##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544711_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1512648
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69437502974915	33.0	31.0	34.0	31.0	34.0
2	33.1181140622273	34.0	33.0	34.0	31.0	34.0
3	33.16430921139617	34.0	33.0	34.0	31.0	34.0
4	36.49036127373983	37.0	37.0	37.0	35.0	37.0
5	36.49163519867147	37.0	37.0	37.0	35.0	37.0
6	36.55249668131647	37.0	37.0	37.0	35.0	37.0
7	36.626316234841156	37.0	37.0	37.0	35.0	37.0
8	36.61280549076851	37.0	37.0	37.0	35.0	37.0
9	38.30686848493503	39.0	39.0	39.0	37.0	39.0
10	38.234888090289346	39.0	39.0	39.0	37.0	39.0
11	38.105885837286664	39.0	39.0	39.0	35.0	39.0
12	37.54879786969606	39.0	35.0	39.0	35.0	39.0
13	37.44916993246281	39.0	35.0	39.0	35.0	39.0
14	38.69415290272423	41.0	36.0	41.0	35.0	41.0
15	38.707850074835655	41.0	36.0	41.0	35.0	41.0
16	38.75095527842565	41.0	36.0	41.0	35.0	41.0
17	38.71716023820479	40.0	36.0	41.0	35.0	41.0
18	38.7218334999286	40.0	36.0	41.0	35.0	41.0
19	38.70190883801122	40.0	36.0	41.0	35.0	41.0
20	38.568034334491564	40.0	35.0	41.0	35.0	41.0
21	38.50224969721971	40.0	35.0	41.0	35.0	41.0
22	38.42513922604598	40.0	35.0	41.0	35.0	41.0
23	38.355304076030905	40.0	35.0	41.0	35.0	41.0
24	38.32709724932701	40.0	35.0	41.0	35.0	41.0
25	38.31301730475299	40.0	35.0	41.0	35.0	41.0
26	38.26088620749837	40.0	35.0	41.0	35.0	41.0
27	38.231946890486086	40.0	35.0	41.0	35.0	41.0
28	38.18501991210116	40.0	35.0	41.0	35.0	41.0
29	38.15220130526071	40.0	36.0	41.0	35.0	41.0
30	38.09021662673669	40.0	36.0	41.0	35.0	41.0
31	37.984658691248725	40.0	35.0	41.0	35.0	41.0
32	37.85774350675108	40.0	35.0	41.0	35.0	41.0
33	37.72183548320561	40.0	35.0	41.0	35.0	41.0
34	37.58031875228077	40.0	35.0	41.0	34.0	41.0
35	37.44662604915354	40.0	35.0	41.0	34.0	41.0
36	37.344087983456824	40.0	35.0	41.0	34.0	41.0
37	37.31763437362823	40.0	35.0	41.0	34.0	41.0
38	37.204926063433135	40.0	35.0	41.0	33.0	41.0
39	37.19755356170107	40.0	35.0	41.0	34.0	41.0
40	37.10498741280192	40.0	35.0	41.0	33.0	41.0
41	37.058936381762315	40.0	35.0	41.0	33.0	41.0
42	37.035918468804375	40.0	35.0	41.0	33.0	41.0
43	36.996443323231844	40.0	35.0	41.0	33.0	41.0
44	36.895112412140826	39.0	35.0	41.0	33.0	41.0
45	36.827784124264205	39.0	35.0	41.0	33.0	41.0
46	36.767433004902664	39.0	35.0	41.0	33.0	41.0
47	36.704198200771096	39.0	35.0	41.0	33.0	41.0
48	36.647015035884095	39.0	35.0	41.0	33.0	41.0
49	36.60521615075021	39.0	35.0	41.0	33.0	41.0
50	36.496555047836644	39.0	35.0	41.0	33.0	41.0
51	36.42872697415393	38.0	35.0	41.0	33.0	41.0
52	36.212426817078395	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	7.0
14	9.0
15	16.0
16	34.0
17	86.0
18	194.0
19	313.0
20	675.0
21	1116.0
22	1770.0
23	2899.0
24	5013.0
25	8162.0
26	11544.0
27	13044.0
28	13053.0
29	12492.0
30	13343.0
31	15603.0
32	19753.0
33	27208.0
34	68523.0
35	265161.0
36	63598.0
37	82726.0
38	160687.0
39	722822.0
40	2791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.717922477668296	23.232371311765856	29.340732278758843	14.708973931807003
2	30.81397654973265	25.65692745437141	30.497709976147792	13.031386019748151
3	28.920211443772775	25.570192139876564	31.802507919886185	13.707088496464479
4	25.560275754835228	27.858629370481435	31.838867998371068	14.742226876312268
5	23.1515197190622	33.4849879152321	29.115630338320614	14.24786202738509
6	22.482692602641198	41.46800841967199	26.02297428086376	10.026324696823055
7	87.95912862741365	4.065453430011477	6.189344778163855	1.7860731644110197
8	88.09147931309862	3.5345301748985882	6.376962783145848	1.9970277288569447
9	83.00582819003496	5.845047889528826	7.904747171846986	3.244376748589229
10	41.487444534353	32.858669036021595	13.561582073291339	12.092304356334058
11	38.23738239167341	21.892006600345884	24.565464007488856	15.305147000491854
12	36.93245222946779	20.735425558358585	27.217568132176158	15.114554079997461
13	21.92869722499881	35.15133725757744	27.062938634764993	15.857026882658754
14	15.602638551731799	37.37650795161862	29.622820378567916	17.398033118081667
15	15.489657871494227	24.971639138781793	43.7522807685595	15.786422221164475
16	17.649777079664272	21.451190230641895	42.5390441133694	18.359988576324433
17	17.498519153167162	21.515448405709723	31.195823483057527	29.79020895806559
18	19.3741042198846	24.276302219683625	36.392207572416055	19.95738598801572
19	25.801442239040412	24.837106848387727	28.912476663440533	20.448974249131325
20	27.74842527805544	24.032755803068525	29.446176506364996	18.77264241251104
21	21.747161269508837	28.20147185597707	31.073323073180276	18.97804380133382
22	20.80365028744295	23.750998249427493	27.861207630592176	27.58414383253738
23	16.94234217081568	30.40535537679619	28.192745437140697	24.459557015247434
24	17.342369143382992	24.710375447559514	40.15117859541678	17.796076813640717
25	17.521723494163876	26.570953718247736	36.08770844241357	19.81961434517482
26	17.268789566376313	33.231128458174005	28.560378885239658	20.939703090210017
27	16.402031404530334	35.75094800640995	28.366546612298432	19.48047397676128
28	15.413896689778456	29.844947403493745	36.130481116558514	18.610674790169295
29	17.30567851873007	24.239214939629047	35.85440895700784	22.600697584633043
30	18.23702540181192	32.51278552577996	30.858732500885864	18.39145657152226
31	25.093742893257385	29.347607639054164	27.753978453678585	17.80467101400987
32	25.98112713598934	26.227185703481577	29.24421279768988	18.547474362839207
33	25.5257667348914	28.694712847932895	26.733251886757525	19.046268530418182
34	18.90988518148307	27.816782225607017	28.28179457481185	24.991538018098066
35	19.51002480418445	26.698346211412037	32.614990400939284	21.17663858346423
36	26.506100560077428	27.754242890613018	27.467989909086583	18.27166664022297
37	18.688551467360547	34.22038702989724	29.506137581248247	17.584923921493964
38	19.235539266240394	33.24395364949414	26.642616127479755	20.877890956785716
39	19.307201675472417	32.716666402229734	28.504979347475423	19.47115257482243
40	23.65137163437892	27.1985286728968	28.777679936112037	20.372419756612246
41	17.242279763699155	26.4336448400421	30.313661869780677	26.01041352647807
42	18.588990961545584	26.27465213321275	28.181639085894407	26.954717819347263
43	19.246182852851422	26.94797467751916	29.161840692613218	24.6440017770162
44	17.358301468682733	29.32658490276654	31.258164490350698	22.05694913820003
45	18.017079981595188	35.42688054325924	26.749184212057266	19.806855263088305
46	19.735787836958764	33.93016749435427	27.056393820637716	19.277650848049248
47	18.626408787768206	29.234428631115765	29.397189564260817	22.741973016855212
48	19.537922900767395	26.360329699969853	33.28057816491345	20.8211692343493
49	20.17508369428975	26.430471596828873	33.586465588821724	19.807979120059656
50	18.926941363754157	33.12026327341193	29.273102532776957	18.67969283005696
51	17.976290584458514	32.00024063761034	27.633791866977646	22.389676910953508
52	18.73496014935398	27.75179684896949	34.28100919711658	19.23223380455995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4887.0
1	5083.0
2	5279.0
3	9788.0
4	14297.0
5	9828.0
6	5359.0
7	5499.5
8	5640.0
9	5774.0
10	5908.0
11	5801.0
12	5694.0
13	5762.0
14	5456.0
15	5082.0
16	5307.5
17	5533.0
18	5315.0
19	5097.0
20	5691.5
21	6286.0
22	7424.5
23	8563.0
24	9566.5
25	10570.0
26	13306.5
27	16043.0
28	19729.5
29	23416.0
30	28351.5
31	33287.0
32	36800.0
33	40313.0
34	50106.0
35	59899.0
36	59071.0
37	58243.0
38	68546.5
39	91328.5
40	103807.0
41	118320.0
42	132833.0
43	142250.5
44	151668.0
45	146896.0
46	142124.0
47	134664.0
48	127204.0
49	113220.5
50	99237.0
51	87009.0
52	74781.0
53	67498.5
54	60216.0
55	54999.5
56	49783.0
57	44367.0
58	38951.0
59	36571.5
60	34192.0
61	31511.0
62	28830.0
63	24775.5
64	17556.5
65	14392.0
66	12072.5
67	9753.0
68	8404.5
69	7056.0
70	5993.0
71	4930.0
72	4163.5
73	3397.0
74	2770.5
75	2144.0
76	1763.5
77	1383.0
78	1040.0
79	697.0
80	483.5
81	270.0
82	181.5
83	93.0
84	83.0
85	73.0
86	47.5
87	22.0
88	14.0
89	5.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1512648.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.12201864723345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1444471507666	12.276021968479409
2	8.787284084062074	2.833375157235729
3	2.9992853708985385	1.4506360403400218
4	1.5384945642624968	0.9921455221482909
5	0.9630613515297439	0.776324653389619
6	0.7100356135136022	0.6868324440759682
7	0.4919147873905973	0.5551461562612761
8	0.41245836900151434	0.5319729212999926
9	0.32656357537490477	0.47383776465312966
>10	4.930881368746727	19.07456867899189
>50	1.1354996178821117	12.924740782805513
>100	1.5497589247609627	45.54551567308949
>500	0.007839568575734368	0.7701122202304098
>1k	0.0020630443620353604	0.5783325250654905
>5k	4.126088724070721E-4	0.5304374919337728
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7974	0.5271550287971821	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4211	0.27838598272697945	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.610923360887661E-5	0.0	0.0	0.07814111412569216	0.0
2	6.610923360887661E-5	0.0	0.0	0.22966347755723737	0.0
3	6.610923360887661E-5	0.0	0.0	0.3339838481920447	0.0
4	6.610923360887661E-5	0.0	0.0	0.49158826111560655	0.0
5	6.610923360887661E-5	0.0	0.0	0.8850704195556401	0.0
6	6.610923360887661E-5	0.0	0.0	1.1783309798446169	0.0
7	6.610923360887661E-5	0.0	0.0	1.3852528810404008	0.0
8	6.610923360887661E-5	0.0	0.0	1.8366467281218102	0.0
9	6.610923360887661E-5	0.0	0.0	1.9995398797340822	0.0
10	6.610923360887661E-5	0.0	0.0	2.312633210105722	0.0
11	6.610923360887661E-5	0.0	0.0	2.7365256160058387	0.0
12	6.610923360887661E-5	0.0	0.0	3.0748065643824605	0.0
13	6.610923360887661E-5	0.0	0.0	3.199224142034366	0.0
14	6.610923360887661E-5	0.0	0.0	3.246029479429451	0.0
15	6.610923360887661E-5	0.0	0.0	3.3239722658543163	0.0
16	6.610923360887661E-5	0.0	0.0	3.491955828454472	0.0
17	6.610923360887661E-5	0.0	0.0	3.6847303536579563	0.0
18	6.610923360887661E-5	0.0	0.0	3.936011550605296	0.0
19	6.610923360887661E-5	0.0	0.0	4.073650974978977	0.0
20	6.610923360887661E-5	0.0	0.0	4.23356921107885	0.0
21	6.610923360887661E-5	0.0	0.0	4.430178071831649	0.0
22	6.610923360887661E-5	0.0	0.0	4.628373554191061	0.0
23	6.610923360887661E-5	0.0	0.0	4.863656316605053	0.0
24	6.610923360887661E-5	0.0	0.0	5.043076776619544	0.0
25	6.610923360887661E-5	0.0	0.0	5.193276955378912	0.0
26	6.610923360887661E-5	0.0	0.0	5.328866993510718	0.0
27	6.610923360887661E-5	0.0	0.0	5.477678878364299	0.0
28	6.610923360887661E-5	0.0	0.0	5.627548510955622	0.0
29	6.610923360887661E-5	0.0	0.0	5.796655930527129	0.0
30	6.610923360887661E-5	0.0	0.0	5.98903380032896	0.0
31	6.610923360887661E-5	0.0	0.0	6.178965628487262	0.0
32	6.610923360887661E-5	0.0	0.0	6.344635367911107	0.0
33	6.610923360887661E-5	0.0	0.0	6.519758727741021	0.0
34	6.610923360887661E-5	0.0	0.0	6.698319767718597	0.0
35	6.610923360887661E-5	0.0	0.0	6.915224163189321	0.0
36	6.610923360887661E-5	0.0	0.0	7.098941723388389	0.0
37	6.610923360887661E-5	0.0	0.0	7.288146349976994	0.0
38	6.610923360887661E-5	0.0	0.0	7.467897356159529	0.0
39	6.610923360887661E-5	0.0	0.0	7.657432528916178	0.0
40	6.610923360887661E-5	0.0	0.0	7.867064908689927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	6.31209E-4	46.0	17
AGATCGA	25	3.4176723E-5	46.0	44
CGAACAG	25	3.4176723E-5	46.0	33
CGTATGA	20	6.31209E-4	46.0	15
AAGCGTA	20	6.31209E-4	46.0	27
TATTCGG	40	5.6134013E-9	46.0	2
GCGATCT	110	0.0	46.0	9
CTCGCGA	20	6.31209E-4	46.0	26
TTACGTA	20	6.31209E-4	46.0	20
TGAATCG	25	3.4176723E-5	46.0	1
TCGACTA	25	3.4176723E-5	46.0	32
TCGAATA	25	3.4176723E-5	46.0	12
CCGTTCG	25	3.4176723E-5	46.0	1
GCGTAAT	25	3.4176723E-5	46.0	9
TACGTAG	25	3.4176723E-5	46.0	1
TCGTTAC	30	1.8617975E-6	45.999996	21
TACGCGG	70	0.0	45.999996	2
TATTACG	35	1.0198528E-7	45.999996	1
ACTCGAT	35	1.0198528E-7	45.999996	18
TTACGAG	70	0.0	45.999996	1
>>END_MODULE