FastQCFastQC Report
Thu 26 May 2016
SRR1544708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544708_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1462091
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54420.3722066547157461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43790.299502561742053No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT18950.12960889575272674No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT18830.12878815340495223Illumina Single End Adapter 1 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC351.0198346E-746.00000421
CTTCGCG351.0198346E-746.0000041
CGGCGTG351.0198346E-746.0000042
TTAGCGA700.046.0000041
ATTACGA351.0198346E-746.00000433
ACTTCGC351.0198346E-746.00000445
CGCGAGG2100.046.0000042
CGCGACA551.8189894E-1246.00000414
TAACGCG351.0198346E-746.0000041
TAGTTCG351.0198346E-746.0000041
CGCCTAT551.8189894E-1246.00000429
TCGTGTA351.0198346E-746.00000444
ATATCGG351.0198346E-746.0000042
CGGTGAT351.0198346E-746.0000046
ACTCGCG351.0198346E-746.00000444
TGTACCG351.0198346E-746.0000041
TATCGCA351.0198346E-746.00000418
GTTGCGT351.0198346E-746.00000426
TATCCGG700.046.0000042
CCCGATA551.8189894E-1246.00000413
Produced by FastQC (version 0.11.5)