##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544714_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1090283
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71505012918664	33.0	31.0	34.0	31.0	34.0
2	33.121259342757796	34.0	33.0	34.0	31.0	34.0
3	33.154480992549644	34.0	33.0	34.0	31.0	34.0
4	36.463188915171564	37.0	37.0	37.0	35.0	37.0
5	36.45082148396334	37.0	37.0	37.0	35.0	37.0
6	36.52081065191331	37.0	37.0	37.0	35.0	37.0
7	36.64143346268813	37.0	37.0	37.0	35.0	37.0
8	36.65340374930179	37.0	37.0	37.0	35.0	37.0
9	38.4334305863707	39.0	39.0	39.0	37.0	39.0
10	38.291584845402525	39.0	39.0	39.0	37.0	39.0
11	38.024184546581026	39.0	39.0	39.0	35.0	39.0
12	37.088686148458706	39.0	35.0	39.0	35.0	39.0
13	36.96761116150577	39.0	35.0	39.0	35.0	39.0
14	38.00975893414829	40.0	35.0	41.0	35.0	41.0
15	38.06207287465731	40.0	35.0	41.0	35.0	41.0
16	38.15630253796491	40.0	35.0	41.0	35.0	41.0
17	38.11131421841852	40.0	35.0	41.0	35.0	41.0
18	38.16980086821495	40.0	36.0	41.0	35.0	41.0
19	38.14430290117337	40.0	36.0	41.0	35.0	41.0
20	37.97912101720379	40.0	35.0	41.0	35.0	41.0
21	37.89092923580392	40.0	35.0	41.0	35.0	41.0
22	37.81130678915474	39.0	35.0	41.0	35.0	41.0
23	37.75769593766022	39.0	35.0	41.0	35.0	41.0
24	37.705720441389985	39.0	35.0	41.0	35.0	41.0
25	37.72023502154945	39.0	35.0	41.0	35.0	41.0
26	37.650305471148314	39.0	35.0	41.0	35.0	41.0
27	37.63740790235196	39.0	35.0	41.0	35.0	41.0
28	37.67548242061923	39.0	35.0	41.0	35.0	41.0
29	37.67562183396421	39.0	35.0	41.0	35.0	41.0
30	37.62711883061554	39.0	35.0	41.0	35.0	41.0
31	37.466827420036815	39.0	35.0	41.0	35.0	41.0
32	37.333806910682824	39.0	35.0	41.0	35.0	41.0
33	37.20705541588743	39.0	35.0	41.0	34.0	41.0
34	37.063462422141775	39.0	35.0	41.0	34.0	41.0
35	36.9543980782971	38.0	35.0	41.0	34.0	41.0
36	36.86579814598595	38.0	35.0	41.0	34.0	41.0
37	36.821004271368075	38.0	35.0	41.0	34.0	41.0
38	36.73153942600224	38.0	35.0	41.0	33.0	41.0
39	36.77019727905507	38.0	35.0	41.0	34.0	41.0
40	36.657064266800454	38.0	35.0	41.0	33.0	41.0
41	36.67227499649174	38.0	35.0	41.0	34.0	41.0
42	36.68936413756795	38.0	35.0	41.0	34.0	41.0
43	36.61032043973904	38.0	35.0	41.0	33.0	41.0
44	36.547063468842495	38.0	35.0	41.0	34.0	41.0
45	36.462044258233874	38.0	35.0	41.0	33.0	41.0
46	36.431372405146185	38.0	35.0	41.0	33.0	41.0
47	36.38408559979381	37.0	35.0	41.0	33.0	41.0
48	36.35100244615389	37.0	35.0	41.0	33.0	41.0
49	36.348253618555916	37.0	35.0	40.0	33.0	41.0
50	36.221079297760305	37.0	35.0	40.0	33.0	41.0
51	36.13994898572206	37.0	35.0	40.0	33.0	41.0
52	35.88055761669218	36.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	0.0
14	2.0
15	7.0
16	17.0
17	42.0
18	133.0
19	246.0
20	445.0
21	790.0
22	1162.0
23	2075.0
24	3610.0
25	6038.0
26	8245.0
27	9174.0
28	8512.0
29	8697.0
30	9518.0
31	11303.0
32	14518.0
33	21290.0
34	58144.0
35	297394.0
36	42328.0
37	45287.0
38	86592.0
39	452780.0
40	1931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.620123399154163	24.828782985701878	30.679282351462877	13.87181126368108
2	28.76785201640308	27.044721416366208	30.790904746749238	13.396521820481471
3	28.914511186545145	25.040195985812858	33.27292088384392	12.772371943798078
4	24.248841814464686	29.236262511659817	32.17522423077311	14.339671443102386
5	23.509492489564636	33.26063049685265	29.02182277445397	14.208054239128739
6	20.06717521964481	41.45538360223905	26.6450086812323	11.832432496883836
7	88.77080537805323	4.042620127067926	5.487657791600896	1.6989167032779564
8	89.70138945576515	2.880169644028202	5.441431261424786	1.9770096387818576
9	86.29961211905533	4.131312695878043	6.892889277371104	2.6761859076955252
10	55.697098826634914	24.736788521879184	11.308806979472303	8.257305672013597
11	46.446839948894	19.32434056112037	22.325763127554957	11.903056362430672
12	43.650318311851144	20.962447364583323	23.337151913769176	12.050082409796357
13	19.292972558500868	45.445540286329326	23.49206582144269	11.769421333727115
14	11.84206302400386	47.42686073248872	27.306304876807214	13.424771366700206
15	10.075916069497552	25.4516487921026	52.508201998930545	11.964233139469293
16	12.304144887153152	19.89134931022496	51.54001300579758	16.26449279682431
17	13.203636120163297	19.309757191481477	29.82363294667531	37.66297374167991
18	16.6878691128817	23.93130957742164	39.64530310020426	19.735518209492398
19	26.46487196443492	24.333773891732697	28.96559883993422	20.235755303898163
20	30.227748208492656	23.31789085952913	28.27256776451619	18.181793167462025
21	19.293155997112677	31.013507502180627	30.862537524661025	18.830798976045667
22	19.300676980196883	24.410359512163357	25.642883544914486	30.646079962725274
23	15.167988494730267	31.531996738461483	26.389386975675123	26.91062779113313
24	14.953273599606709	21.639427561467986	46.26193382818956	17.145365010735745
25	13.851999893605605	25.277565549494945	41.2260853374766	19.644349219422846
26	13.424404489476585	37.90694709538716	29.095840254319295	19.57280816081696
27	13.470447581040887	42.51850207698368	28.253581868193855	15.757468473781577
28	12.245903127903489	32.24227104338965	39.64456934575702	15.86725648294984
29	12.31515120386175	24.843458074646673	39.26063233123877	23.580758390252807
30	15.64630467502474	36.043302518703854	31.932351508736723	16.378041297534676
31	27.067926400760168	30.796683063021252	26.558333937152096	15.57705659906648
32	28.423904619259403	25.162916417113724	31.364425566573082	15.048753397053794
33	28.943035890681596	29.17040805002004	24.88280565687991	17.003750402418454
34	18.173997026460103	28.4920520635468	27.75985684450734	25.57409406548575
35	17.17746676780249	26.2969339153229	34.356767921723076	22.168831395151535
36	31.517505088128495	24.504096642798245	27.77123003843956	16.207168230633698
37	17.497658864716776	36.87666413215651	30.2561811933232	15.36949580980351
38	17.019801280951828	35.555447530595266	26.681879842206108	20.7428713462468
39	18.934625230330106	33.891109005643486	29.04777933802508	18.126486426001325
40	24.27213851816455	24.796589509329227	29.049980601366805	21.88129137113942
41	14.06093647245715	25.771565731099173	30.409627592102233	29.75787020434144
42	19.314710034000345	23.0779531552817	27.835250113961234	29.772086696756713
43	19.414225480907252	23.636156851019415	29.83518957921934	27.11442808885399
44	15.848820902462938	29.78877961043142	32.73416168095806	21.62823780614758
45	14.399105553328814	41.7987806835473	24.989658648259212	18.812455114864672
46	20.485873851101044	36.30424394400353	26.015997681335946	17.19388452355948
47	17.786024362482035	29.708525217764564	27.731882456206325	24.77356796354708
48	20.00535640746485	25.217581123433092	35.19554097422412	19.581521494877936
49	21.550092957516533	24.16776194804468	35.53802086247332	18.74412423196546
50	19.613714971250584	35.734116738498166	27.067284365618832	17.58488392463241
51	16.99687145447558	34.86388396407171	25.31608765797504	22.823156923477665
52	17.48298377577198	26.790108623173985	37.498979622721805	18.227927978332232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5739.0
1	4588.0
2	3437.0
3	5721.0
4	8005.0
5	5396.5
6	2788.0
7	2981.5
8	3175.0
9	3428.5
10	3682.0
11	3797.0
12	3912.0
13	3925.5
14	3884.5
15	3830.0
16	3655.0
17	3480.0
18	3797.5
19	4115.0
20	3705.5
21	3296.0
22	3659.5
23	4023.0
24	5430.0
25	6837.0
26	8116.0
27	9395.0
28	11897.5
29	14400.0
30	18183.0
31	21966.0
32	25389.5
33	28813.0
34	31193.5
35	33574.0
36	37890.5
37	42207.0
38	47888.5
39	66059.0
40	78548.0
41	103569.5
42	128591.0
43	135774.5
44	142958.0
45	139391.0
46	135824.0
47	119030.0
48	102236.0
49	87539.5
50	72843.0
51	64146.5
52	55450.0
53	45778.5
54	36107.0
55	29798.0
56	23489.0
57	20439.5
58	17390.0
59	15208.0
60	13026.0
61	10607.0
62	8188.0
63	6728.5
64	3901.0
65	2533.0
66	1992.5
67	1452.0
68	1120.5
69	789.0
70	619.0
71	449.0
72	423.5
73	398.0
74	312.0
75	226.0
76	214.0
77	202.0
78	122.5
79	43.0
80	48.5
81	54.0
82	40.5
83	27.0
84	17.0
85	7.0
86	4.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1090283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.62141202901307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23839812040136	11.597086054126992
2	10.82599120251844	3.3823453839402244
3	4.003999232170346	1.8764436530875486
4	1.918586833046307	1.198841417298227
5	1.1704640871508591	0.9142150885273118
6	0.759164124413997	0.7115529351069594
7	0.5106303787021258	0.5583737278165822
8	0.3994460978209411	0.4991929661953905
9	0.31521581313444186	0.4431704485530157
>10	3.2225986383525855	11.792285576102456
>50	1.0362623397534334	11.445182159681696
>100	1.5726874347624715	51.754757334577974
>500	0.023014938069694704	2.2449161595174396
>1k	0.0029506330858582955	1.1078926743216404
>5k	5.901266171716591E-4	0.47374442114652277
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5139	0.47134551304569544	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4737	0.4344743520718932	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	2433	0.22315307126681785	TruSeq Adapter, Index 10 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2060	0.18894177016426011	RNA PCR Primer, Index 10 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1509	0.13840443261061577	No Hit
TGTGTAGGGGGTGATAGAGAACTATTTTTTAAAATGTTTTCTTTCTGTTTCA	1279	0.11730899225247023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23250844046912592	0.0
2	0.0	0.0	0.0	0.644052966064774	0.0
3	0.0	0.0	0.0	0.8691321427555965	0.0
4	0.0	0.0	0.0	1.1167742686990443	0.0
5	0.0	0.0	0.0	1.781005482062914	0.0
6	0.0	0.0	0.0	2.2533599074735644	0.0
7	0.0	0.0	0.0	2.5849251983200694	0.0
8	0.0	0.0	0.0	3.277039080679053	0.0
9	0.0	0.0	0.0	3.5219296274453513	0.0
10	0.0	0.0	0.0	3.977774577793105	0.0
11	0.0	0.0	0.0	4.598255682240299	0.0
12	0.0	0.0	0.0	5.087303021325655	0.0
13	0.0	0.0	0.0	5.265788790616748	0.0
14	9.171930590498063E-5	0.0	0.0	5.32641525181994	0.0
15	9.171930590498063E-5	0.0	0.0	5.4302415061043785	0.0
16	9.171930590498063E-5	0.0	0.0	5.696227493228823	0.0
17	9.171930590498063E-5	0.0	0.0	6.009999238729761	0.0
18	9.171930590498063E-5	0.0	0.0	6.458323205993306	0.0
19	9.171930590498063E-5	0.0	0.0	6.674780767929061	0.0
20	9.171930590498063E-5	0.0	0.0	6.880598890379837	0.0
21	4.5859652952490317E-4	0.0	0.0	7.1834560384780834	0.0
22	4.5859652952490317E-4	0.0	0.0	7.48246097572832	0.0
23	4.5859652952490317E-4	0.0	0.0	7.83824016333374	0.0
24	8.254737531448257E-4	0.0	0.0	8.083497587323658	0.0
25	9.171930590498063E-4	0.0	0.0	8.267578234274954	0.0
26	9.171930590498063E-4	0.0	0.0	8.47413011117297	0.0
27	9.171930590498063E-4	0.0	0.0	8.657110126453407	0.0
28	9.171930590498063E-4	0.0	0.0	8.839723264510223	0.0
29	9.171930590498063E-4	0.0	0.0	9.042698088477946	0.0
30	9.171930590498063E-4	0.0	0.0	9.339410043080559	0.0
31	9.171930590498063E-4	0.0	0.0	9.598425362956224	0.0
32	9.171930590498063E-4	0.0	0.0	9.819744048104942	0.0
33	9.171930590498063E-4	0.0	0.0	10.029322662097822	0.0
34	9.171930590498063E-4	0.0	0.0	10.265866752026767	0.0
35	9.171930590498063E-4	0.0	0.0	10.581839760869425	0.0
36	0.001008912364954787	0.0	0.0	10.81746665773932	0.0
37	0.001008912364954787	0.0	0.0	11.065934257435913	0.0
38	0.001008912364954787	0.0	0.0	11.311650277955357	0.0
39	0.001008912364954787	0.0	0.0	11.544617314954007	0.0
40	0.001008912364954787	0.0	0.0	11.808310319430827	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	35	1.0195072E-7	46.000004	42
GCGAGAC	20	6.3113094E-4	46.000004	21
AACGTCA	20	6.3113094E-4	46.000004	27
CGTGGCC	20	6.3113094E-4	46.000004	41
TCGTTCC	20	6.3113094E-4	46.000004	24
TCGTTAG	20	6.3113094E-4	46.000004	1
ACCGGTG	40	5.6115823E-9	46.000004	41
ACACGTG	20	6.3113094E-4	46.000004	42
TACCCCG	40	5.6115823E-9	46.000004	25
ACCGGGA	70	0.0	46.000004	4
CCCTCGT	20	6.3113094E-4	46.000004	23
GTCGATG	80	0.0	46.000004	1
ATCTACG	20	6.3113094E-4	46.000004	1
CGAAACG	20	6.3113094E-4	46.000004	40
ACGCAGC	20	6.3113094E-4	46.000004	30
GCGAAGC	20	6.3113094E-4	46.000004	44
GACACGA	35	1.0195072E-7	46.000004	25
TGGACGA	35	1.0195072E-7	46.000004	40
CGGCCAG	20	6.3113094E-4	46.000004	32
ACACCGA	20	6.3113094E-4	46.000004	37
>>END_MODULE