	27
ACGCCTA	20	6.311489E-4	46.0	30
ACGCCGA	20	6.311489E-4	46.0	12
GTCGAAT	40	5.6115823E-9	46.0	12
CACGGTC	20	6.311489E-4	46.0	37
CAAGTCG	40	5.6115823E-9	46.0	1
CTCCGCA	25	3.4171833E-5	46.0	11
TTTACGT	40	5.6115823E-9	46.0	25
GTTTCGA	50	1.6370905E-11	46.0	34
CGAAACA	45	3.092282E-10	46.0	33
>>END_MODULE