FastQCFastQC Report
Thu 26 May 2016
SRR1544709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544709_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1191138
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52020.436725215718078No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43000.36099931326177154No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCTT15870.13323393259219335TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT15260.12811277954359612TruSeq Adapter, Index 15 (95% over 23bp)
GCATGTGGGTTTTCTGACTCCTTTCCTACAGGATCCACCCCAGACCCTAGAT13720.11518396692910476No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13240.1111542071531594No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA206.3115463E-446.00000421
TCGCAAG206.3115463E-446.0000041
CGTATTG206.3115463E-446.00000418
AATCACG206.3115463E-446.00000416
CAACGAA405.6115823E-946.00000440
GCAACTA405.6115823E-946.00000413
CGTGAAC700.046.00000435
GGCGTAA206.3115463E-446.00000422
ATTACGT206.3115463E-446.00000423
ACCGAGT206.3115463E-446.00000420
CAACCGA351.0196163E-746.00000426
ACCGAAT206.3115463E-446.00000416
TCGATAG206.3115463E-446.0000041
ATCCGTT206.3115463E-446.00000444
CGTACGA206.3115463E-446.00000430
ACTCGAA206.3115463E-446.00000415
TCATCCG351.0196163E-746.00000442
CCGGTCT206.3115463E-446.00000415
ACGTAGC206.3115463E-446.00000415
TAGGCGA206.3115463E-446.0000041
Produced by FastQC (version 0.11.5)