##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544706_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1154647
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66280343689456	33.0	31.0	34.0	31.0	34.0
2	33.07319639682085	34.0	33.0	34.0	31.0	34.0
3	33.11674217314902	34.0	33.0	34.0	31.0	34.0
4	36.43586654622582	37.0	37.0	37.0	35.0	37.0
5	36.43454579624768	37.0	37.0	37.0	35.0	37.0
6	36.5004291354847	37.0	37.0	37.0	35.0	37.0
7	36.611020511030645	37.0	37.0	37.0	35.0	37.0
8	36.601600315940715	37.0	37.0	37.0	35.0	37.0
9	38.31283154072197	39.0	39.0	39.0	37.0	39.0
10	38.213491222858586	39.0	39.0	39.0	37.0	39.0
11	38.025427684824884	39.0	39.0	39.0	35.0	39.0
12	37.15940802686882	39.0	35.0	39.0	35.0	39.0
13	37.02118309751812	39.0	35.0	39.0	35.0	39.0
14	38.11934296802399	40.0	35.0	41.0	35.0	41.0
15	38.17548220365185	40.0	35.0	41.0	35.0	41.0
16	38.25065842634156	40.0	35.0	41.0	35.0	41.0
17	38.218025076062204	40.0	35.0	41.0	35.0	41.0
18	38.25577427560111	40.0	36.0	41.0	35.0	41.0
19	38.20840828409029	40.0	36.0	41.0	35.0	41.0
20	38.06307901895558	40.0	35.0	41.0	35.0	41.0
21	37.981947729479224	40.0	35.0	41.0	35.0	41.0
22	37.905056696981845	40.0	35.0	41.0	35.0	41.0
23	37.838713476932774	40.0	35.0	41.0	35.0	41.0
24	37.788164694491044	40.0	35.0	41.0	35.0	41.0
25	37.77978724233467	40.0	35.0	41.0	35.0	41.0
26	37.71462100538086	39.0	35.0	41.0	35.0	41.0
27	37.691540358222035	40.0	35.0	41.0	35.0	41.0
28	37.67539429799757	39.0	35.0	41.0	35.0	41.0
29	37.65076079529068	39.0	35.0	41.0	35.0	41.0
30	37.58956287073019	39.0	35.0	41.0	35.0	41.0
31	37.461304623837414	39.0	35.0	41.0	34.0	41.0
32	37.341255812382485	39.0	35.0	41.0	34.0	41.0
33	37.212220704682906	39.0	35.0	41.0	34.0	41.0
34	37.07100871521772	39.0	35.0	41.0	34.0	41.0
35	36.959479390670914	39.0	35.0	41.0	34.0	41.0
36	36.88631590434132	39.0	35.0	41.0	33.0	41.0
37	36.85918899888884	39.0	35.0	41.0	33.0	41.0
38	36.75201598410597	39.0	35.0	41.0	33.0	41.0
39	36.77304838621674	39.0	35.0	41.0	33.0	41.0
40	36.67333392803168	39.0	35.0	41.0	33.0	41.0
41	36.64054728414832	38.0	35.0	41.0	33.0	41.0
42	36.644644640309984	38.0	35.0	41.0	33.0	41.0
43	36.584849741955765	38.0	35.0	41.0	33.0	41.0
44	36.478793085679	38.0	35.0	41.0	33.0	41.0
45	36.41786277537637	38.0	35.0	41.0	33.0	41.0
46	36.371716204173225	38.0	35.0	41.0	33.0	41.0
47	36.30003542208138	38.0	35.0	41.0	33.0	41.0
48	36.256956455089735	38.0	35.0	41.0	33.0	41.0
49	36.22397667858662	37.0	35.0	40.0	33.0	41.0
50	36.109526980973406	37.0	35.0	40.0	32.0	41.0
51	36.05103637735169	37.0	35.0	40.0	32.0	41.0
52	35.836314475333154	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	3.0
14	1.0
15	14.0
16	34.0
17	75.0
18	173.0
19	348.0
20	612.0
21	948.0
22	1486.0
23	2466.0
24	4120.0
25	6775.0
26	9145.0
27	10312.0
28	10314.0
29	10257.0
30	11603.0
31	13355.0
32	17008.0
33	23646.0
34	62350.0
35	280843.0
36	47226.0
37	52762.0
38	103275.0
39	483251.0
40	2239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.03611146956602	23.42118413679679	30.548037625352165	13.994666768285025
2	29.773775015221105	26.00370502846324	30.753901408828842	13.468618547486807
3	28.62433280474465	26.133701468933797	31.860300161001586	13.381665565319963
4	26.00552376613805	28.256774581322258	31.002202404717632	14.735499247822062
5	22.645968854550354	34.37388223413736	28.743416819166377	14.236732092145912
6	20.64916810072689	43.153795055978144	25.369225399624302	10.827811443670662
7	87.66358895835697	4.41043886140093	6.038122473795021	1.8878497064470787
8	87.67787904008757	3.7218301350975667	6.235758634457111	2.3645321903577456
9	83.43554350377215	5.3433646820197	8.112436095187533	3.1086557190206183
10	49.377428772603224	28.0081271592097	12.601513709384774	10.0129303588023
11	45.763597012766674	19.102028585359854	22.10996087981868	13.024413522054793
12	42.545037574254295	20.230512009298078	24.91272224324837	12.311728173199254
13	20.707107886652803	41.620599196117944	23.75652472140836	13.915768195820888
14	13.123664635165552	44.443886313306145	27.233777942522693	15.198671109005609
15	12.925682048279691	24.33921362979335	49.3333460356282	13.401758286298756
16	15.060360439164524	19.907729375298253	46.742597521147154	18.28931266439007
17	14.958770949043302	20.058684602307025	29.03112379800926	35.951420650640415
18	18.092975602067124	23.656667362406	38.61491867211364	19.635438363413233
19	26.697943180902904	25.249794958978804	27.082129863066374	20.970131997051915
20	29.94057924196746	23.492114906114164	28.544568166721085	18.022737685197292
21	20.130568043739775	29.69963980333383	30.676994787151397	19.492797365774994
22	20.396450170485007	25.095721895956082	25.254385106443788	29.25344282711513
23	15.487850399299527	30.867875636449927	26.923553259134607	26.720720705115937
24	16.34369638512896	23.476958758824125	44.11209659748824	16.067248258558674
25	14.418172826846645	26.79277735966057	38.600108951047375	20.188940862445406
26	14.890091950180445	35.524796756064845	28.303195695307743	21.28191559844697
27	15.39717333522713	38.37640421704642	28.0612169780028	18.16520546972365
28	12.840980836567367	31.513700724117417	38.169674368010305	17.47564407130491
29	14.08785542247977	24.832264752777256	36.75746786680258	24.322411957940393
30	16.406486138187688	35.347339922937486	30.254354794149208	17.991819144725618
31	27.707429196975355	29.30575318690474	27.149769583257914	15.837048032861992
32	29.189440582273196	25.03336517567707	28.62320691951739	17.15398732253234
33	27.860029948547044	28.786806703693856	24.17284243582671	19.18032091193239
34	18.330450778463028	27.986215700556098	26.791391654765484	26.89194186621539
35	18.515009349177713	26.01600315940716	32.586496132584244	22.88249135883088
36	30.12115391110876	26.197184074440067	25.603842559674085	18.07781945477709
37	17.780758967892353	35.98874807625187	30.32667126836167	15.9038216874941
38	18.05616781579132	34.94591853614135	26.177957419020704	20.81995622904663
39	18.775435262898533	34.35976536551864	28.08512038744309	18.779678984139743
40	24.720109262830977	25.82408303143731	27.318046121455303	22.137761584276404
41	15.509502038285294	25.093123699277786	30.023375109449034	29.373999152987885
42	18.753783623912764	25.234465598576882	26.42392003789903	29.587830739611327
43	19.6227071996896	23.069994552447632	29.85241376801741	27.454884479845354
44	16.35079812271629	28.410328005009323	32.09041378014233	23.148460092132055
45	15.353350417919936	40.14620918774309	24.879725145434058	19.62071524890291
46	20.456814939977328	34.845974570583046	26.744277688332453	17.952932801107178
47	17.98030047278519	29.064727141715174	28.031770748982154	24.92320163651748
48	19.32486725380138	26.131882731258994	34.21409313842239	20.32915687651724
49	20.487473660781173	25.006690356446605	35.1527349917334	19.35310099103882
50	18.750232755119097	34.32979949716234	28.196842844609648	18.723124903108914
51	16.951587801293382	33.20642586002475	26.52767469191883	23.314311646763038
52	17.940288243939488	27.14535264890482	35.75698893254822	19.157370174607475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5442.0
1	5015.5
2	4589.0
3	7254.5
4	9920.0
5	6727.0
6	3534.0
7	3626.0
8	3718.0
9	3881.5
10	4045.0
11	4059.0
12	4073.0
13	4023.5
14	3993.5
15	4013.0
16	3901.0
17	3789.0
18	3726.0
19	3663.0
20	4070.5
21	4478.0
22	4593.0
23	4708.0
24	6050.0
25	7392.0
26	8410.5
27	9429.0
28	10346.0
29	11263.0
30	16601.5
31	21940.0
32	25816.5
33	29693.0
34	31817.5
35	33942.0
36	37933.5
37	41925.0
38	45114.0
39	63846.0
40	79389.0
41	101936.0
42	124483.0
43	131926.5
44	139370.0
45	127483.5
46	115597.0
47	112427.0
48	109257.0
49	96989.5
50	84722.0
51	77060.5
52	69399.0
53	58599.5
54	47800.0
55	40108.5
56	32417.0
57	28119.5
58	23822.0
59	20966.5
60	18111.0
61	15983.5
62	13856.0
63	11971.0
64	8396.5
65	6707.0
66	5873.0
67	5039.0
68	4430.0
69	3821.0
70	3152.5
71	2484.0
72	2169.0
73	1854.0
74	1448.5
75	1043.0
76	889.5
77	736.0
78	596.5
79	457.0
80	299.5
81	142.0
82	145.5
83	149.0
84	84.5
85	20.0
86	30.0
87	40.0
88	23.0
89	5.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1154647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.985110664684644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.25695108022599	12.782476423843969
2	10.226945907285973	3.4741161599399195
3	3.691235506591913	1.8808813070663077
4	1.7619074299339597	1.1970477071343364
5	1.005830984139879	0.8542075287792255
6	0.6512449748442801	0.663688078052992
7	0.4855489356868943	0.5772971684033222
8	0.3756128851200273	0.5103861136676114
9	0.31808557958154765	0.4862446893029646
>10	3.8027897572120573	15.355197204200778
>50	0.9572271801478751	11.460141487859925
>100	1.4537951921429446	48.28118713640296
>500	0.009746686185856017	1.0517560359767057
>1k	0.0025649174173305307	0.9489416773939444
>5k	5.129834834661062E-4	0.4764312819750333
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5468	0.4735646478967165	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4809	0.4164909275302322	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT	1878	0.1626471120610888	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	1755	0.15199450568009096	TruSeq Adapter, Index 23 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1396	0.1209027520965282	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16896939064493305	0.0
2	0.0	0.0	0.0	0.5250955486828442	0.0
3	0.0	0.0	0.0	0.7098273325094163	0.0
4	0.0	0.0	0.0	0.9450507384507992	0.0
5	0.0	0.0	0.0	1.5952927604713822	0.0
6	0.0	0.0	0.0	2.0700699001513017	0.0
7	0.0	0.0	0.0	2.392246288259529	0.0
8	8.660655594307177E-5	0.0	0.0	3.0498498675352725	0.0
9	1.7321311188614355E-4	0.0	0.0	3.2667126836167246	0.0
10	1.7321311188614355E-4	0.0	0.0	3.70528828291244	0.0
11	1.7321311188614355E-4	0.0	0.0	4.3018342402483185	0.0
12	1.7321311188614355E-4	0.0	0.0	4.753660642603323	0.0
13	1.7321311188614355E-4	0.0	0.0	4.936833508422921	0.0
14	1.7321311188614355E-4	0.0	0.0	5.006032146621435	0.0
15	1.7321311188614355E-4	0.0	0.0	5.128060783945223	0.0
16	1.7321311188614355E-4	0.0	0.0	5.392817025463193	0.0
17	1.7321311188614355E-4	0.0	0.0	5.697758708938749	0.0
18	1.7321311188614355E-4	0.0	0.0	6.129752209982791	0.0
19	1.7321311188614355E-4	0.0	0.0	6.326435698529507	0.0
20	1.7321311188614355E-4	0.0	0.0	6.528921826324409	0.0
21	1.7321311188614355E-4	0.0	0.0	6.808314575796759	0.0
22	1.7321311188614355E-4	0.0	0.0	7.0787868500069715	0.0
23	1.7321311188614355E-4	0.0	0.0	7.423307729548512	0.0
24	2.598196678292153E-4	0.0	0.0	7.6807024138113205	0.0
25	2.598196678292153E-4	0.0	0.0	7.884401033389426	0.0
26	2.598196678292153E-4	0.0	0.0	8.102389734698138	0.0
27	2.598196678292153E-4	0.0	0.0	8.29838037079731	0.0
28	2.598196678292153E-4	0.0	0.0	8.512644990200469	0.0
29	2.598196678292153E-4	0.0	0.0	8.72327213425402	0.0
30	2.598196678292153E-4	0.0	0.0	9.00777467052701	0.0
31	2.598196678292153E-4	0.0	0.0	9.26664166624085	0.0
32	2.598196678292153E-4	0.0	0.0	9.494590121483016	0.0
33	2.598196678292153E-4	0.0	0.0	9.709807413001549	0.0
34	2.598196678292153E-4	0.0	0.0	9.943818327159729	0.0
35	2.598196678292153E-4	0.0	0.0	10.25646799411422	0.0
36	2.598196678292153E-4	0.0	0.0	10.505288629338663	0.0
37	2.598196678292153E-4	0.0	0.0	10.748999477762467	0.0
38	2.598196678292153E-4	0.0	0.0	10.983270211588477	0.0
39	2.598196678292153E-4	0.0	0.0	11.22074538798438	0.0
40	2.598196678292153E-4	0.0	0.0	11.492170334309966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTACG	75	0.0	46.000004	1
TACAGCG	75	0.0	46.000004	1
CTAGATC	20	6.3114666E-4	46.0	26
CGTGGAC	25	3.4171666E-5	46.0	11
ACTATCG	25	3.4171666E-5	46.0	43
TCGTTAG	50	1.6370905E-11	46.0	1
CGAACTT	30	1.86141E-6	46.0	11
CGTATGG	85	0.0	46.0	2
AAATACG	20	6.3114666E-4	46.0	10
TCCGATC	30	1.86141E-6	46.0	18
CTATCGA	20	6.3114666E-4	46.0	44
GCGAAAA	25	3.4171666E-5	46.0	1
GTTTACG	25	3.4171666E-5	46.0	1
TAGCGTC	20	6.3114666E-4	46.0	14
CGCATCT	25	3.4171666E-5	46.0	46
GGCGTAA	25	3.4171666E-5	46.0	21
AACCACG	20	6.3114666E-4	46.0	28
GAATCGC	25	3.4171666E-5	46.0	9
TTCGTTC	25	3.4171666E-5	46.0	12
TTCGTCA	45	3.092282E-10	46.0	20
>>END_MODULE