FastQCFastQC Report
Thu 26 May 2016
SRR1544715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544715_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1012260
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68950.68114911188825No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53410.5276312409855176No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT35500.35070041293738763TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT28040.2770039317961789TruSeq Adapter, Index 19 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19400.19165036650662873No Hit
GATGCGGGGGATTGAACTTCTTCCTAACCCGTGTTAACACCACTGAATGTGT15270.15085057198743404No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT12720.12565941556517102TruSeq Adapter, Index 19 (95% over 22bp)
TGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA11910.11765751881927568No Hit
AAAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT11530.1139035425681149No Hit
GTGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT10930.10797621164522948No Hit
TTTCTGGGGATTTACATTGTTACTTTAAATGCCTTGGAATTTGAGGCTTACC10150.10027068144547843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG253.4168654E-546.01
CTAGAGT206.311095E-446.017
CTAGACG206.311095E-446.01
AGCCGTA405.6097633E-946.036
CGGAACG206.311095E-446.06
TCGTTAG301.8611809E-646.01
AGTCCCG253.4168654E-546.035
TACCCCG253.4168654E-546.043
ACACGAC206.311095E-446.026
CGAACAC253.4168654E-546.025
TCCGCTA206.311095E-446.02
GCGACAG253.4168654E-546.01
CATATAG405.6097633E-946.01
TCCGCAT206.311095E-446.023
TCACCCG253.4168654E-546.033
GATCGCG405.6097633E-946.01
GTACAAG453.092282E-1046.01
CAACGAG405.6097633E-946.01
CGCGGCC551.8189894E-1246.035
GCGAATC206.311095E-446.017
Produced by FastQC (version 0.11.5)