##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1544703_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1355004 Sequences flagged as poor quality 0 Sequence length 52 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68682306472896 33.0 31.0 34.0 31.0 34.0 2 33.08605435851112 34.0 33.0 34.0 31.0 34.0 3 33.13087046237502 34.0 33.0 34.0 31.0 34.0 4 36.4532119462378 37.0 37.0 37.0 35.0 37.0 5 36.44918243783782 37.0 37.0 37.0 35.0 37.0 6 36.52185011999965 37.0 37.0 37.0 35.0 37.0 7 36.615004088548815 37.0 37.0 37.0 35.0 37.0 8 36.599697860670524 37.0 37.0 37.0 35.0 37.0 9 38.26967817069175 39.0 39.0 39.0 37.0 39.0 10 38.206993484890084 39.0 39.0 39.0 37.0 39.0 11 38.052407225366125 39.0 39.0 39.0 35.0 39.0 12 37.24051368114043 39.0 35.0 39.0 35.0 39.0 13 37.11544689166969 39.0 35.0 39.0 35.0 39.0 14 38.245092265410285 40.0 35.0 41.0 35.0 41.0 15 38.271998459045136 40.0 35.0 41.0 35.0 41.0 16 38.334330378360505 40.0 35.0 41.0 35.0 41.0 17 38.30999908487355 40.0 35.0 41.0 35.0 41.0 18 38.338873538380696 40.0 36.0 41.0 35.0 41.0 19 38.30483304846332 40.0 36.0 41.0 35.0 41.0 20 38.155063748889305 40.0 35.0 41.0 35.0 41.0 21 38.07716951389073 40.0 35.0 41.0 35.0 41.0 22 37.98826276527597 40.0 35.0 41.0 35.0 41.0 23 37.90651983315178 40.0 35.0 41.0 35.0 41.0 24 37.84725653946409 40.0 35.0 41.0 35.0 41.0 25 37.832393114706676 40.0 35.0 41.0 35.0 41.0 26 37.76810843362824 40.0 35.0 41.0 35.0 41.0 27 37.74726642873379 40.0 35.0 41.0 35.0 41.0 28 37.73501480438434 40.0 35.0 41.0 35.0 41.0 29 37.70741857588612 40.0 35.0 41.0 35.0 41.0 30 37.653634970819276 39.0 35.0 41.0 35.0 41.0 31 37.52397336096425 39.0 35.0 41.0 35.0 41.0 32 37.376208483517395 39.0 35.0 41.0 34.0 41.0 33 37.23689893166367 39.0 35.0 41.0 34.0 41.0 34 37.09749417713896 39.0 35.0 41.0 34.0 41.0 35 36.963684240046526 39.0 35.0 41.0 34.0 41.0 36 36.89215751392616 39.0 35.0 41.0 33.0 41.0 37 36.840125933207574 39.0 35.0 41.0 33.0 41.0 38 36.73098308196876 39.0 35.0 41.0 33.0 41.0 39 36.73761627271949 39.0 35.0 41.0 33.0 41.0 40 36.609869048357055 39.0 35.0 41.0 32.0 41.0 41 36.620880085962845 39.0 35.0 41.0 33.0 41.0 42 36.604903749361625 38.0 35.0 41.0 33.0 41.0 43 36.5606824776901 38.0 35.0 41.0 33.0 41.0 44 36.44905033490676 38.0 35.0 41.0 33.0 41.0 45 36.38255385223955 38.0 35.0 41.0 33.0 41.0 46 36.32071861042477 38.0 35.0 41.0 33.0 41.0 47 36.261775610994505 38.0 35.0 41.0 33.0 41.0 48 36.19893225407453 38.0 35.0 41.0 32.0 41.0 49 36.203431871787835 37.0 35.0 41.0 32.0 41.0 50 36.0596714105641 37.0 35.0 40.0 32.0 41.0 51 35.996886356055036 37.0 35.0 40.0 32.0 41.0 52 35.72779489949845 37.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 1.0 12 2.0 13 2.0 14 8.0 15 25.0 16 36.0 17 93.0 18 213.0 19 409.0 20 715.0 21 1256.0 22 2065.0 23 3401.0 24 5409.0 25 8266.0 26 11275.0 27 12488.0 28 12221.0 29 12256.0 30 13307.0 31 15525.0 32 19719.0 33 27307.0 34 71072.0 35 310005.0 36 56783.0 37 67412.0 38 127384.0 39 574283.0 40 2064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.7700110110376 23.77350915569253 28.5080339246231 13.948445908646764 2 31.87311624172327 25.740883421746357 29.354009287057455 13.031991049472916 3 29.460503437628226 25.329740724012623 31.604851350992323 13.604904487366825 4 25.831067657364848 28.529214673905024 30.961679817919357 14.678037850810771 5 22.850486050225683 33.432004628768624 28.833346617426958 14.884162703578735 6 20.882890382611414 43.28053644122084 25.334316356261677 10.502256819906068 7 87.1469752118813 4.46515287039743 6.5251467892345705 1.8627251284867055 8 87.225646566357 3.7591770946801635 6.669648207680567 2.3455281312822693 9 81.86057015329844 5.9851483833258055 8.512594796768127 3.6416866666076264 10 46.73890261578564 29.493566070653664 13.80911052661099 9.958420786949706 11 44.887911770002155 21.39868221791227 21.19403337554723 12.519372636538343 12 40.5939761063436 21.416468143267473 24.78014825048487 13.20940749990406 13 21.284881815847037 39.40121209974288 24.770849384946462 14.54305669946362 14 14.890066745190419 43.00939333020419 27.903091060985798 14.197448863619591 15 14.070364367928065 24.864133242411093 47.27011875979702 13.795383629863823 16 16.33080049948192 21.474180150021695 45.78052906116882 16.41449028932756 17 15.979731425147085 20.37720921857057 30.199837048451517 33.44322230783083 18 19.127249808856654 23.83432078429289 37.862102252096676 19.176327154753785 19 28.0296589530363 23.16059583587945 29.093050647820967 19.71669456326328 20 28.603310396131672 24.242732862781217 28.305377696302003 18.848579044785108 21 21.01565751835419 28.978069437433398 31.08219606731788 18.924076976894533 22 20.93440314567337 24.55239984531411 26.441545560013108 28.07165144899941 23 16.230505592603418 30.90514861948747 27.15372057942264 25.71062520848647 24 16.730135113992283 24.07527948256979 42.16504157921305 17.029543824224874 25 15.102021839049923 26.192837807120867 38.84962701217118 19.855513341658032 26 14.863867560538566 35.70727466487183 29.521905470389754 19.90695230419984 27 16.1079229286408 37.782471490859066 29.00781104705226 17.101794533447872 28 14.124976752836155 31.335184250378596 37.84446392778176 16.695375069003486 29 15.215600839554716 25.79298658896948 36.57959681299834 22.411815758477466 30 18.08895029092165 32.72447904212829 31.18551679552237 18.001053871427686 31 27.511284099530332 29.32943371384881 25.874314762170442 17.284967424450407 32 28.357997467166147 26.743242086370223 29.036593249909227 15.862167196554402 33 28.513421362593768 28.2520199202364 24.64206747729158 18.59249123987826 34 20.59861077900877 28.318145186287275 27.085971701928557 23.997272332775403 35 18.905774447898306 26.54464488665716 32.39540252279698 22.15417814264755 36 30.16212498265688 25.99416680688765 26.935787643431308 16.907920567024156 37 18.7024540148959 34.71008203665819 30.403969287175535 16.183494661270373 38 19.746362372362 34.60587570221195 24.489448001629516 21.158313923796534 39 20.83580565075823 32.513630956070976 28.515266375597413 18.13529701757338 40 23.939265123940594 25.85136280040502 28.40508219901934 21.80428987663505 41 16.681721972776465 25.874314762170442 29.39873240226597 28.04523086278712 42 19.87898190706448 24.81918872564214 27.895637208451046 27.406192158842334 43 21.034255249431 24.838745863480845 28.286410962624466 25.840587924463694 44 16.958769125404796 30.566404232017025 30.70684662185499 21.767980020723186 45 16.416704304931944 39.393979648768564 24.511440556633044 19.67787548966645 46 20.496249457566176 34.68225924056313 26.236970518168214 18.584520783702484 47 19.84355765739437 29.324562879519174 27.02545527540878 23.806424187677674 48 21.374328046264072 25.53970320382818 33.646247538752654 19.439721211155096 49 22.2920375142804 24.54922642294783 34.01842356184927 19.14031250092251 50 18.955737400037194 35.351334756207365 27.35401519109907 18.338912652656376 51 18.495591156926476 33.39436636349413 25.958742557217544 22.151299922361854 52 19.161345649164137 27.67962308598351 35.21871522150488 17.940316043347472 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5880.0 1 5785.0 2 5690.0 3 8524.5 4 11359.0 5 7933.5 6 4508.0 7 4635.0 8 4762.0 9 5053.5 10 5345.0 11 5487.0 12 5629.0 13 5554.5 14 5398.5 15 5317.0 16 4793.0 17 4269.0 18 4652.0 19 5035.0 20 5038.5 21 5042.0 22 5718.0 23 6394.0 24 7410.5 25 8427.0 26 10338.0 27 12249.0 28 14942.0 29 17635.0 30 20792.5 31 23950.0 32 27280.5 33 30611.0 34 35431.5 35 40252.0 36 43274.0 37 46296.0 38 51388.5 39 71680.5 40 86880.0 41 109781.5 42 132683.0 43 148975.0 44 165267.0 45 156407.5 46 147548.0 47 137249.0 48 126950.0 49 113655.0 50 100360.0 51 87590.0 52 74820.0 53 65749.5 54 56679.0 55 47660.0 56 38641.0 57 34057.0 58 29473.0 59 27571.5 60 25670.0 61 21728.0 62 17786.0 63 15650.0 64 11303.5 65 9093.0 66 7506.0 67 5919.0 68 5280.5 69 4642.0 70 3841.0 71 3040.0 72 2584.0 73 2128.0 74 1836.5 75 1545.0 76 1094.5 77 644.0 78 630.0 79 616.0 80 456.5 81 297.0 82 196.5 83 96.0 84 88.5 85 81.0 86 46.0 87 11.0 88 10.0 89 5.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1355004.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.933379538757123 #Duplication Level Percentage of deduplicated Percentage of total 1 76.18837155889143 12.139382404876658 2 9.777966838993255 3.1159211352612157 3 3.349377614696509 1.6010071426072945 4 1.6318290917151095 1.0400220904272859 5 0.9644659509633018 0.7683601024453302 6 0.6806423417242554 0.6506959656504591 7 0.4905482135143035 0.5471263607587865 8 0.38261366577181144 0.4877063002765949 9 0.31405427007778675 0.45035512928250393 >10 3.5727694860077244 13.949664848644861 >50 1.1496284997715984 12.89521476976394 >100 1.4744323361387262 49.12994187041861 >500 0.020504115926075837 1.9566351684556977 >1k 0.0023300131734177087 0.8318223964863836 >5k 4.6600263468354175E-4 0.43614431464438247 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5874 0.43350425533799164 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4963 0.3662719814849255 No Hit CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT 1856 0.13697376539109848 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT 1685 0.1243538764461212 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1588 0.1171952259919528 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.3800520146066E-5 0.0 0.0 0.1421398018013231 0.0 2 7.3800520146066E-5 0.0 0.0 0.42169617211462107 0.0 3 7.3800520146066E-5 0.0 0.0 0.5726920363334721 0.0 4 7.3800520146066E-5 0.0 0.0 0.7608095621857943 0.0 5 7.3800520146066E-5 0.0 0.0 1.283833848460964 0.0 6 7.3800520146066E-5 0.0 0.0 1.673057791711316 0.0 7 7.3800520146066E-5 0.0 0.0 1.9429462938854793 0.0 8 7.3800520146066E-5 0.0 0.0 2.5056752599992325 0.0 9 7.3800520146066E-5 0.0 0.0 2.702870249829521 0.0 10 7.3800520146066E-5 0.0 0.0 3.1143819501639847 0.0 11 7.3800520146066E-5 0.0 0.0 3.636889632798132 0.0 12 7.3800520146066E-5 0.0 0.0 4.048253732092304 0.0 13 7.3800520146066E-5 0.0 0.0 4.209434068091312 0.0 14 7.3800520146066E-5 0.0 0.0 4.265079660281446 0.0 15 7.3800520146066E-5 0.0 0.0 4.363381953116005 0.0 16 7.3800520146066E-5 0.0 0.0 4.586259523957125 0.0 17 7.3800520146066E-5 0.0 0.0 4.839764310658862 0.0 18 7.3800520146066E-5 0.0 0.0 5.211940333755472 0.0 19 7.3800520146066E-5 0.0 0.0 5.3785081077251435 0.0 20 7.3800520146066E-5 0.0 0.0 5.556293560757016 0.0 21 7.3800520146066E-5 0.0 0.0 5.796145251231731 0.0 22 7.3800520146066E-5 0.0 0.0 6.0282478870911085 0.0 23 7.3800520146066E-5 0.0 0.0 6.302269218393452 0.0 24 7.3800520146066E-5 0.0 0.0 6.4987262030222785 0.0 25 7.3800520146066E-5 0.0 0.0 6.664039368149466 0.0 26 7.3800520146066E-5 0.0 0.0 6.834001966045856 0.0 27 7.3800520146066E-5 0.0 0.0 6.982636213620034 0.0 28 1.47601040292132E-4 0.0 0.0 7.153115415157446 0.0 29 1.47601040292132E-4 0.0 0.0 7.335255098877937 0.0 30 1.47601040292132E-4 0.0 0.0 7.551047819785034 0.0 31 1.47601040292132E-4 0.0 0.0 7.771268571900895 0.0 32 1.47601040292132E-4 0.0 0.0 7.958721893071902 0.0 33 1.47601040292132E-4 0.0 0.0 8.157761895905843 0.0 34 1.47601040292132E-4 0.0 0.0 8.356949499780075 0.0 35 2.2140156043819797E-4 0.0 0.0 8.610011483360935 0.0 36 2.2140156043819797E-4 0.0 0.0 8.827058813110515 0.0 37 2.2140156043819797E-4 0.0 0.0 9.026394018025039 0.0 38 2.95202080584264E-4 0.0 0.0 9.22934544842672 0.0 39 2.95202080584264E-4 0.0 0.0 9.427868847619639 0.0 40 3.6900260073032996E-4 0.0 0.0 9.661004690761061 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGTA 35 1.01974365E-7 46.000004 10 TGTTGCG 75 0.0 46.000004 1 TTACGCG 35 1.01974365E-7 46.000004 1 ATACGCG 75 0.0 46.000004 33 AATCGGA 35 1.01974365E-7 46.000004 44 TATCCGT 35 1.01974365E-7 46.000004 13 CGGACCC 35 1.01974365E-7 46.000004 33 GTACCTA 25 3.4174827E-5 46.0 10 TCGCACC 20 6.3118566E-4 46.0 44 ACGTTTA 25 3.4174827E-5 46.0 29 CGACGGT 95 0.0 46.0 28 CAACGAA 20 6.3118566E-4 46.0 45 ACGCATG 20 6.3118566E-4 46.0 1 TAGACGT 40 5.6134013E-9 46.0 35 GAGTCGA 20 6.3118566E-4 46.0 28 GCAACGC 25 3.4174827E-5 46.0 9 GACGTAG 50 1.6370905E-11 46.0 1 GAGTACG 45 3.110472E-10 46.0 1 ATAGCCG 25 3.4174827E-5 46.0 16 TATATCG 20 6.3118566E-4 46.0 29 >>END_MODULE