FastQCFastQC Report
Thu 26 May 2016
SRR1544713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544713_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences865362
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41810.4831504041083384No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34200.3952103281632427No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT14540.1680221687571213TruSeq Adapter, Index 16 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.14352375075401969No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT11790.13624356049838102TruSeq Adapter, Index 13 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGA351.01917976E-746.00000428
GTACCGC700.046.00000431
GCCCAAT351.01917976E-746.0000049
AGTCACG351.01917976E-746.0000041
GTCGAAA351.01917976E-746.00000417
ATTACGT351.01917976E-746.0000048
CAACCTA351.01917976E-746.00000424
TGAACGG700.046.0000042
CGACACT351.01917976E-746.00000425
AGGCGAG351.01917976E-746.0000041
GCCGATG351.01917976E-746.0000049
CCCCGAA351.01917976E-746.00000429
ACCAGTA351.01917976E-746.00000410
TCGTAGT351.01917976E-746.00000422
ACGTCCA351.01917976E-746.00000411
TCGACAC351.01917976E-746.00000424
ACGCGCA351.01917976E-746.00000435
TCACGGT206.3105865E-446.046
CTTAACG253.4164506E-546.016
CGGGTGA206.3105865E-446.06
Produced by FastQC (version 0.11.5)