FastQCFastQC Report
Thu 26 May 2016
SRR1544716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544716_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1349850
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62470.4627921620920843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47360.3508537985702115No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT18820.13942289884061193TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT18790.13920065192428788Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15220.11275326888172758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACA351.01974365E-746.00000436
ATTCGCA700.046.00000445
TCGTATT351.01974365E-746.00000419
ACTAACG700.046.0000041
CGGTCTT351.01974365E-746.00000417
AACCGTA206.311848E-446.032
CGGGTAC750.046.06
ACACGCG206.311848E-446.036
ACACGAA206.311848E-446.035
GTCGATA253.4174744E-546.022
GACACCG206.311848E-446.042
CATTGCG253.4174744E-546.01
ATTACCG206.311848E-446.01
AGGCGTA453.110472E-1046.019
CGATTCT206.311848E-446.012
TATGCGT253.4174744E-546.020
ACGGGTC405.6134013E-946.05
CCTCGAC253.4174744E-546.016
ATCCGTT253.4174744E-546.014
CGTTAGT301.8616465E-646.035
Produced by FastQC (version 0.11.5)