##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544717_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1131320
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.661737616235904	33.0	31.0	34.0	31.0	34.0
2	33.043867340805434	34.0	33.0	34.0	31.0	34.0
3	33.06371230067532	34.0	33.0	34.0	31.0	34.0
4	36.403349185022805	37.0	37.0	37.0	35.0	37.0
5	36.406496835554925	37.0	37.0	37.0	35.0	37.0
6	36.46994926280805	37.0	37.0	37.0	35.0	37.0
7	36.59695134886681	37.0	37.0	37.0	35.0	37.0
8	36.590121274263694	37.0	37.0	37.0	35.0	37.0
9	38.33000919280133	39.0	39.0	39.0	37.0	39.0
10	38.202320298412474	39.0	39.0	39.0	37.0	39.0
11	37.95161669554149	39.0	38.0	39.0	35.0	39.0
12	36.88925679736944	39.0	35.0	39.0	35.0	39.0
13	36.70943499628752	39.0	35.0	39.0	35.0	39.0
14	37.672605452038326	40.0	35.0	41.0	35.0	41.0
15	37.75728706289998	40.0	35.0	41.0	35.0	41.0
16	37.87340540253863	40.0	35.0	41.0	35.0	41.0
17	37.84439592688187	39.0	35.0	41.0	35.0	41.0
18	37.90160343669342	39.0	36.0	41.0	35.0	41.0
19	37.81991920941908	38.0	36.0	41.0	35.0	41.0
20	37.62943375879504	38.0	35.0	41.0	35.0	41.0
21	37.52030018032033	38.0	35.0	41.0	35.0	41.0
22	37.458083477707454	38.0	35.0	41.0	35.0	41.0
23	37.40708110879327	38.0	35.0	41.0	35.0	41.0
24	37.35925821164657	38.0	35.0	41.0	35.0	41.0
25	37.35121097479051	38.0	35.0	41.0	35.0	41.0
26	37.287279461160416	38.0	35.0	41.0	35.0	41.0
27	37.25112523423965	38.0	35.0	41.0	34.0	41.0
28	37.24387352826786	37.0	35.0	41.0	34.0	41.0
29	37.24548492027013	37.0	35.0	41.0	35.0	41.0
30	37.20136831312096	37.0	35.0	41.0	34.0	41.0
31	37.04416787469505	37.0	35.0	41.0	34.0	41.0
32	36.88367393840823	37.0	35.0	41.0	34.0	41.0
33	36.72953629388679	37.0	35.0	41.0	34.0	41.0
34	36.5934907895202	37.0	35.0	41.0	34.0	41.0
35	36.4792976346215	37.0	35.0	41.0	33.0	41.0
36	36.36365749743662	37.0	35.0	41.0	33.0	41.0
37	36.33579623802284	37.0	35.0	41.0	33.0	41.0
38	36.22867535268536	36.0	35.0	41.0	33.0	41.0
39	36.24490595057102	36.0	35.0	41.0	33.0	41.0
40	36.13396121344977	36.0	35.0	41.0	32.0	41.0
41	36.12134674539476	36.0	35.0	41.0	33.0	41.0
42	36.11520613089135	36.0	35.0	41.0	33.0	41.0
43	36.026324116960716	36.0	35.0	41.0	33.0	41.0
44	35.92641958066683	35.0	35.0	41.0	33.0	41.0
45	35.85529911961249	35.0	35.0	40.0	32.0	41.0
46	35.830715447441925	35.0	35.0	40.0	32.0	41.0
47	35.771354700703604	35.0	35.0	40.0	32.0	41.0
48	35.73799278718665	35.0	35.0	40.0	32.0	41.0
49	35.75099352968214	36.0	35.0	40.0	32.0	41.0
50	35.60231411095004	35.0	35.0	40.0	32.0	41.0
51	35.54847965208783	35.0	35.0	40.0	32.0	41.0
52	35.34006116748577	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	4.0
13	3.0
14	16.0
15	12.0
16	36.0
17	83.0
18	214.0
19	401.0
20	743.0
21	1171.0
22	1907.0
23	3224.0
24	5196.0
25	7883.0
26	10448.0
27	11213.0
28	10620.0
29	10762.0
30	11713.0
31	13785.0
32	18004.0
33	25357.0
34	66326.0
35	338259.0
36	44652.0
37	50729.0
38	91056.0
39	405649.0
40	1850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.058215182264966	23.971555351271082	29.694781317399144	14.27544814906481
2	28.848955202771982	27.12565852278754	30.110313615953043	13.915072658487432
3	25.175281971502315	27.497701799667645	33.51421348513241	13.812802743697628
4	23.565127461726128	29.415549977017996	32.651504437294484	14.36781812396139
5	21.66115687869038	35.099971714457446	29.705211611215216	13.533659795636954
6	20.125428702754306	41.92200261641268	26.735848389491924	11.216720291341089
7	85.82514231163596	5.142576812926493	6.516105080790581	2.516175794646961
8	86.2298907470919	3.8121839974543015	6.984407594668175	2.973517660785631
9	83.13704345366475	4.986387582646819	8.854612311282397	3.021956652406039
10	55.0017678464095	24.44737121238907	12.147226248983488	8.40363469221794
11	50.28365095640491	17.78639111833964	20.646413039635117	11.283544885620337
12	45.4476187108864	20.348619311954177	21.894159035462998	12.309602941696426
13	19.160714917088004	47.168617190538484	22.31649754269349	11.35417034968002
14	10.83221369727398	49.415461584697525	27.107891666372026	12.644433051656472
15	9.470353215712619	25.02704805006541	53.774705653572816	11.727893080649153
16	11.017660785630945	19.37798324081604	52.35839550259873	17.245960470954284
17	11.925096347629317	19.31336845454867	29.618940706431424	39.14259449139059
18	17.617738570872962	23.413446239790687	39.08363681363363	19.88517837570272
19	27.969716791005194	22.82316232365732	27.35185447088357	21.85526641445391
20	31.1204610543436	22.917830498886257	27.078280239012837	18.883428207757312
21	17.604126153519783	32.82519534702825	30.83557260545204	18.73510589399993
22	19.173001449634057	25.27825902485592	24.186437082346284	31.362302443163735
23	14.402379521267191	33.5231411095004	24.996641091821942	27.07783827741046
24	14.758247003500335	22.890782448820847	45.821960188098856	16.52901035957996
25	12.647173213591204	25.77016228830039	41.07299437824842	20.509670119859987
26	12.252501502669448	37.908372520595414	28.504932291482515	21.334193685252625
27	13.090195523812891	42.70003182123537	27.72575398649365	16.484018668458084
28	11.039405296467843	32.06678923735106	41.25013258848072	15.643672877700384
29	11.351341795424814	24.808630626171198	39.59834529576071	24.241682282643286
30	14.918944242124244	36.026765194639886	30.674256620584806	18.38003394265106
31	29.19023795212672	29.1029947318177	25.419598345295764	16.287168970759822
32	30.681504790863766	25.815153979422266	28.491673443411237	15.011667786302727
33	28.156843333451192	29.29215429763462	24.205795000530355	18.345207368383836
34	17.533765866421525	27.95530884276774	27.700473782837747	26.81045150797299
35	17.897765442138386	25.65578262560549	32.08190432415232	24.36454760810381
36	32.37032846586288	23.21588940352862	27.439539652794963	16.97424247781353
37	16.716048509705477	35.809938832514234	31.207350705370718	16.266661952409574
38	17.275748682954426	36.95523812891136	24.0694940423576	21.699519145776616
39	18.704964112717885	34.60426758123255	29.511897606335964	17.17887069971361
40	25.613442704097867	24.35694586854294	25.956935261464487	24.07267616589471
41	15.206926422232437	23.116624827634976	28.190785984513667	33.485662765618926
42	19.982233143584484	21.021638440052328	27.931442916239433	31.06468550012375
43	19.872449881554292	22.55798536223173	29.409362514584732	28.160202241629246
44	14.58225789343422	30.23901283456493	32.14395573312591	23.03477353887494
45	14.194569175830004	42.59785029876604	23.232241982816532	19.97533854258742
46	21.09137998090726	35.42596259236998	25.466799844429516	18.01585758229325
47	18.16806915815154	28.411943570342608	27.229696283986847	26.190290987519006
48	19.69319025563059	24.592688187250292	35.083795919810484	20.63032563730863
49	22.19080366297776	22.481791181982107	36.10419686737617	19.22320828766397
50	18.92665205246968	35.3830923169395	27.144928048651135	18.54532758193968
51	15.779355089629812	35.29947318176997	24.15523459321854	24.765937135381677
52	16.97822013223491	26.990153095499064	36.95541491355231	19.076211858713716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9841.0
1	7480.0
2	5119.0
3	6982.5
4	8846.0
5	6050.0
6	3254.0
7	3526.5
8	3799.0
9	4251.0
10	4703.0
11	5096.5
12	5490.0
13	5321.0
14	5304.5
15	5457.0
16	5271.5
17	5086.0
18	4824.0
19	4562.0
20	4762.0
21	4962.0
22	5040.0
23	5118.0
24	5105.0
25	5092.0
26	5865.5
27	6639.0
28	8157.5
29	9676.0
30	12820.5
31	15965.0
32	19077.5
33	22190.0
34	24817.0
35	27444.0
36	27761.0
37	28078.0
38	33435.0
39	58124.5
40	77457.0
41	103918.0
42	130379.0
43	142089.0
44	153799.0
45	146713.5
46	139628.0
47	129839.0
48	120050.0
49	105773.0
50	91496.0
51	77933.5
52	64371.0
53	54929.5
54	45488.0
55	37584.0
56	29680.0
57	23233.0
58	16786.0
59	14781.0
60	12776.0
61	11041.0
62	9306.0
63	7247.0
64	4378.0
65	3568.0
66	2852.5
67	2137.0
68	1686.5
69	1236.0
70	1035.0
71	834.0
72	835.0
73	836.0
74	609.0
75	382.0
76	306.5
77	231.0
78	201.0
79	171.0
80	170.0
81	169.0
82	111.0
83	53.0
84	42.0
85	31.0
86	15.5
87	0.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1131320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.867925672651282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2330622217101	12.352898503364525
2	11.20981500679861	3.7817265267769975
3	4.402249244920146	2.227704391673947
4	2.22635246425736	1.5021579155286866
5	1.3150693772363615	1.109124625480138
6	0.8592147789558054	0.8695902616962017
7	0.5718530199055027	0.6752181964813037
8	0.4801571371557697	0.6479403920589241
9	0.3369833109334872	0.5115788497574999
>10	3.137678133622273	11.923456546618153
>50	0.8851252865581413	10.708527965593726
>100	1.319258165203254	48.77456501710134
>500	0.01685952926777321	1.8724105008223124
>1k	0.005268602896179129	1.7930492317903544
>5k	0.0010537205792358257	1.2500510752559042
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8883	0.7851889827811759	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5183	0.4581373970229467	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	3860	0.3411943570342609	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	3515	0.31069900647031784	Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2557	0.22601916345507903	No Hit
GGAGAAGGGAAGGAGAAGAGAAGGAGAAGAGAAGGAGAAGAGAAGGAGAAGA	2186	0.19322561255878087	No Hit
AAAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	1744	0.15415620690874376	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	1600	0.14142771276031538	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	1325	0.11711982462963619	Illumina Single End Adapter 1 (95% over 21bp)
CATGTTATTCAATTCCAGTCACCTCTTCTGCAATCATGACCTCTCGATGTCT	1234	0.10907612346639324	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3576353286426475	0.0
2	0.0	0.0	0.0	1.0560230527171799	0.0
3	0.0	0.0	0.0	1.4127744581550754	0.0
4	0.0	0.0	0.0	1.8423611356645335	0.0
5	0.0	0.0	0.0	2.9510660113849307	0.0
6	0.0	0.0	0.0	3.745271010854577	0.0
7	0.0	0.0	0.0	4.319202347700032	0.0
8	0.0	0.0	0.0	5.49101934023972	0.0
9	0.0	0.0	0.0	5.8678358024254855	0.0
10	0.0	0.0	0.0	6.521143443057667	0.0
11	0.0	0.0	0.0	7.413463918254782	0.0
12	0.0	0.0	0.0	8.150037124774599	0.0
13	0.0	0.0	0.0	8.416716755648268	0.0
14	0.0	0.0	0.0	8.510059046070078	0.0
15	0.0	0.0	0.0	8.669695576848284	0.0
16	0.0	0.0	0.0	9.030513029028038	0.0
17	0.0	0.0	0.0	9.449492628080472	0.0
18	0.0	0.0	0.0	10.098380652688894	0.0
19	0.0	0.0	0.0	10.361524590743556	0.0
20	0.0	0.0	0.0	10.646766608917018	0.0
21	0.0	0.0	0.0	11.026058056076089	0.0
22	0.0	0.0	0.0	11.38784782378107	0.0
23	0.0	0.0	0.0	11.78278471166425	0.0
24	1.7678464095039422E-4	0.0	0.0	12.0623696213273	0.0
25	1.7678464095039422E-4	0.0	0.0	12.297316409150373	0.0
26	1.7678464095039422E-4	0.0	0.0	12.543400629353322	0.0
27	1.7678464095039422E-4	0.0	0.0	12.760050206838029	0.0
28	3.5356928190078844E-4	0.0	0.0	12.97723013824559	0.0
29	3.5356928190078844E-4	0.0	0.0	13.221458119718559	0.0
30	3.5356928190078844E-4	0.0	0.0	13.558056076088109	0.0
31	3.5356928190078844E-4	0.0	0.0	13.880511261181628	0.0
32	4.4196160237598556E-4	0.0	0.0	14.137644521443978	0.0
33	4.4196160237598556E-4	0.0	0.0	14.366934200756639	0.0
34	5.303539228511827E-4	0.0	0.0	14.6297245695294	0.0
35	5.303539228511827E-4	0.0	0.0	14.980642081815931	0.0
36	5.303539228511827E-4	0.0	0.0	15.279231340381148	0.0
37	5.303539228511827E-4	0.0	0.0	15.581886645688222	0.0
38	5.303539228511827E-4	0.0	0.0	15.846621645511439	0.0
39	6.187462433263798E-4	0.0	0.0	16.095622812290067	0.0
40	7.071385638015769E-4	0.0	0.0	16.392179047484355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	35	1.01954356E-7	46.000004	30
TCGCCGG	20	6.311411E-4	46.000004	14
CGGGTTG	20	6.311411E-4	46.000004	6
GTGCAAC	20	6.311411E-4	46.000004	42
CGGGTCA	35	1.01954356E-7	46.000004	6
CGGAACC	35	1.01954356E-7	46.000004	39
GATACCC	20	6.311411E-4	46.000004	39
CGAACGG	20	6.311411E-4	46.000004	2
TCCGCTA	20	6.311411E-4	46.000004	43
CGTATGG	75	0.0	46.000004	2
GATCGGA	35	1.01954356E-7	46.000004	8
CTCAACT	20	6.311411E-4	46.000004	12
GTCGAAT	40	5.6115823E-9	46.000004	46
TAAAGCG	35	1.01954356E-7	46.000004	1
ACGTTGT	20	6.311411E-4	46.000004	43
ACGCATC	20	6.311411E-4	46.000004	23
GCGGTAA	20	6.311411E-4	46.000004	46
GACACGG	40	5.6115823E-9	46.000004	2
TTTCGCG	35	1.01954356E-7	46.000004	1
CGTTGTA	20	6.311411E-4	46.000004	44
>>END_MODULE