FastQCFastQC Report
Thu 26 May 2016
SRR1544717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544717_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1131320
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88830.7851889827811759No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51830.4581373970229467No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT38600.3411943570342609Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT35150.31069900647031784Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25570.22601916345507903No Hit
GGAGAAGGGAAGGAGAAGAGAAGGAGAAGAGAAGGAGAAGAGAAGGAGAAGA21860.19322561255878087No Hit
AAAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT17440.15415620690874376No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT16000.14142771276031538Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT13250.11711982462963619Illumina Single End Adapter 1 (95% over 21bp)
CATGTTATTCAATTCCAGTCACCTCTTCTGCAATCATGACCTCTCGATGTCT12340.10907612346639324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA351.01954356E-746.00000430
TCGCCGG206.311411E-446.00000414
CGGGTTG206.311411E-446.0000046
GTGCAAC206.311411E-446.00000442
CGGGTCA351.01954356E-746.0000046
CGGAACC351.01954356E-746.00000439
GATACCC206.311411E-446.00000439
CGAACGG206.311411E-446.0000042
TCCGCTA206.311411E-446.00000443
CGTATGG750.046.0000042
GATCGGA351.01954356E-746.0000048
CTCAACT206.311411E-446.00000412
GTCGAAT405.6115823E-946.00000446
TAAAGCG351.01954356E-746.0000041
ACGTTGT206.311411E-446.00000443
ACGCATC206.311411E-446.00000423
GCGGTAA206.311411E-446.00000446
GACACGG405.6115823E-946.0000042
TTTCGCG351.01954356E-746.0000041
CGTTGTA206.311411E-446.00000444
Produced by FastQC (version 0.11.5)