##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544704_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1179191
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.717996490814464	33.0	31.0	34.0	31.0	34.0
2	33.10488970828305	34.0	33.0	34.0	31.0	34.0
3	33.13309039841722	34.0	33.0	34.0	31.0	34.0
4	36.449919478693445	37.0	37.0	37.0	35.0	37.0
5	36.45296817903122	37.0	37.0	37.0	35.0	37.0
6	36.52007520410179	37.0	37.0	37.0	35.0	37.0
7	36.6357901306913	37.0	37.0	37.0	35.0	37.0
8	36.63844110072075	37.0	37.0	37.0	35.0	37.0
9	38.387197663482844	39.0	39.0	39.0	37.0	39.0
10	38.30672384711213	39.0	39.0	39.0	37.0	39.0
11	37.996657878155446	39.0	39.0	39.0	35.0	39.0
12	37.162466470656575	39.0	35.0	39.0	35.0	39.0
13	37.03994772687376	39.0	35.0	39.0	35.0	39.0
14	38.10702507057805	40.0	35.0	41.0	35.0	41.0
15	38.165919685614966	40.0	35.0	41.0	35.0	41.0
16	38.23059453472762	40.0	35.0	41.0	35.0	41.0
17	38.18467915715096	40.0	35.0	41.0	35.0	41.0
18	38.22296303143426	40.0	36.0	41.0	35.0	41.0
19	38.1599113290383	40.0	36.0	41.0	35.0	41.0
20	37.987394747755026	40.0	35.0	41.0	35.0	41.0
21	37.912217783208995	40.0	35.0	41.0	35.0	41.0
22	37.84671694407437	40.0	35.0	41.0	35.0	41.0
23	37.799554949113414	39.0	35.0	41.0	35.0	41.0
24	37.73760739354354	39.0	35.0	41.0	35.0	41.0
25	37.7363573840031	39.0	35.0	41.0	35.0	41.0
26	37.672868093464075	39.0	35.0	41.0	35.0	41.0
27	37.644686060188725	39.0	35.0	41.0	35.0	41.0
28	37.68106608683411	39.0	35.0	41.0	35.0	41.0
29	37.646036986374554	39.0	35.0	41.0	35.0	41.0
30	37.57462701123058	39.0	35.0	41.0	35.0	41.0
31	37.43800283414646	39.0	35.0	41.0	35.0	41.0
32	37.269494933390774	39.0	35.0	41.0	35.0	41.0
33	37.079019429422374	39.0	35.0	41.0	34.0	41.0
34	36.95383784306359	39.0	35.0	41.0	34.0	41.0
35	36.81361713242384	38.0	35.0	41.0	34.0	41.0
36	36.73607753112091	38.0	35.0	41.0	34.0	41.0
37	36.67259926508937	38.0	35.0	41.0	33.0	41.0
38	36.55508988789772	38.0	35.0	41.0	33.0	41.0
39	36.58778009669341	38.0	35.0	41.0	33.0	41.0
40	36.460390216682455	38.0	35.0	41.0	32.0	41.0
41	36.43864055950223	38.0	35.0	41.0	33.0	41.0
42	36.439249451530756	38.0	35.0	41.0	33.0	41.0
43	36.37439566618131	38.0	35.0	41.0	33.0	41.0
44	36.26668538006142	38.0	35.0	41.0	33.0	41.0
45	36.19456559624353	38.0	35.0	41.0	33.0	41.0
46	36.11032648654883	37.0	35.0	41.0	33.0	41.0
47	36.07007516169984	37.0	35.0	41.0	33.0	41.0
48	36.02383837732818	37.0	35.0	41.0	33.0	41.0
49	36.0137746980769	37.0	35.0	40.0	32.0	41.0
50	35.871896919158985	37.0	35.0	40.0	32.0	41.0
51	35.81337544129831	37.0	35.0	40.0	32.0	41.0
52	35.55982448984091	36.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	6.0
13	1.0
14	6.0
15	9.0
16	31.0
17	71.0
18	154.0
19	344.0
20	691.0
21	1065.0
22	1671.0
23	2893.0
24	5172.0
25	8315.0
26	10897.0
27	11459.0
28	10684.0
29	10056.0
30	10991.0
31	12900.0
32	16402.0
33	22555.0
34	60475.0
35	309517.0
36	45807.0
37	49164.0
38	99946.0
39	485727.0
40	2181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.842162126407004	24.139940009718526	30.497434257893758	14.52046360598071
2	28.402693032765686	26.661499282134955	31.139484612755698	13.796323072343666
3	27.437709412639684	25.883677877460055	32.90510188765009	13.77351082225017
4	22.778328532018985	28.67805130805781	34.644514756303266	13.899105403619942
5	21.887972347143084	35.80539539396078	30.142445117033628	12.164187141862515
6	21.173414654623382	42.56095916607233	25.707031346066923	10.558594833237365
7	87.3751580532755	5.110537648269025	5.826621811055206	1.68768248740026
8	88.64713180477123	4.0752515919812815	5.562203239339513	1.7154133639079674
9	84.86411446491705	5.645989496188489	7.015572540835199	2.4743234980592628
10	53.31629905587813	28.22273914912851	10.948353574611748	7.5126082203816
11	43.26423793940083	20.19995064412805	25.228398113621964	11.307413302849156
12	42.92451350120549	22.170962973767608	23.37662007257518	11.527903452451724
13	18.447053954787645	45.61754626688976	24.082527766918165	11.852872011404429
14	11.712436746888333	47.55497625066677	26.984517351302717	13.748069651142181
15	11.540708841909412	26.999103622737962	49.446866538160485	12.013320997192142
16	13.06777273571457	22.601512392818464	48.53751427885729	15.79320059260968
17	12.358218473512773	22.352782543286033	29.836387828604526	35.45261115459667
18	16.825688120075544	25.105856472785153	38.747582028695945	19.320873378443356
19	24.950326113411652	25.732896536693378	28.45535625695922	20.86142109293575
20	28.325691088212174	23.08430101654439	30.13735688281203	18.452651012431403
21	18.036772668719486	31.92782170148856	32.53221912311067	17.503186506681274
22	20.25481876981761	24.59694824672169	26.109171457380526	29.039061526080168
23	15.877241261169733	31.67264675527544	26.505884118857757	25.944227864697066
24	14.197021517294484	24.565740410162558	45.07022187245323	16.167016200089723
25	12.432421889244406	27.56627213063872	40.56967870345008	19.4316272766668
26	13.185310946233479	37.6465729470459	29.7456476516527	19.422468455067925
27	13.49196186198843	41.68629170337969	28.679747386131677	16.1419990485002
28	11.846172503012658	34.408251080613745	38.606977156372466	15.138599260001136
29	13.367724143077755	24.56116099936312	38.22731007953758	23.84380477802154
30	15.126472301772994	37.4795940606738	31.232344887299853	16.16158875025335
31	27.395731480311504	31.60531245574296	26.48917774983018	14.509778314115355
32	29.334179110932833	27.139454083350365	29.069082107987594	14.457284697729206
33	27.48604763774486	29.44730751845969	25.480096099783665	17.586548744011786
34	18.619290683188726	29.93815251303648	26.99257372215358	24.449983081621212
35	18.13226186427814	26.48425912341597	34.49246135698119	20.89101765532471
36	31.692151653124895	26.687025257146637	27.216540831807567	14.404282257920897
37	17.727662439757427	36.45185555181476	31.294760560418116	14.525721448009696
38	17.38675074691038	35.25018423648078	26.78183602147574	20.581228995133106
39	18.65863969450242	35.23958374851911	29.677210901372213	16.42456565560626
40	25.059807953079694	26.53802479835752	27.621309864135668	20.780857384427122
41	15.007322817083915	26.467891970003166	30.49107396511676	28.033711247796155
42	19.198840561028703	23.523076414253502	28.404643522550632	28.873439502167166
43	19.752440444338536	24.846356527483675	29.657706003522755	25.74349702465504
44	15.438381059556933	30.373026931175694	32.51593677360156	21.672655235665808
45	14.731370914465936	42.46792928372079	24.468809548241126	18.33189025357215
46	20.964203424212023	36.62689080903772	25.9463479622894	16.462557804460857
47	18.70409458688202	29.65736678790798	28.229438657520284	23.409099967689713
48	20.026102641556793	27.220781026992235	34.557166735499166	18.195949595951802
49	22.330987940036856	25.993668540550257	34.26009866086155	17.41524485855133
50	19.597503712290884	35.8253243113287	27.742749054224465	16.83442292215595
51	17.364871339757514	34.35499422909435	25.118407450531766	23.161726980616372
52	18.07476481757408	27.829164232087933	35.840164994475025	18.25590595586296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10565.0
1	7827.0
2	5089.0
3	8065.5
4	11042.0
5	7504.0
6	3966.0
7	4341.0
8	4716.0
9	5210.0
10	5704.0
11	5825.0
12	5946.0
13	5853.5
14	5528.0
15	5295.0
16	4897.0
17	4499.0
18	4923.5
19	5348.0
20	5192.0
21	5036.0
22	5960.0
23	6884.0
24	7900.0
25	8916.0
26	11677.5
27	14439.0
28	17146.5
29	19854.0
30	21544.0
31	23234.0
32	26189.0
33	29144.0
34	37635.0
35	46126.0
36	44573.5
37	43021.0
38	47483.0
39	67712.0
40	83479.0
41	107100.0
42	130721.0
43	136898.5
44	143076.0
45	136567.0
46	130058.0
47	120537.0
48	111016.0
49	98257.5
50	85499.0
51	73146.5
52	60794.0
53	52401.0
54	44008.0
55	35239.0
56	26470.0
57	22636.5
58	18803.0
59	14941.0
60	11079.0
61	8933.0
62	6787.0
63	5532.5
64	3424.0
65	2570.0
66	2119.0
67	1668.0
68	1220.5
69	773.0
70	626.0
71	479.0
72	530.5
73	582.0
74	411.0
75	240.0
76	191.0
77	142.0
78	110.0
79	78.0
80	53.5
81	29.0
82	19.0
83	9.0
84	5.5
85	2.0
86	6.5
87	11.0
88	9.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1179191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.800278974595225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46930506163963	11.924360739927376
2	10.272549925362023	3.246183092023547
3	3.5545220346419595	1.6848731930606635
4	1.8172588001311503	1.1485278404444144
5	1.101197118405016	0.869961083840981
6	0.6994312904005752	0.6630725707134129
7	0.51992314370255	0.5750451501094197
8	0.40329161934611796	0.5097696075027943
9	0.29526843537876674	0.41987912862390814
>10	3.35883373141862	12.258322135018892
>50	0.9688058984273664	10.868512438490479
>100	1.5136917130946994	50.9163147775532
>500	0.019440921038676125	1.982197825812911
>1k	0.005400255844076701	1.5342386942422603
>5k	0.0010800511688153402	1.3987417226357475
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9607	0.8147111027814833	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6786	0.5754792904626986	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	2486	0.2108225045815309	Illumina Single End Adapter 1 (95% over 23bp)
TTGTAAGGGATAGTGAAATTATTGAATAGTAGTTTTTCTGAACTGTTGGATT	2474	0.20980485773721136	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2461	0.20870240698919854	No Hit
AGCAATGGGACTGTGAATATGCCAAGGACTGACTGGGCCTAGCTCGGAACAC	2439	0.20683672110794604	No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT	2322	0.19691466437583055	Illumina Single End Adapter 1 (95% over 24bp)
GCAATGGGGGAATTCAGTCTGTCCATCCTCCACCCTGCCTCTGCTGTATGGG	1222	0.10363037031320625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2059038781673198	0.0
2	0.0	0.0	0.0	0.6213582023607711	0.0
3	0.0	0.0	0.0	0.8501591345252805	0.0
4	0.0	0.0	0.0	1.1165281960259195	0.0
5	0.0	0.0	0.0	1.7560344337770557	0.0
6	0.0	0.0	0.0	2.165976504230443	0.0
7	0.0	0.0	0.0	2.477291634688528	0.0
8	0.0	0.0	0.0	3.1672561951371745	0.0
9	0.0	0.0	0.0	3.4206502593727395	0.0
10	0.0	0.0	0.0	3.907424666572252	0.0
11	0.0	0.0	0.0	4.457802001541735	0.0
12	0.0	0.0	0.0	4.9170151400409265	0.0
13	0.0	0.0	0.0	5.081025889783758	0.0
14	0.0	0.0	0.0	5.135469995954854	0.0
15	0.0	0.0	0.0	5.233842524239075	0.0
16	0.0	0.0	0.0	5.448990027908965	0.0
17	0.0	0.0	0.0	5.71680075577239	0.0
18	0.0	0.0	0.0	6.080609502616625	0.0
19	0.0	0.0	0.0	6.294145732116341	0.0
20	0.0	0.0	0.0	6.480544712434202	0.0
21	0.0	0.0	0.0	6.7385181874692055	0.0
22	0.0	0.0	0.0	6.985127939409307	0.0
23	8.48039036932948E-5	0.0	0.0	7.271849937796337	0.0
24	1.696078073865896E-4	0.0	0.0	7.49225528349521	0.0
25	2.5441171107988444E-4	0.0	0.0	7.661523875267027	0.0
26	2.5441171107988444E-4	0.0	0.0	7.8359655051641335	0.0
27	2.5441171107988444E-4	0.0	0.0	8.010576742868627	0.0
28	2.5441171107988444E-4	0.0	0.0	8.194940429497851	0.0
29	2.5441171107988444E-4	0.0	0.0	8.379812939549232	0.0
30	2.5441171107988444E-4	0.0	0.0	8.631341317903546	0.0
31	2.5441171107988444E-4	0.0	0.0	8.862601563275161	0.0
32	2.5441171107988444E-4	0.0	0.0	9.054512797333087	0.0
33	2.5441171107988444E-4	0.0	0.0	9.257363734967448	0.0
34	2.5441171107988444E-4	0.0	0.0	9.45173428223248	0.0
35	2.5441171107988444E-4	0.0	0.0	9.749226376388558	0.0
36	2.5441171107988444E-4	0.0	0.0	9.96674838936186	0.0
37	2.5441171107988444E-4	0.0	0.0	10.172821875336565	0.0
38	2.5441171107988444E-4	0.0	0.0	10.387460555584294	0.0
39	2.5441171107988444E-4	0.0	0.0	10.622791388333187	0.0
40	2.5441171107988444E-4	0.0	0.0	10.854899672741736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	35	1.0195981E-7	46.000004	38
CTCACCG	35	1.0195981E-7	46.000004	20
TCACCGT	35	1.0195981E-7	46.000004	21
GATCGCG	35	1.0195981E-7	46.000004	1
ACGTTGG	35	1.0195981E-7	46.000004	2
CTATCCG	35	1.0195981E-7	46.000004	1
GACACGG	35	1.0195981E-7	46.000004	2
TCGGAAA	35	1.0195981E-7	46.000004	10
TACGATG	35	1.0195981E-7	46.000004	1
ATCTTCG	35	1.0195981E-7	46.000004	27
GCGATAC	35	1.0195981E-7	46.000004	9
TTACGAG	70	0.0	46.000004	1
GCTCGGA	210	0.0	46.000004	42
ATCGCTA	35	1.0195981E-7	46.000004	30
TGATCCG	45	3.092282E-10	46.0	1
TTTAGCG	40	5.6115823E-9	46.0	1
CTTCGCC	25	3.4172102E-5	46.0	33
AACGTAG	45	3.092282E-10	46.0	1
CGGGTAC	30	1.8614446E-6	46.0	6
AAGTACG	30	1.8614446E-6	46.0	1
>>END_MODULE