##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544710_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	940031
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7194730812069	33.0	31.0	34.0	31.0	34.0
2	33.118224824500466	34.0	33.0	34.0	31.0	34.0
3	33.15736076788957	34.0	33.0	34.0	31.0	34.0
4	36.48163411632169	37.0	37.0	37.0	35.0	37.0
5	36.484276582368025	37.0	37.0	37.0	35.0	37.0
6	36.544779906194584	37.0	37.0	37.0	35.0	37.0
7	36.64281177961152	37.0	37.0	37.0	35.0	37.0
8	36.64784884753801	37.0	37.0	37.0	35.0	37.0
9	38.39513164991367	39.0	39.0	39.0	37.0	39.0
10	38.29318075680483	39.0	39.0	39.0	37.0	39.0
11	38.04753141119814	39.0	39.0	39.0	35.0	39.0
12	37.025450224513875	39.0	35.0	39.0	35.0	39.0
13	36.88124859712073	39.0	35.0	39.0	35.0	39.0
14	37.88319321384082	40.0	35.0	41.0	35.0	41.0
15	37.93678719106072	40.0	35.0	41.0	35.0	41.0
16	38.031781930595905	40.0	35.0	41.0	35.0	41.0
17	38.00064359579631	40.0	35.0	41.0	35.0	41.0
18	38.069772167088104	39.0	36.0	41.0	35.0	41.0
19	38.01559203898595	39.0	36.0	41.0	35.0	41.0
20	37.84218073659273	39.0	35.0	41.0	35.0	41.0
21	37.742459557184816	39.0	35.0	41.0	35.0	41.0
22	37.68669011979392	39.0	35.0	41.0	35.0	41.0
23	37.63117492933743	39.0	35.0	41.0	35.0	41.0
24	37.57825646175498	39.0	35.0	41.0	35.0	41.0
25	37.567867442669446	39.0	35.0	41.0	35.0	41.0
26	37.516054257785115	38.0	35.0	41.0	35.0	41.0
27	37.498349522515745	38.0	35.0	41.0	35.0	41.0
28	37.507835379897045	38.0	35.0	41.0	35.0	41.0
29	37.48308938747765	38.0	35.0	41.0	35.0	41.0
30	37.438075978345395	38.0	35.0	41.0	35.0	41.0
31	37.25417353257499	38.0	35.0	41.0	35.0	41.0
32	37.1034295677483	38.0	35.0	41.0	34.0	41.0
33	36.97945386907453	38.0	35.0	41.0	34.0	41.0
34	36.82537597164349	38.0	35.0	41.0	34.0	41.0
35	36.69002405239827	38.0	35.0	41.0	34.0	41.0
36	36.60458538069489	38.0	35.0	41.0	33.0	41.0
37	36.54319804346878	38.0	35.0	41.0	33.0	41.0
38	36.43640050168558	37.0	35.0	41.0	33.0	41.0
39	36.45444458746573	37.0	35.0	41.0	33.0	41.0
40	36.310234449714955	37.0	35.0	41.0	32.0	41.0
41	36.327659406977006	37.0	35.0	41.0	33.0	41.0
42	36.33029017128158	37.0	35.0	41.0	33.0	41.0
43	36.26414979931513	37.0	35.0	41.0	33.0	41.0
44	36.166120053487596	36.0	35.0	41.0	33.0	41.0
45	36.09022893925839	36.0	35.0	41.0	33.0	41.0
46	36.04668782199736	36.0	35.0	40.0	33.0	41.0
47	35.99501824939816	36.0	35.0	40.0	33.0	41.0
48	35.95301963445886	36.0	35.0	40.0	33.0	41.0
49	35.9650341318531	36.0	35.0	40.0	33.0	41.0
50	35.81850917682502	36.0	35.0	40.0	33.0	41.0
51	35.72843129641469	35.0	35.0	40.0	32.0	41.0
52	35.47451201077411	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	1.0
14	2.0
15	8.0
16	19.0
17	53.0
18	118.0
19	236.0
20	491.0
21	726.0
22	1299.0
23	2143.0
24	3570.0
25	5808.0
26	7771.0
27	8558.0
28	8149.0
29	8044.0
30	8543.0
31	10180.0
32	13081.0
33	19122.0
34	54044.0
35	270095.0
36	37804.0
37	43742.0
38	80561.0
39	354061.0
40	1798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.69583662666444	22.820098486113753	30.07443371548385	14.409631171737953
2	29.73976390140325	24.877264685951847	31.7705479925662	13.6124234200787
3	27.34473650337063	25.54394482735144	33.34677260643532	13.764546062842609
4	24.4587678491454	27.969822271818696	32.13606785308144	15.435342025954462
5	23.35316601261022	33.918987778062636	29.369350585246657	13.358495624080483
6	20.56081129239355	42.75805797893899	25.916592112387782	10.764538616279676
7	87.90859024861946	4.845584879647586	5.757895218349182	1.4879296533837714
8	89.14780470005776	3.2781897618270035	5.772256446861858	1.8017490912533736
9	85.17144647357374	5.021430144325027	7.054448204367729	2.7526751777335003
10	55.1365859211026	24.66620781655073	11.696529157017162	8.500677105329506
11	50.94268167751914	17.69388456338142	20.480069274311166	10.883364484788267
12	47.514922380219375	19.886790967531923	21.173131524385898	11.425155127862805
13	20.101996636281143	46.93674995824606	20.791016466478233	12.170236938994565
14	12.515119182239735	48.646268048606906	24.951198417924513	13.887414351228841
15	11.152930062944733	24.554083854681387	52.135089161953175	12.15789692042071
16	13.145417544740546	18.986395129522325	50.8856622813503	16.982525044386833
17	12.80085443990677	19.169686957132264	29.23818469816421	38.79127390479676
18	18.15855009036936	22.62393474257764	39.849962394857194	19.36755277219581
19	27.641109708084095	24.26302962349114	27.052512098005277	21.043348570419486
20	31.165036046683564	21.71779441316297	28.227579728753625	18.889589811399837
21	19.32266063565989	31.144292049943033	30.63441524800778	18.8986320663893
22	19.9988085499308	24.898859718456094	24.311113144140993	30.791218587472112
23	15.334813426365725	31.773101099857342	25.66223879850771	27.229846675269222
24	15.50427592281531	23.021793962114014	45.22127461753921	16.252655497531464
25	13.199671074677324	26.06445957633312	40.565470713199886	20.17039863578967
26	13.605189616087129	37.269090061923485	28.760966393661487	20.364753928327893
27	13.616146701544949	42.34722046400597	27.123148066393554	16.913484768055522
28	12.538203527330483	33.28507251356604	38.80701806642547	15.369705892678006
29	12.932552224341537	24.367494263487053	38.283631071741254	24.416322440430154
30	16.748490209365436	35.22756164424365	31.78512198001981	16.23882616637111
31	29.282544937347808	29.897205517690374	24.798863016219677	16.021386528742138
32	30.505270570864152	25.8373394068919	28.45491265713578	15.202477365108171
33	30.859726966451106	27.707384118183338	22.9750933745802	18.45779554078536
34	20.095294729641893	27.291972286020354	25.840211652594437	26.772521331743317
35	17.976960334286847	25.774894657729376	32.23893680102039	24.009208206963386
36	33.43570584374345	24.66344195031866	25.484691462302838	16.41616074363505
37	18.177592015582462	36.21795451426602	30.226343599306833	15.378109870844686
38	19.278194017005823	35.133203053941834	24.448129902098973	21.140473026953366
39	19.2925552455185	34.03100536046152	28.342150418443644	18.33428897557634
40	25.70606714033899	24.6305706939452	26.472531225034068	23.190830940681746
41	16.39839537206752	24.083567456817914	28.818517687182656	30.699519483931915
42	20.388263791300503	22.524150799282154	27.244420662722824	29.843164746694523
43	19.749135932751155	24.46195923325933	27.81759324958432	27.971311584405196
44	15.95745246699311	30.068157326726457	30.88100286054396	23.09338734573647
45	14.85802063974486	42.44902561724028	23.174980399582566	19.517973343432292
46	21.53801310807835	35.63339932406485	25.726385619197668	17.10220194865914
47	19.468719648607333	29.58349245929124	26.43072409314161	24.51706379895982
48	20.272310168494442	25.70734369398456	34.04270710221259	19.97763903530841
49	22.017252622519894	24.384621358231804	34.970229705190576	18.62789631405773
50	20.220822504789734	35.08831091740591	27.263037070054075	17.427829507750275
51	17.628354809575427	35.0550141431506	25.20650914703877	22.110121900235207
52	18.470986595122927	27.713873265881656	35.32234575242732	18.492794386568104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5386.0
1	4621.5
2	3857.0
3	5631.0
4	7405.0
5	5078.5
6	2752.0
7	2998.0
8	3244.0
9	3416.5
10	3589.0
11	3824.5
12	4060.0
13	4001.0
14	3884.5
15	3827.0
16	3601.5
17	3376.0
18	3289.5
19	3203.0
20	3945.5
21	4688.0
22	4429.5
23	4171.0
24	4285.0
25	4399.0
26	4562.0
27	4725.0
28	7792.0
29	10859.0
30	13343.0
31	15827.0
32	17703.5
33	19580.0
34	21493.0
35	23406.0
36	25695.0
37	27984.0
38	32882.0
39	48316.0
40	58852.0
41	82408.5
42	105965.0
43	109749.5
44	113534.0
45	106677.5
46	99821.0
47	93008.5
48	86196.0
49	80432.0
50	74668.0
51	65836.0
52	57004.0
53	50955.5
54	44907.0
55	37166.0
56	29425.0
57	24875.5
58	20326.0
59	18056.0
60	15786.0
61	13873.5
62	11961.0
63	10510.5
64	6796.5
65	4533.0
66	4085.0
67	3637.0
68	3076.5
69	2516.0
70	2016.5
71	1517.0
72	1268.0
73	1019.0
74	838.5
75	658.0
76	490.5
77	323.0
78	235.5
79	148.0
80	92.0
81	36.0
82	39.5
83	43.0
84	25.5
85	8.0
86	11.5
87	15.0
88	13.0
89	5.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	940031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.559795581866034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15937152789559	12.611836241453389
2	9.844874113305982	3.260582056911033
3	3.243150522707948	1.6111772909179676
4	1.6724539256186948	1.1078198051333985
5	1.0212298791073873	0.8455679020056048
6	0.6896672666024924	0.6852449372664947
7	0.5153977007596419	0.59744163975604
8	0.39429570875796244	0.5223565068671073
9	0.3203706550189913	0.47747453017887176
>10	3.59576347316977	14.201429293486957
>50	1.1012902125270987	12.791226958131801
>100	1.4292242265738548	48.844108731261564
>500	0.009037551568217691	0.8651417377495543
>1k	0.0038732363863790108	1.578592368880226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4724	0.5025366184732206	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4391	0.46711225480861807	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1629	0.17329215738629897	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	1568	0.16680300968797837	Illumina Single End Adapter 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1368	0.145527115595124	TruSeq Adapter, Index 22 (96% over 25bp)
TTAGCGGGGAGGATTCTTACCAAAATGAATTATCATCACAGAACTTATAAAA	1087	0.11563448439466358	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.179568546143691	0.0
2	0.0	0.0	0.0	0.5005154085343994	0.0
3	0.0	0.0	0.0	0.6717863559818772	0.0
4	0.0	0.0	0.0	0.8715670015137799	0.0
5	0.0	0.0	0.0	1.383784151799249	0.0
6	0.0	0.0	0.0	1.7570697136583793	0.0
7	0.0	0.0	0.0	2.0024871520194547	0.0
8	0.0	0.0	0.0	2.5821488865792723	0.0
9	0.0	0.0	0.0	2.7863974698706744	0.0
10	0.0	0.0	0.0	3.221276745128618	0.0
11	1.0637947046427193E-4	0.0	0.0	3.7477487444563	0.0
12	1.0637947046427193E-4	0.0	0.0	4.166671099144603	0.0
13	1.0637947046427193E-4	0.0	0.0	4.319112880319905	0.0
14	1.0637947046427193E-4	0.0	0.0	4.36879209302672	0.0
15	1.0637947046427193E-4	0.0	0.0	4.460278437625993	0.0
16	1.0637947046427193E-4	0.0	0.0	4.670165132852001	0.0
17	1.0637947046427193E-4	0.0	0.0	4.925582241436719	0.0
18	1.0637947046427193E-4	0.0	0.0	5.293761588713564	0.0
19	1.0637947046427193E-4	0.0	0.0	5.479500144144183	0.0
20	1.0637947046427193E-4	0.0	0.0	5.678429753912371	0.0
21	1.0637947046427193E-4	0.0	0.0	5.938208420786123	0.0
22	1.0637947046427193E-4	0.0	0.0	6.186604484320198	0.0
23	1.0637947046427193E-4	0.0	0.0	6.484892519502017	0.0
24	1.0637947046427193E-4	0.0	0.0	6.6906304153799185	0.0
25	2.1275894092854386E-4	0.0	0.0	6.862858778061574	0.0
26	2.1275894092854386E-4	0.0	0.0	7.035299899684159	0.0
27	2.1275894092854386E-4	0.0	0.0	7.195826520614745	0.0
28	2.1275894092854386E-4	0.0	0.0	7.3499703733174755	0.0
29	2.1275894092854386E-4	0.0	0.0	7.542304455916879	0.0
30	2.1275894092854386E-4	0.0	0.0	7.777190326701992	0.0
31	2.1275894092854386E-4	0.0	0.0	8.005587049788783	0.0
32	2.1275894092854386E-4	0.0	0.0	8.192070261512653	0.0
33	2.1275894092854386E-4	0.0	0.0	8.392276424926411	0.0
34	2.1275894092854386E-4	0.0	0.0	8.598546218156635	0.0
35	2.1275894092854386E-4	0.0	0.0	8.866941622137993	0.0
36	2.1275894092854386E-4	0.0	0.0	9.073956071661467	0.0
37	2.1275894092854386E-4	0.0	0.0	9.281715177478189	0.0
38	2.1275894092854386E-4	0.0	0.0	9.486602037592377	0.0
39	2.1275894092854386E-4	0.0	0.0	9.717126350088455	0.0
40	2.1275894092854386E-4	0.0	0.0	9.980628298428456	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	6.3108635E-4	46.000004	1
AGCGTAT	20	6.3108635E-4	46.000004	15
CGGCGAA	20	6.3108635E-4	46.000004	27
TCGCATA	20	6.3108635E-4	46.000004	1
GCCCATA	20	6.3108635E-4	46.000004	17
ATCTCGC	20	6.3108635E-4	46.000004	37
ACACGTA	20	6.3108635E-4	46.000004	37
CGTATGG	55	1.8189894E-12	46.000004	2
GTCGAGC	20	6.3108635E-4	46.000004	16
CTCCGAT	20	6.3108635E-4	46.000004	45
TTAGACT	55	1.8189894E-12	46.000004	44
TAAACGG	20	6.3108635E-4	46.000004	2
CAACCCG	20	6.3108635E-4	46.000004	23
AAGCGTA	20	6.3108635E-4	46.000004	14
CGCGATC	20	6.3108635E-4	46.000004	33
CGACATA	20	6.3108635E-4	46.000004	23
ATACTCG	40	5.6097633E-9	46.000004	44
GACTTAT	20	6.3108635E-4	46.000004	32
AGACGAG	20	6.3108635E-4	46.000004	1
TAACCTA	20	6.3108635E-4	46.000004	44
>>END_MODULE