##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544715_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1012260
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67130183944836	33.0	31.0	34.0	31.0	34.0
2	33.06979728528244	34.0	33.0	34.0	31.0	34.0
3	33.109866042321144	34.0	33.0	34.0	31.0	34.0
4	36.43321083516093	37.0	37.0	37.0	35.0	37.0
5	36.4198535949262	37.0	37.0	37.0	35.0	37.0
6	36.49167111216486	37.0	37.0	37.0	35.0	37.0
7	36.604980933752195	37.0	37.0	37.0	35.0	37.0
8	36.605757414103095	37.0	37.0	37.0	35.0	37.0
9	38.32472981249877	39.0	39.0	39.0	37.0	39.0
10	38.22190445142552	39.0	39.0	39.0	37.0	39.0
11	37.9977861419003	39.0	39.0	39.0	35.0	39.0
12	37.0692006006362	39.0	35.0	39.0	35.0	39.0
13	36.89912967024282	39.0	35.0	39.0	35.0	39.0
14	37.952741390551836	40.0	35.0	41.0	35.0	41.0
15	38.03038251042222	40.0	35.0	41.0	35.0	41.0
16	38.13115800288463	40.0	35.0	41.0	35.0	41.0
17	38.08373145239366	40.0	35.0	41.0	35.0	41.0
18	38.129332384960385	39.0	36.0	41.0	35.0	41.0
19	38.08314662240926	39.0	36.0	41.0	35.0	41.0
20	37.90959437298718	39.0	35.0	41.0	35.0	41.0
21	37.8194386817616	39.0	35.0	41.0	35.0	41.0
22	37.764351056052796	39.0	35.0	41.0	35.0	41.0
23	37.71885187600024	39.0	35.0	41.0	35.0	41.0
24	37.680943631082926	39.0	35.0	41.0	35.0	41.0
25	37.66129650485053	39.0	35.0	41.0	35.0	41.0
26	37.603174085709206	39.0	35.0	41.0	35.0	41.0
27	37.59078003674945	39.0	35.0	41.0	35.0	41.0
28	37.5776401319819	39.0	35.0	41.0	35.0	41.0
29	37.529386718827176	39.0	35.0	41.0	35.0	41.0
30	37.510245391500206	38.0	35.0	41.0	35.0	41.0
31	37.36780273842689	39.0	35.0	41.0	35.0	41.0
32	37.196400134352835	38.0	35.0	41.0	34.0	41.0
33	37.054643075889594	38.0	35.0	41.0	34.0	41.0
34	36.93405943137139	38.0	35.0	41.0	34.0	41.0
35	36.81892102819434	38.0	35.0	41.0	34.0	41.0
36	36.72437713630885	38.0	35.0	41.0	33.0	41.0
37	36.67173058305178	38.0	35.0	41.0	33.0	41.0
38	36.566358445458675	38.0	35.0	41.0	33.0	41.0
39	36.577755714934895	38.0	35.0	41.0	33.0	41.0
40	36.44803015035663	38.0	35.0	41.0	32.0	41.0
41	36.47862999624602	38.0	35.0	41.0	33.0	41.0
42	36.48858099697706	38.0	35.0	41.0	33.0	41.0
43	36.41100310196985	38.0	35.0	41.0	33.0	41.0
44	36.32154683579318	37.0	35.0	41.0	33.0	41.0
45	36.262948254401046	37.0	35.0	41.0	33.0	41.0
46	36.22984707486219	37.0	35.0	41.0	33.0	41.0
47	36.1620828640863	37.0	35.0	41.0	33.0	41.0
48	36.136605220002764	37.0	35.0	41.0	33.0	41.0
49	36.14669057356806	37.0	35.0	40.0	33.0	41.0
50	36.019360638571115	37.0	35.0	40.0	33.0	41.0
51	35.941977357595874	36.0	35.0	40.0	32.0	41.0
52	35.68646197617213	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	2.0
13	1.0
14	2.0
15	6.0
16	27.0
17	63.0
18	172.0
19	271.0
20	528.0
21	845.0
22	1380.0
23	2309.0
24	3921.0
25	6261.0
26	8123.0
27	8744.0
28	8675.0
29	8754.0
30	9860.0
31	11989.0
32	14955.0
33	20997.0
34	57155.0
35	271693.0
36	38995.0
37	46202.0
38	82501.0
39	405989.0
40	1836.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.911030762847492	24.36705984628455	30.218323355659614	14.503586035208347
2	27.094916325845137	27.30296564123842	30.971687115958353	14.630430916958092
3	27.17019342856578	26.421176377610493	32.90231758639085	13.506312607432875
4	25.679568490308814	28.90206073538419	30.882974729812503	14.535396044494497
5	22.119020804931537	33.71416434512872	29.768735305158756	14.398079544780984
6	21.639697310967538	42.592219390275226	25.154802125936026	10.613281172821212
7	87.12583723549285	5.296465334992987	5.753067393752593	1.8246300357615632
8	87.86220931381266	3.9252761148321573	5.89255724813783	2.319957323217355
9	84.0179400549266	5.631853476379586	7.355521308754668	2.994685159939146
10	50.32076739177682	29.527690514294747	11.433031039456267	8.71851105447217
11	46.59425444055875	19.434137474561872	21.51621125007409	12.455396834805287
12	43.69648114120878	20.945804437595083	22.93719004998716	12.420524371208979
13	19.13757335072017	46.152865864501216	22.775571493489817	11.9339892912888
14	12.255744571552764	48.67464880564282	25.525161519767646	13.54444510303677
15	10.341809416553058	25.567838302412422	51.746290478730764	12.344061802303756
16	11.949202773990871	21.076798450990854	49.9906150593721	16.983383715646177
17	12.725584336040146	20.370952126923914	28.58939402920198	38.314069507833956
18	15.937209807756899	24.248710805524272	40.35662774386027	19.457451642858555
19	26.62082864086302	24.007863592357694	28.670796040542946	20.700511726236343
20	28.946416928457115	22.947365301404776	29.323493964001347	18.782723806136765
21	17.66068006243455	31.26844881749748	31.817616027502815	19.25325509256515
22	20.06124908620315	23.51935273546322	25.828048129927094	30.591350048406536
23	14.509612154979944	31.765455515381426	26.306778890798803	27.418153438839827
24	14.32635884061407	22.654653942662954	45.741509098452966	17.27747811827001
25	12.642107758876179	27.01973801197321	40.95815304368443	19.380001185466185
26	12.823780451662618	38.40268310513109	28.332444233694897	20.441092209511392
27	12.990042084049552	42.4962954181732	27.550332918420168	16.96332957935708
28	11.274178570722936	33.53871534981131	38.23148203030841	16.95562404915733
29	11.784818129729516	25.747337640527135	37.339023571019304	25.128820658724045
30	14.030683816410802	37.17424377136309	30.658032521288998	18.13703989093711
31	26.721593266552073	31.471064746211447	25.62138185841582	16.18596012882066
32	28.00624345523877	27.734870487819336	28.32928299053603	15.929603066405864
33	27.85667713828463	29.46298381838658	23.955999446782446	18.72433959654634
34	16.86108312093731	29.636061881334836	26.401221030170113	27.101633967557742
35	15.922292691600973	27.058364451820676	32.48839231027601	24.530950546302332
36	30.545116867208026	25.652994290004543	25.94027226206706	17.86161658072037
37	16.271708849505067	37.29377827830794	29.39314010234525	17.04137276984174
38	17.251002706814454	35.71058818880525	24.41665184833936	22.62175725604094
39	18.08448422342086	34.984193784205644	27.902712741785706	19.028609250587795
40	23.812656827297335	26.614703732242706	25.951632979669252	23.621006460790706
41	13.899195858771463	25.303973287495307	29.128385987789695	31.668444865943535
42	19.18775808586727	23.451583585244897	27.013217947958033	30.347440380929804
43	19.55554896963231	23.814435026574202	29.404698397644875	27.225317606148618
44	14.712129294845196	30.030525754252864	32.3771560666232	22.880188884278745
45	13.886946041530832	42.76025922193903	23.262798095351	20.089996641179145
46	20.36176476399344	35.73508782328651	25.997964949716472	17.905182463003577
47	17.305336573607573	30.71345306541798	26.316657775670283	25.664552585304172
48	19.105466974887875	26.26805366210262	33.55175547784166	21.074723885167842
49	21.83944836306878	23.714658289372295	35.34180941655306	19.10408393100587
50	19.21571533005354	35.85254776440836	27.251496651058027	17.680240254480072
51	16.902179282002646	34.11425918242349	24.72645367790884	24.257107857665027
52	17.28044178373145	27.782585501748564	36.04735937407386	18.88961334044613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7534.0
1	5875.0
2	4216.0
3	6336.0
4	8456.0
5	5693.5
6	2931.0
7	3128.0
8	3325.0
9	3663.5
10	4002.0
11	4081.0
12	4160.0
13	4232.0
14	4139.5
15	3975.0
16	3896.5
17	3818.0
18	3367.5
19	2917.0
20	3564.5
21	4212.0
22	4215.0
23	4218.0
24	5808.0
25	7398.0
26	8089.5
27	8781.0
28	10801.0
29	12821.0
30	14556.5
31	16292.0
32	19175.5
33	22059.0
34	24207.5
35	26356.0
36	30499.0
37	34642.0
38	39637.5
39	59013.5
40	73394.0
41	91792.0
42	110190.0
43	119011.0
44	127832.0
45	124797.5
46	121763.0
47	112312.0
48	102861.0
49	93084.5
50	83308.0
51	68443.0
52	53578.0
53	46382.0
54	39186.0
55	31389.0
56	23592.0
57	19808.0
58	16024.0
59	13486.5
60	10949.0
61	8747.5
62	6546.0
63	5579.5
64	3544.0
65	2475.0
66	2050.5
67	1626.0
68	1386.5
69	1147.0
70	974.0
71	801.0
72	749.0
73	697.0
74	456.5
75	216.0
76	203.0
77	190.0
78	175.0
79	160.0
80	95.0
81	30.0
82	23.5
83	17.0
84	12.5
85	8.0
86	5.0
87	2.0
88	2.5
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1012260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.760939641454947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54269347724647	12.326446464421508
2	11.308483372606313	3.7908161448930255
3	4.292939037863061	2.158610762942053
4	2.1145592383830585	1.4176799905127757
5	1.2157788851543827	1.0188798255714
6	0.7525396252078007	0.7567962741546647
7	0.6012127224153462	0.7053823106454897
8	0.42925748729020496	0.5755807068090992
9	0.3174086508866315	0.4788060515267828
>10	3.0795692749188155	11.938027127320103
>50	0.9158451738413118	10.8799490917841
>100	1.4107686162015811	49.80860877002105
>500	0.012432287427710116	1.387789615745916
>1k	0.005328123183304335	1.542484597223671
>5k	0.00118402737406763	1.2141422664283588
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6895	0.68114911188825	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5341	0.5276312409855176	No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT	3550	0.35070041293738763	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	2804	0.2770039317961789	TruSeq Adapter, Index 19 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1940	0.19165036650662873	No Hit
GATGCGGGGGATTGAACTTCTTCCTAACCCGTGTTAACACCACTGAATGTGT	1527	0.15085057198743404	No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	1272	0.12565941556517102	TruSeq Adapter, Index 19 (95% over 22bp)
TGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1191	0.11765751881927568	No Hit
AAAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	1153	0.1139035425681149	No Hit
GTGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	1093	0.10797621164522948	No Hit
TTTCTGGGGATTTACATTGTTACTTTAAATGCCTTGGAATTTGAGGCTTACC	1015	0.10027068144547843	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3686799834034734	0.0
2	0.0	0.0	0.0	0.9874933317527117	0.0
3	0.0	0.0	0.0	1.298875782901626	0.0
4	0.0	0.0	0.0	1.639697310967538	0.0
5	0.0	0.0	0.0	2.5590263371070674	0.0
6	0.0	0.0	0.0	3.162231047359374	0.0
7	0.0	0.0	0.0	3.5785272558433605	0.0
8	0.0	0.0	0.0	4.37387627684587	0.0
9	9.878884871475707E-5	0.0	0.0	4.647027443542173	0.0
10	9.878884871475707E-5	0.0	0.0	5.2208918657261965	0.0
11	9.878884871475707E-5	0.0	0.0	5.976922924940233	0.0
12	9.878884871475707E-5	0.0	0.0	6.611937644478691	0.0
13	9.878884871475707E-5	0.0	0.0	6.846462371327524	0.0
14	9.878884871475707E-5	0.0	0.0	6.93359413589394	0.0
15	9.878884871475707E-5	0.0	0.0	7.084938652124948	0.0
16	9.878884871475707E-5	0.0	0.0	7.416078873016814	0.0
17	9.878884871475707E-5	0.0	0.0	7.822594985478039	0.0
18	9.878884871475707E-5	0.0	0.0	8.415130500069152	0.0
19	9.878884871475707E-5	0.0	0.0	8.667437219686642	0.0
20	9.878884871475707E-5	0.0	0.0	8.919052417363128	0.0
21	9.878884871475707E-5	0.0	0.0	9.253156303716436	0.0
22	9.878884871475707E-5	0.0	0.0	9.587556556615889	0.0
23	9.878884871475707E-5	0.0	0.0	10.000889099638433	0.0
24	9.878884871475707E-5	0.0	0.0	10.289056171339379	0.0
25	9.878884871475707E-5	0.0	0.0	10.50836741548614	0.0
26	9.878884871475707E-5	0.0	0.0	10.731926580127634	0.0
27	9.878884871475707E-5	0.0	0.0	10.934740086539032	0.0
28	1.9757769742951415E-4	0.0	0.0	11.15069250982949	0.0
29	1.9757769742951415E-4	0.0	0.0	11.398948886649675	0.0
30	1.9757769742951415E-4	0.0	0.0	11.730780629482544	0.0
31	1.9757769742951415E-4	0.0	0.0	12.035643016616284	0.0
32	1.9757769742951415E-4	0.0	0.0	12.274020508564993	0.0
33	1.9757769742951415E-4	0.0	0.0	12.521782941141604	0.0
34	1.9757769742951415E-4	0.0	0.0	12.774583605002666	0.0
35	1.9757769742951415E-4	0.0	0.0	13.161341947720942	0.0
36	1.9757769742951415E-4	0.0	0.0	13.419477209412602	0.0
37	1.9757769742951415E-4	0.0	0.0	13.686207100942445	0.0
38	1.9757769742951415E-4	0.0	0.0	13.935154999703633	0.0
39	1.9757769742951415E-4	0.0	0.0	14.186572619682691	0.0
40	1.9757769742951415E-4	0.0	0.0	14.486693142078122	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	3.4168654E-5	46.0	1
CTAGAGT	20	6.311095E-4	46.0	17
CTAGACG	20	6.311095E-4	46.0	1
AGCCGTA	40	5.6097633E-9	46.0	36
CGGAACG	20	6.311095E-4	46.0	6
TCGTTAG	30	1.8611809E-6	46.0	1
AGTCCCG	25	3.4168654E-5	46.0	35
TACCCCG	25	3.4168654E-5	46.0	43
ACACGAC	20	6.311095E-4	46.0	26
CGAACAC	25	3.4168654E-5	46.0	25
TCCGCTA	20	6.311095E-4	46.0	2
GCGACAG	25	3.4168654E-5	46.0	1
CATATAG	40	5.6097633E-9	46.0	1
TCCGCAT	20	6.311095E-4	46.0	23
TCACCCG	25	3.4168654E-5	46.0	33
GATCGCG	40	5.6097633E-9	46.0	1
GTACAAG	45	3.092282E-10	46.0	1
CAACGAG	40	5.6097633E-9	46.0	1
CGCGGCC	55	1.8189894E-12	46.0	35
GCGAATC	20	6.311095E-4	46.0	17
>>END_MODULE