FastQCFastQC Report
Thu 26 May 2016
SRR1544707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544707_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2159142
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118870.5505427618933818No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67010.3103547612894381No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA21750.10073445840986836No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA206.3126936E-446.033
GTCGAAC206.3126936E-446.041
CGAGTGA206.3126936E-446.029
CGTTAGT206.3126936E-446.043
ACGGATC206.3126936E-446.05
TTCGCAC206.3126936E-446.012
GTGCGAC206.3126936E-446.09
CTAGCGA206.3126936E-446.035
TAGCGAG1100.043.9090921
AACGGGA6850.043.6496354
TCTAGCG950.043.578951
AGTACGG2600.043.3461532
GCGTGAT700.042.71428731
CCGCTAT601.8189894E-1242.16666824
CCGTAAG601.8189894E-1242.1666681
CGTTTTT62300.042.049761
TACGGGA8500.041.9411774
TAGTGCG1600.041.68751
GCGATAT1050.041.6190459
CGTAGAT508.731149E-1041.411
Produced by FastQC (version 0.11.5)