SRR1544714_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544714_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1090283
Sequences flagged as poor quality	0
Sequence length	52
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5139	0.47134551304569544	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4737	0.4344743520718932	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	2433	0.22315307126681785	TruSeq Adapter, Index 10 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2060	0.18894177016426011	RNA PCR Primer, Index 10 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1509	0.13840443261061577	No Hit
TGTGTAGGGGGTGATAGAGAACTATTTTTTAAAATGTTTTCTTTCTGTTTCA	1279	0.11730899225247023	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	35	1.0195072E-7	46.000004	42
GCGAGAC	20	6.3113094E-4	46.000004	21
AACGTCA	20	6.3113094E-4	46.000004	27
CGTGGCC	20	6.3113094E-4	46.000004	41
TCGTTCC	20	6.3113094E-4	46.000004	24
TCGTTAG	20	6.3113094E-4	46.000004	1
ACCGGTG	40	5.6115823E-9	46.000004	41
ACACGTG	20	6.3113094E-4	46.000004	42
TACCCCG	40	5.6115823E-9	46.000004	25
ACCGGGA	70	0.0	46.000004	4
CCCTCGT	20	6.3113094E-4	46.000004	23
GTCGATG	80	0.0	46.000004	1
ATCTACG	20	6.3113094E-4	46.000004	1
CGAAACG	20	6.3113094E-4	46.000004	40
ACGCAGC	20	6.3113094E-4	46.000004	30
GCGAAGC	20	6.3113094E-4	46.000004	44
GACACGA	35	1.0195072E-7	46.000004	25
TGGACGA	35	1.0195072E-7	46.000004	40
CGGCCAG	20	6.3113094E-4	46.000004	32
ACACCGA	20	6.3113094E-4	46.000004	37