FastQCFastQC Report
Thu 26 May 2016
SRR1544701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544701_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1277054
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69660.545474192947205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42540.33311042446129924No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT15230.11925885671240215Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14800.1158917320645799No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT14640.11463884847469252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACC351.0196891E-746.00000421
TACGTCC351.0196891E-746.00000423
TCACGGT301.8615665E-646.045
ACGCGAG650.046.01
GTCGAAA206.311719E-446.013
AGATACG301.8615665E-646.01
CAACGCG206.311719E-446.01
GCGAACA206.311719E-446.011
CGCATTA301.8615665E-646.029
CTATACG206.311719E-446.01
TAGCGCG551.8189894E-1246.01
ATCGTTC206.311719E-446.010
GCCGATT551.8189894E-1246.09
CGTAGTT301.8615665E-646.030
CGGATGT301.8615665E-646.016
GTATGCG650.046.01
AACGCCG206.311719E-446.013
AACGCAC206.311719E-446.027
GCGATAT850.046.09
GCGATAA405.6115823E-946.09
Produced by FastQC (version 0.11.5)