FastQCFastQC Report
Thu 26 May 2016
SRR1544702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544702_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1542794
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71500.46344489283728096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40410.2619273862874758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG650.046.01
TGTTTCG600.046.033
TCGTTAG405.6134013E-946.01
AGATCGT253.4177043E-546.038
GATCGTC253.4177043E-546.09
GTCGACA253.4177043E-546.020
CAACGTA301.8618211E-646.026
CAACGAC501.6370905E-1146.026
CGTGAGC405.6134013E-946.06
CGATTCG800.046.010
TAACGGA253.4177043E-546.022
GCGTTAT301.8618211E-646.040
CCGCTAG206.312131E-446.010
CGAGGTC301.8618211E-646.043
TCGTCAA301.8618211E-646.016
TGTTACG206.312131E-446.01
GAACGAC253.4177043E-546.011
CCGCGTA206.312131E-446.018
ACGTAAC206.312131E-446.035
TTATTCG501.6370905E-1146.018
Produced by FastQC (version 0.11.5)