FastQCFastQC Report
Thu 26 May 2016
SRR1544703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544703_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1355004
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58740.43350425533799164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49630.3662719814849255No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT18560.13697376539109848Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT16850.1243538764461212No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15880.1171952259919528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA351.01974365E-746.00000410
TGTTGCG750.046.0000041
TTACGCG351.01974365E-746.0000041
ATACGCG750.046.00000433
AATCGGA351.01974365E-746.00000444
TATCCGT351.01974365E-746.00000413
CGGACCC351.01974365E-746.00000433
GTACCTA253.4174827E-546.010
TCGCACC206.3118566E-446.044
ACGTTTA253.4174827E-546.029
CGACGGT950.046.028
CAACGAA206.3118566E-446.045
ACGCATG206.3118566E-446.01
TAGACGT405.6134013E-946.035
GAGTCGA206.3118566E-446.028
GCAACGC253.4174827E-546.09
GACGTAG501.6370905E-1146.01
GAGTACG453.110472E-1046.01
ATAGCCG253.4174827E-546.016
TATATCG206.3118566E-446.029
Produced by FastQC (version 0.11.5)