FastQCFastQC Report
Thu 26 May 2016
SRR1544704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544704_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1179191
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96070.8147111027814833No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67860.5754792904626986No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT24860.2108225045815309Illumina Single End Adapter 1 (95% over 23bp)
TTGTAAGGGATAGTGAAATTATTGAATAGTAGTTTTTCTGAACTGTTGGATT24740.20980485773721136No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24610.20870240698919854No Hit
AGCAATGGGACTGTGAATATGCCAAGGACTGACTGGGCCTAGCTCGGAACAC24390.20683672110794604No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT23220.19691466437583055Illumina Single End Adapter 1 (95% over 24bp)
GCAATGGGGGAATTCAGTCTGTCCATCCTCCACCCTGCCTCTGCTGTATGGG12220.10363037031320625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT351.0195981E-746.00000438
CTCACCG351.0195981E-746.00000420
TCACCGT351.0195981E-746.00000421
GATCGCG351.0195981E-746.0000041
ACGTTGG351.0195981E-746.0000042
CTATCCG351.0195981E-746.0000041
GACACGG351.0195981E-746.0000042
TCGGAAA351.0195981E-746.00000410
TACGATG351.0195981E-746.0000041
ATCTTCG351.0195981E-746.00000427
GCGATAC351.0195981E-746.0000049
TTACGAG700.046.0000041
GCTCGGA2100.046.00000442
ATCGCTA351.0195981E-746.00000430
TGATCCG453.092282E-1046.01
TTTAGCG405.6115823E-946.01
CTTCGCC253.4172102E-546.033
AACGTAG453.092282E-1046.01
CGGGTAC301.8614446E-646.06
AAGTACG301.8614446E-646.01
Produced by FastQC (version 0.11.5)