FastQCFastQC Report
Thu 26 May 2016
SRR1544705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544705_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1103015
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94380.8556547281768606No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84470.7658100751123058No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT28780.26092120234085664TruSeq Adapter, Index 8 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23970.21731345448611308No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT22020.19963463778824406TruSeq Adapter, Index 13 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT16680.1512218782156181TruSeq Adapter, Index 13 (95% over 24bp)
CAGAGAGGGATGACAAACATACGAAAAACACACCCATTTATTATCATTGGCC11780.10679818497481902No Hit
AAAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT11040.10008930068947386TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGT351.0195254E-746.00000427
AATCACG351.0195254E-746.0000041
CGAGTCT351.0195254E-746.00000414
TCGGGCA750.046.0000045
TAACGTA351.0195254E-746.00000416
ATTCCGA351.0195254E-746.00000431
ACCTAGT351.0195254E-746.00000437
CTAAAGC351.0195254E-746.00000431
GTCGTGG351.0195254E-746.0000041
ACCACGG351.0195254E-746.0000042
GAGCACG750.046.0000041
TTTGACG351.0195254E-746.0000041
CGCGTGG351.0195254E-746.0000042
AGCTAAC351.0195254E-746.00000432
TAGCTAA351.0195254E-746.00000431
CGATATC351.0195254E-746.00000412
TAATCAT253.417066E-546.021
GCATAAC501.6370905E-1146.09
CCACAAT206.3113426E-446.021
CTTCGCT206.3113426E-446.02
Produced by FastQC (version 0.11.5)