FastQCFastQC Report
Thu 26 May 2016
SRR1544712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544712_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1040496
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.5028371084559671No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31530.3030285556119389No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA551.8189894E-1246.017
TCGCAAA206.311178E-446.033
GTTTCGA301.8612318E-646.09
ACGCACC301.8612318E-646.029
TAGGTCG206.311178E-446.023
CGCATCG206.311178E-446.021
CCGTGTA253.416931E-546.033
ATGCTCG206.311178E-446.041
ATTCGGA253.416931E-546.040
ATCCGTA253.416931E-546.041
GTTACGT206.311178E-446.038
GTAGCGA206.311178E-446.035
CCGTAGA253.416931E-546.031
TCGGACG301.8612318E-646.01
TACGATG301.8612318E-646.01
GTAGACC253.416931E-546.043
GCTTACG301.8612318E-646.036
GCGATAT253.416931E-546.09
CTCGATT253.416931E-546.027
CGTAACA206.311178E-446.014
Produced by FastQC (version 0.11.5)