##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544716_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1349850
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.695000185205764	33.0	31.0	34.0	31.0	34.0
2	33.10010741934289	34.0	33.0	34.0	31.0	34.0
3	33.1417031522021	34.0	33.0	34.0	31.0	34.0
4	36.46824165648035	37.0	37.0	37.0	35.0	37.0
5	36.45775826943734	37.0	37.0	37.0	35.0	37.0
6	36.52454717190799	37.0	37.0	37.0	35.0	37.0
7	36.624986479979256	37.0	37.0	37.0	35.0	37.0
8	36.62442641775012	37.0	37.0	37.0	35.0	37.0
9	38.3546031040486	39.0	39.0	39.0	37.0	39.0
10	38.25999851835389	39.0	39.0	39.0	37.0	39.0
11	38.0688550579694	39.0	39.0	39.0	35.0	39.0
12	37.27265251694633	39.0	35.0	39.0	35.0	39.0
13	37.14972182094307	39.0	35.0	39.0	35.0	39.0
14	38.25513279253251	40.0	35.0	41.0	35.0	41.0
15	38.29381042338038	40.0	35.0	41.0	35.0	41.0
16	38.382587694929065	40.0	35.0	41.0	35.0	41.0
17	38.350888617253766	40.0	35.0	41.0	35.0	41.0
18	38.39685816942623	40.0	36.0	41.0	35.0	41.0
19	38.37323109975183	40.0	36.0	41.0	35.0	41.0
20	38.22594658665778	40.0	35.0	41.0	35.0	41.0
21	38.15248953587436	40.0	35.0	41.0	35.0	41.0
22	38.08851724265659	40.0	35.0	41.0	35.0	41.0
23	38.00606289587732	40.0	35.0	41.0	35.0	41.0
24	37.96548357224877	40.0	35.0	41.0	35.0	41.0
25	37.968423898951734	40.0	35.0	41.0	35.0	41.0
26	37.9082586954106	40.0	35.0	41.0	35.0	41.0
27	37.89859984442716	40.0	35.0	41.0	35.0	41.0
28	37.89474756454421	40.0	35.0	41.0	35.0	41.0
29	37.85062340260029	40.0	35.0	41.0	35.0	41.0
30	37.81466014742379	40.0	35.0	41.0	35.0	41.0
31	37.70271215320221	40.0	35.0	41.0	35.0	41.0
32	37.58143052931807	40.0	35.0	41.0	35.0	41.0
33	37.416017335259475	40.0	35.0	41.0	34.0	41.0
34	37.29728043856725	40.0	35.0	41.0	34.0	41.0
35	37.202641034188986	40.0	35.0	41.0	34.0	41.0
36	37.11190650813053	39.0	35.0	41.0	34.0	41.0
37	37.06741341630551	39.0	35.0	41.0	34.0	41.0
38	36.96587843093677	39.0	35.0	41.0	33.0	41.0
39	36.977259695521724	39.0	35.0	41.0	34.0	41.0
40	36.87102270622662	39.0	35.0	41.0	33.0	41.0
41	36.87272511760566	39.0	35.0	41.0	34.0	41.0
42	36.84767122272845	39.0	35.0	41.0	34.0	41.0
43	36.79747897914583	39.0	35.0	41.0	33.0	41.0
44	36.70347964588658	39.0	35.0	41.0	33.0	41.0
45	36.61153905989554	38.0	35.0	41.0	33.0	41.0
46	36.53773011816128	38.0	35.0	41.0	33.0	41.0
47	36.512588065340594	38.0	35.0	41.0	33.0	41.0
48	36.47506760010371	38.0	35.0	41.0	33.0	41.0
49	36.44647183020336	38.0	35.0	41.0	33.0	41.0
50	36.32461458680594	38.0	35.0	40.0	33.0	41.0
51	36.23711375338001	37.0	35.0	40.0	33.0	41.0
52	35.99192428788384	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	6.0
14	8.0
15	14.0
16	46.0
17	55.0
18	177.0
19	313.0
20	586.0
21	1013.0
22	1560.0
23	2612.0
24	4468.0
25	6988.0
26	9659.0
27	11068.0
28	11038.0
29	10976.0
30	11877.0
31	14501.0
32	18516.0
33	25696.0
34	66740.0
35	311567.0
36	53173.0
37	66083.0
38	128234.0
39	590843.0
40	2027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.521761677223395	24.578508723191465	29.03944882764752	13.860280771937623
2	30.601177908656517	24.68889135829907	30.59688113494092	14.113049598103494
3	27.600696373671145	25.95799533281476	32.692965885098346	13.74834240841575
4	25.24073045153165	27.847316368485387	32.10090010001112	14.811053079971847
5	22.55065377634552	33.19546616290699	29.650331518316854	14.60354854243064
6	21.100788976552952	41.82353594843872	26.266474052672518	10.809201022335815
7	87.80131125680631	4.428047560840094	6.079268066822239	1.6913731155313556
8	88.71741304589399	3.1822054302329885	6.039337704189355	2.0610438196836682
9	84.05467274141571	5.371411638330184	7.606548875801016	2.9673667444530873
10	48.257287846797794	28.890247064488644	12.85461347557136	9.9978516131422
11	43.58136089195096	20.48057191539801	23.2732525836204	12.664814609030634
12	42.15483201837241	21.863021817238952	23.183761158647258	12.798385005741379
13	20.055932140608217	41.67781605363559	24.09023224802756	14.176019557728637
14	13.685520613401488	42.786902248397965	26.823054413453345	16.704522724747196
15	12.294699411045672	26.17298218320554	48.36404044893877	13.168277956810016
16	14.510056672963662	20.646886691113828	47.39282142460273	17.450235211319775
17	15.634477904952401	20.898173871170872	29.47809015816572	33.989258065711006
18	18.05282068377968	23.61240137793088	38.327666036967074	20.007111901322368
19	26.573693373337775	23.462755120939363	28.82364707189688	21.139904433825983
20	29.09145460606734	23.051450161129015	30.359965922139498	17.497129310664146
21	19.734340852687335	30.425010186316996	31.26443678927288	18.576212171722783
22	21.633366670370783	23.638033855613585	26.129792199133238	28.598807274882393
23	15.968811349409192	31.841019372522872	26.691780568211282	25.498388709856652
24	15.184279734785347	23.070711560543764	44.11408675037967	17.630921954291217
25	15.09589954439382	26.518946549616622	38.59458458347223	19.790569322517317
26	14.139126569618846	35.579360669704045	29.443567803830057	20.837944956847057
27	14.766677779012483	40.493684483461124	28.132459162129127	16.607178575397267
28	13.969033596325517	30.4339741452754	38.45390228543912	17.143089972959956
29	14.848390561914288	24.401007519354	37.41089750713042	23.33970441160129
30	16.558802829944067	35.4273437789384	30.864021928362412	17.14983146275512
31	27.262732896247734	30.38663555209838	26.52694743860429	15.8236841130496
32	29.421565359113977	25.133014779419938	28.945216135126124	16.50020372633996
33	27.923991554617178	28.63733007371189	25.803459643664112	17.635218728006816
34	18.492573248879506	28.059117679742197	28.1266807423047	25.321628329073604
35	18.71422750676001	25.84109345482831	33.14805348742453	22.296625550987148
36	30.332851798347964	26.12445827314146	26.492202837352295	17.050487091158274
37	17.228729118050154	36.51664999814794	30.494721635737303	15.759899248064599
38	17.952587324517538	34.07260065933252	27.390747120050374	20.584064896099566
39	18.44864244175279	33.87280068155721	29.232507315627664	18.446049561062342
40	24.029336592954774	25.98666518502056	28.403822646960776	21.580175575063894
41	16.193058487980146	25.53965255398748	29.98503537430085	28.282253583731524
42	19.49557358224988	23.381116420343	28.130384857576768	28.99292513983035
43	20.153276289958143	24.649701818720597	29.173685965107232	26.023335926214024
44	16.512501389043226	29.38304256028448	31.004037485646553	23.100418565025745
45	16.012668074230472	40.09141756491462	25.159017668629847	18.73689669222506
46	19.702855872874764	35.76308478719858	26.433603733748196	18.100455606178464
47	18.65244286402193	28.833351853909694	28.241286068822465	24.272919213245917
48	18.992036152165056	26.47197836796681	35.36222543245546	19.173760047412674
49	21.640700818609478	25.96103270733785	34.03259621439419	18.365670259658483
50	19.496610734526058	34.17661221617217	28.226765936955957	18.100011112345815
51	17.220505982146165	33.25488017187095	26.279957032262846	23.244656813720045
52	17.49401785383561	27.93214060821573	35.98407230433011	18.58976923361855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5535.0
1	4931.0
2	4327.0
3	7461.5
4	10596.0
5	7283.0
6	3970.0
7	3992.5
8	4015.0
9	4312.5
10	4610.0
11	4537.5
12	4465.0
13	4498.5
14	4302.5
15	4073.0
16	4290.0
17	4507.0
18	4261.0
19	4015.0
20	5156.5
21	6298.0
22	6613.0
23	6928.0
24	7288.0
25	7648.0
26	10005.0
27	12362.0
28	17058.0
29	21754.0
30	21784.5
31	21815.0
32	27142.0
33	32469.0
34	37822.5
35	43176.0
36	49010.5
37	54845.0
38	60599.0
39	84727.5
40	103102.0
41	120353.0
42	137604.0
43	148077.5
44	158551.0
45	154180.0
46	149809.0
47	138356.5
48	126904.0
49	112701.0
50	98498.0
51	86112.5
52	73727.0
53	61972.5
54	50218.0
55	42531.5
56	34845.0
57	29316.5
58	23788.0
59	22176.5
60	20565.0
61	17280.5
62	13996.0
63	12091.5
64	8498.0
65	6809.0
66	5903.5
67	4998.0
68	3981.0
69	2964.0
70	2355.0
71	1746.0
72	1509.5
73	1273.0
74	1051.5
75	830.0
76	629.5
77	429.0
78	380.5
79	332.0
80	267.5
81	203.0
82	159.0
83	115.0
84	78.5
85	42.0
86	24.0
87	6.0
88	9.0
89	7.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1349850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.312453981313551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26893628393265	11.525521230701406
2	10.403826044534542	3.1861621507305316
3	3.6469871736719437	1.6753296980187724
4	1.760874753641454	1.078532545279664
5	1.0034093643621633	0.7682329858107354
6	0.6598278572990961	0.6062150220288679
7	0.48624434365924585	0.5211915895169349
8	0.37415493034130254	0.4583384122186218
9	0.3002361835144486	0.4137617469231184
>10	3.4668863055587904	12.613502435684909
>50	1.0308969395762562	11.126323220981636
>100	1.5694760681389202	52.36153665670615
>500	0.02532198434477935	2.356351171838699
>1k	0.0024348061869980146	0.8431891095583054
>5k	4.8696123739960285E-4	0.4658120240016567
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6247	0.4627921620920843	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4736	0.3508537985702115	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT	1882	0.13942289884061193	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1879	0.13920065192428788	Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1522	0.11275326888172758	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14579397710856762	0.0
2	0.0	0.0	0.0	0.46560728969885545	0.0
3	0.0	0.0	0.0	0.6265140571174574	0.0
4	0.0	0.0	0.0	0.8000888987665297	0.0
5	0.0	0.0	0.0	1.3024410119642924	0.0
6	0.0	0.0	0.0	1.6410712301366819	0.0
7	0.0	0.0	0.0	1.8733933400007408	0.0
8	0.0	0.0	0.0	2.365299848131274	0.0
9	0.0	0.0	0.0	2.543319628106827	0.0
10	0.0	0.0	0.0	2.922324702744749	0.0
11	0.0	0.0	0.0	3.435122421009742	0.0
12	0.0	0.0	0.0	3.8222024669407713	0.0
13	0.0	0.0	0.0	3.9742193577064118	0.0
14	0.0	0.0	0.0	4.031707226728896	0.0
15	0.0	0.0	0.0	4.130607104493092	0.0
16	0.0	0.0	0.0	4.362929214357151	0.0
17	0.0	0.0	0.0	4.642663999703671	0.0
18	0.0	0.0	0.0	5.037670852316924	0.0
19	0.0	0.0	0.0	5.221543134422343	0.0
20	0.0	0.0	0.0	5.400674148979516	0.0
21	0.0	0.0	0.0	5.647812719931844	0.0
22	0.0	0.0	0.0	5.896136607771234	0.0
23	0.0	0.0	0.0	6.181575730636737	0.0
24	1.4816461088269068E-4	0.0	0.0	6.400711190132237	0.0
25	1.4816461088269068E-4	0.0	0.0	6.5833981553505945	0.0
26	1.4816461088269068E-4	0.0	0.0	6.7646034744601256	0.0
27	1.4816461088269068E-4	0.0	0.0	6.932622143201097	0.0
28	1.4816461088269068E-4	0.0	0.0	7.104937585657666	0.0
29	1.4816461088269068E-4	0.0	0.0	7.294514205282068	0.0
30	1.4816461088269068E-4	0.0	0.0	7.538096825573212	0.0
31	1.4816461088269068E-4	0.0	0.0	7.77701226062155	0.0
32	1.4816461088269068E-4	0.0	0.0	7.9651813164425675	0.0
33	1.4816461088269068E-4	0.0	0.0	8.170537467125977	0.0
34	1.4816461088269068E-4	0.0	0.0	8.374634218616883	0.0
35	1.4816461088269068E-4	0.0	0.0	8.658221283846354	0.0
36	1.4816461088269068E-4	0.0	0.0	8.879505130199652	0.0
37	1.4816461088269068E-4	0.0	0.0	9.101455717301922	0.0
38	1.4816461088269068E-4	0.0	0.0	9.30399674037856	0.0
39	1.4816461088269068E-4	0.0	0.0	9.528836537393044	0.0
40	1.4816461088269068E-4	0.0	0.0	9.77093751157536	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACA	35	1.01974365E-7	46.000004	36
ATTCGCA	70	0.0	46.000004	45
TCGTATT	35	1.01974365E-7	46.000004	19
ACTAACG	70	0.0	46.000004	1
CGGTCTT	35	1.01974365E-7	46.000004	17
AACCGTA	20	6.311848E-4	46.0	32
CGGGTAC	75	0.0	46.0	6
ACACGCG	20	6.311848E-4	46.0	36
ACACGAA	20	6.311848E-4	46.0	35
GTCGATA	25	3.4174744E-5	46.0	22
GACACCG	20	6.311848E-4	46.0	42
CATTGCG	25	3.4174744E-5	46.0	1
ATTACCG	20	6.311848E-4	46.0	1
AGGCGTA	45	3.110472E-10	46.0	19
CGATTCT	20	6.311848E-4	46.0	12
TATGCGT	25	3.4174744E-5	46.0	20
ACGGGTC	40	5.6134013E-9	46.0	5
CCTCGAC	25	3.4174744E-5	46.0	16
ATCCGTT	25	3.4174744E-5	46.0	14
CGTTAGT	30	1.8616465E-6	46.0	35
>>END_MODULE