FastQCFastQC Report
Thu 26 May 2016
SRR1544706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544706_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1154647
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54680.4735646478967165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48090.4164909275302322No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT18780.1626471120610888TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT17550.15199450568009096TruSeq Adapter, Index 23 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13960.1209027520965282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG750.046.0000041
TACAGCG750.046.0000041
CTAGATC206.3114666E-446.026
CGTGGAC253.4171666E-546.011
ACTATCG253.4171666E-546.043
TCGTTAG501.6370905E-1146.01
CGAACTT301.86141E-646.011
CGTATGG850.046.02
AAATACG206.3114666E-446.010
TCCGATC301.86141E-646.018
CTATCGA206.3114666E-446.044
GCGAAAA253.4171666E-546.01
GTTTACG253.4171666E-546.01
TAGCGTC206.3114666E-446.014
CGCATCT253.4171666E-546.046
GGCGTAA253.4171666E-546.021
AACCACG206.3114666E-446.028
GAATCGC253.4171666E-546.09
TTCGTTC253.4171666E-546.012
TTCGTCA453.092282E-1046.020
Produced by FastQC (version 0.11.5)