FastQCFastQC Report
Thu 26 May 2016
SRR1544711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544711_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1512648
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79740.5271550287971821No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42110.27838598272697945No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC206.31209E-446.017
AGATCGA253.4176723E-546.044
CGAACAG253.4176723E-546.033
CGTATGA206.31209E-446.015
AAGCGTA206.31209E-446.027
TATTCGG405.6134013E-946.02
GCGATCT1100.046.09
CTCGCGA206.31209E-446.026
TTACGTA206.31209E-446.020
TGAATCG253.4176723E-546.01
TCGACTA253.4176723E-546.032
TCGAATA253.4176723E-546.012
CCGTTCG253.4176723E-546.01
GCGTAAT253.4176723E-546.09
TACGTAG253.4176723E-546.01
TCGTTAC301.8617975E-645.99999621
TACGCGG700.045.9999962
TATTACG351.0198528E-745.9999961
ACTCGAT351.0198528E-745.99999618
TTACGAG700.045.9999961
Produced by FastQC (version 0.11.5)